Surface Coassembly of Polymer Brushes and Polymer–Protein Bioconjugates: An Efficient Approach to the Purification of Bioconjugates under Mild Conditions

Hou, Wangmeng; Lin, Wei; Liu, Li; Zhao, Hanying

doi:10.1021/acs.biomac.8b01355

Cited by 23 publications

(39 citation statements)

References 43 publications

(57 reference statements)

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“…We ando ther researchers have demonstrated that the conjugation of proteins to thermally responsivep olymers could change the phase-transition temperatures of the polymers significantly. [21][22][23][24] The shift in the phasetransition temperature of PNIPAM in PPL nanogelsi sa ttributed to the incorporation of PPL molecules to the network structures and an increase in the hydrophilicity of the polymer. After coating with BSA, the endotherm of PNIPAM shifts to the high-temperature regime.…”

Section: Resultsmentioning

confidence: 99%

Biomimetic Mineralization of Protein Nanogels for Enzyme Protection

Liu

et al. 2019

Chemistry A European J

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Protein nanogelsh ave found aw idev ariety of applications, ranging from biocatalysis to drug/protein delivery. However,i np ractical applications, proteinsi nn anogelsm ay suffer from enzymic hydrolysis and denaturation. Inspired by the structure and functionalities of the fowl eggshells, biomimetic mineralization of protein nanogels was studied in this research. Protein nanogelsw ith embedded porcine pancreas lipase (PPL) in the cross-linked nanostructures were synthesized throught he thiol-disulfide reactionb etween thiol-functionalized PPL and poly(N-isopropylacrylamide) with pendant pyridyl disulfide groups.T he nanogels were further reacted with reducedb ovine serum albumin( BSA) and BSAmolecules were coated on the nanogels. Mineraliza-tion of BSA leads to the synthesis of biomineralized shells on the nanogels. With the growth of CaCO 3 on the shells, the nanogels aggregate into suprastructures. Thermogravimetric analysis, XRD, dynamic light scattering, and TEM were employed to study the mechanismo ft he biomineralization process and analyze the structureso ft he mineralized nanogels. The biomineralized shells can effectively protect the PPL molecules from hydrolysis by trypsin;m eanwhile, the nanosized channels on the mineralized shells allow the transport of small-molecule substrates across the shells.B ioactivity measurements indicatet hat PPL in the nanogels maintains more than 80 %b ioactivity after biomineralization.[a] Q.

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Section: Resultsmentioning

confidence: 99%

Biomimetic Mineralization of Protein Nanogels for Enzyme Protection

Liu

et al. 2019

Chemistry A European J

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“…Surface co‐assembly method can be applied in the fabrication of biosurface. In a recent publication, Hou and coworkers reported the synthesis of biosurface and protein purification by co‐assembly of polymer‐protein bioconjugates and polymer brushes on silica particles . Bovine serum albumin‐poly(N‐isopropylacrylamide) (BSA‐PNIPAM) bioconjugate was used as a model bioconjugate.…”

Section: Surface Nanostructures Fabricated By Surface Co‐assembliesmentioning

confidence: 99%

Surface Nanostructures Based on Assemblies of Polymer Brushes

2020

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Studies on the fabrication of hierarchical surface nanostructures have significantly promoted the development of materials with new surface properties and functionalities. In this Minireview, progress on the fabrication of surface nanostructures based on surface organization of polymer brushes are outlined. In the past decades, self-assemblies of polymer brushes, including homopolymer, block-copolymer and mixed-polymer brushes, have aroused great interest in the fields of chemistry and materials science. Recent studies demonstrate that surface co-assemblies of polymer brushes and free polymer chains on solid surfaces are considered as an efficient approach to the preparation of hierarchical nanostructures. Some typical surface nanostructures and properties achieved through surface coassembly approach are reviewed in this article. Meanwhile, the fundamental problems in the co-assembly approach are discussed and the potential applications of the hierarchical surface nanostructures are presented.

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“…[10] Herein, we demonstrate that coassembly of homopolymer brushes and block copolymer chains can be used to control the surface composition and wetting behavior of the solid surfaces.S urface micelles on silica particles are prepared by the coassembly approach, and the cores are crosslinked by the photodimerization of anthracene (Scheme 1). [10] Herein, we demonstrate that coassembly of homopolymer brushes and block copolymer chains can be used to control the surface composition and wetting behavior of the solid surfaces.S urface micelles on silica particles are prepared by the coassembly approach, and the cores are crosslinked by the photodimerization of anthracene (Scheme 1).…”

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“…As determined by size exclusion chromatography (SEC), the apparent molecular weights (Mns) and molecular weight dispersities (PDIs) of the two polymers are 13.2 K, 1.24 and 24.5 K, 1.18 ( Figure S2). [10,11] Silica particles with an average size of 180 nm were synthesized by the Stçber method ( Figure S3), [12] and the particles were decorated with pyridyl disulfide groups (SiO 2ss-Py) by two-step surface reactions. Based on 1 HNMR analysis ( Figure S1b), the repeating unit number of DMAEMA in the block copolymer was determined to be 60.…”

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Surface Reconstruction by a Coassembly Approach

2019

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Materials with switchable surfaces,c apable of changing surface properties under external stimuli, are playing apivotal role in many applications,such as tissue engineering, biosensors,a nd drug/protein delivery.I nt his researchs ilica particles with patterned and switchablesurfaces are fabricated. Surface micelles on silica particles are formed by coassembly of polymer brushes and "free" blockc opolymer chains in as elective solvent. The cores of the surface micelles are crosslinked by anthracene photodimerization. After quaternization of the coronae,a mphiphilic surface micelles are prepared. The surface micelles are able to rearrange in different media. After treatment with an organic solvent, the surfaces of silica particles are occupied by hydrophobic polymer components;i na queous solution, the positively charged polymer chains are on the surfaces.T he switching of the surface micelles results in changes in surface composition and wetting behaviors.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.

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Surface Coassembly of Polymer Brushes and Polymer–Protein Bioconjugates: An Efficient Approach to the Purification of Bioconjugates under Mild Conditions

Cited by 23 publications

References 43 publications

Biomimetic Mineralization of Protein Nanogels for Enzyme Protection

Biomimetic Mineralization of Protein Nanogels for Enzyme Protection

Surface Nanostructures Based on Assemblies of Polymer Brushes

Surface Reconstruction by a Coassembly Approach

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