Surface chemical modification of poly(phthalazinone ether nitrile ketone) through rhBMP-2 and antimicrobial peptide conjugation for enhanced osteogenic and antibacterial activities in vitro and in vivo

Li, Wentao; Liu, Chengde; Liu, Cheng; Li, Yizheng; Pan, Liang; Wang, Jinyan; Jian, Xigao

doi:10.1016/j.cej.2021.130321

Cited by 17 publications

(11 citation statements)

References 50 publications

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“…The spin-coated samples were then dried and immersed in 1.5× SBF at 37 °C for 1, 3, and 7 days. 45 Characterization of the Synthetic Polymer and Surface-Modified Substrate. Structural Characterization and Chemical Analysis of Synthetic Polymers.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Phosphonate-Functionalized Poly(phthalazinone ether ketones) Induce the Formation of Apatite Coatings for Enhanced Biocompatibility and Osteogenic Activity

Cheng

et al. 2022

ACS Appl. Polym. Mater.

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Poly(phthalazinone ether ketone) (PPEK), a poly(aryl ether ketone) (PAEK), has good thermal stability, nitrogen-containing functional groups, and bone-like mechanical properties. However, its surface biological inertness limits its application in biomedical applications. This paper presents the synthesis of PPEK modified with quantitative introduction of phosphonate groups (PPPEK) by a simple wet chemical modification method. The biomineralization process is regulated by the number of phosphonate groups, and a coating similar to bone apatite is deposited on the surface of PPEK substrates, thus greatly improving the biocompatibility of the material surface. The chemical structures of PPPEK are confirmed by 1 H NMR and FTIR. Microstructural and compositional studies performed by SEM, TEM, XPS, and XRD reveal that apatite coating is significantly enhanced with the increase in the phosphonate groups on the PPEK substrate. In addition, cell culture studies show that apatite coating induced by PPPEK promoted cell viability, cell adhesion, and osteogenesis of MC3T3-E1 cells.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…They were called P50/PPEK and P100/PPEK, respectively. The spin-coated samples were then dried and immersed in 1.5× SBF at 37 °C for 1, 3, and 7 days …”

Section: Methodsmentioning

confidence: 99%

Phosphonate-Functionalized Poly(phthalazinone ether ketones) Induce the Formation of Apatite Coatings for Enhanced Biocompatibility and Osteogenic Activity

Cheng

et al. 2022

ACS Appl. Polym. Mater.

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“…The PPENK substrate was cut into small square pieces of 1 cm × 1 cm, which were sonicated in acetone, ethanol, and water for 10 min successively to remove surface grease and dust. 36 After PPENK squares were soaked in solvent for different time intervals (1, 3, 5, and 10 min), they were quickly removed and immersed in ice water for 30 min. Then, they were removed and rinsed with deionized water to obtain a PPENK substrate with 3D nanoporous structure (PPENK3).…”

Section: Methodsmentioning

confidence: 99%

“…PPENK substrate was obtained by a hot press molding method. The PPENK substrate was cut into small square pieces of 1 cm × 1 cm, which were sonicated in acetone, ethanol, and water for 10 min successively to remove surface grease and dust . After PPENK squares were soaked in solvent for different time intervals (1, 3, 5, and 10 min), they were quickly removed and immersed in ice water for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Stabilization of Apatite Coatings on PPENK Surfaces by Mechanical Interlocking to Promote Bioactivity and Osseointegration In Vivo

Cheng

Yang

Liu

et al. 2022

ACS Appl. Mater. Interfaces

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Apatite coatings with high stability can effectively improve the surface bioactivity and osteogenic activity of implant materials. In clinical practice, the ability of apatite coatings to bond with the substrate is critical to the effect of implants. Here, we propose a strategy to construct a three-dimensional (3D) nanoporous structure on the surface of a poly(phthalazinone ether nitrile ketone) (PPENK) substrate and introduce a polydopamine (PDA) coating with grafted phosphonate groups to enhance the overall deposition of a bone-like apatite coating in the 3D nanoporous structure during mineralization. This method leads to a mechanical interlocking between the apatite coating and the substrate, which increases the stability of the apatite coating. The apatite coating confers a better bioactive surface to PPENK and also promotes osteogenic differentiation and adhesion of MC3T3-E1 osteoblasts in vitro. The samples are then implanted into rat femurs to characterize in vivo osseointegration. Micro-CT data and histological staining of tissue sections reveal that PPENK with a stable apatite coating induces less fibrous capsule formation and no inflammatory response and promotes osteogenic differentiation and bone-bonding strength. This enhances the long-term use of PPENK implant materials and shows great potential for clinical application as orthopedic implants.

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“…Poly(phthalazinone ether nitrile ketone) (PPENK) bone implants with 3D porous structures were modified with recombinant human bone morphogenetic protein-2 (rhBMP-2) and newly synthesized cationic antimicrobial polypeptides (AMP-1, AMP-2) using click chemistry. 308 The modified implants prevent the adhesion of bacteria and promote bone regeneration due to the combined effect of rhBMP2 and AMP-2. The modified implants decreased the growth of fibrous tissue and facilitated the largest amount of high-density bone formation in a rabbit femur defect model post 12 days of implantation.…”

Section: Bone Regenerationmentioning

confidence: 99%

Antimicrobial peptide-based materials: opportunities and challenges

et al. 2022

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Surface chemical modification of poly(phthalazinone ether nitrile ketone) through rhBMP-2 and antimicrobial peptide conjugation for enhanced osteogenic and antibacterial activities in vitro and in vivo

Cited by 17 publications

References 50 publications

Phosphonate-Functionalized Poly(phthalazinone ether ketones) Induce the Formation of Apatite Coatings for Enhanced Biocompatibility and Osteogenic Activity

Phosphonate-Functionalized Poly(phthalazinone ether ketones) Induce the Formation of Apatite Coatings for Enhanced Biocompatibility and Osteogenic Activity

Stabilization of Apatite Coatings on PPENK Surfaces by Mechanical Interlocking to Promote Bioactivity and Osseointegration In Vivo

Antimicrobial peptide-based materials: opportunities and challenges

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