Surface display of OmpC of Salmonella serovar Pullorum on Bacillus subtilis spores

Abstract

Salmonellosis is a major public health problem throughout the world. Thus, there is a huge need for diversified control strategies for Salmonella infections. In this work, we have assessed the potential use of Bacillus subtilis (B. subtilis) spores for the expression of a major protective antigen of Salmonella serovar Pullorum, OmpC. The expression of OmpC on the surface of spores was determined by immunofluorescence microscopy. Mice immunized with recombinant spores expressing the OmpC antigen presented significant levels of OmpC-specific serum IgG and mucosal SIgA antibodies than in mice immunized with non-recombinant spores (p<0.01). In addition, oral immunization with recombinant spores was able to induce a significant level of protection in mice against lethal challenge with Salmonella serovar Typhimurium. These results suggest that B. subtilis spores have promising potential in the development of mucosal vaccines against Salmonella infections.

Progress in research and application development of surface display technology using Bacillus subtilis spores

“…Dai et al have assessed the potential use of B. subtilis spores for the expression of a major protective antigen of Salmonella serovar pullorum, OmpC. Mice immunized with recombinant spores expressing the OmpC antigen presented significant higher levels of OmpC-specific serum IgG and mucosal SIgA antibodies than mice immunized with nonrecombinant spores (p < 0.01) (Dai et al 2018). These results indicate that B. subtilis spores have broad applicability in vaccine development.…”

Surface display system for probiotics and its application in aquaculture

“…Several kinds of protein such as OmpC of Salmonella (Dai et al . 2018) and paramyosin from Clonorchis sinensis (Sun et al . 2018) were fused with cotC.…”

Avaliação Da Adequação Das Boas Práticas De Fabricação E Qualidade De Carne Bovina Em Açougues

“…Os resultados apresentados consideraram a totalidade de estabelecimentos (n=40), ou seja, 520 itens avaliados (13 itens em cada estabelecimento e 40 estabelecimentos). Para a determinação do pH, utilizou-se 50 g de cada amostra homogeneizada com 10 mL de água destilada, sendo a leitura realizada através do potenciômetro de um pHmetro de bancada previamente calibrado (Brasil, 1981 Os isolados suspeitos foram confirmados pela técnica de PCR, tendo como alvo o gene ompC (Dai et al, 2018). Através do método de fervura (Zanetti et al, 2019) extraiuse o DNA, que foi utilizado em reação posterior composta por GoTaq Green Master Mix, água estéril livre de nuclease e primer(i) (ompC-F 5"-ATCGCTGACTTATGCA ATCG-3", ompC-R 5"-CGGGTTGCGTTATAGGTCTG-3").…”