Surface chemical analysis and chromatographic characterization of polyethylenimine-coated hydroxyapatite with various amount of polyethylenimine

Murakami, Yūichiro; Sugo, Ken; Hirano, Minoru; Okuyama, Tsuneo

doi:10.1016/j.talanta.2011.06.004

Cited by 20 publications

(17 citation statements)

References 15 publications

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“…PEI can establish a nondistorting and very strong ionic interaction with protein [11], and has been used to increase protein loading on the solid surface [12]. Techniques of electrostatic adsorption [13], spray drying [14], covalent graft [12] and layer-by-layer assembly [15] have been developed to introduce PEI on surface of inorganic or organic solid sphere. Spray drying often is used as an encapsulation technique by the food [16] and pharmaceutical industry [17].…”

Section: Introductionmentioning

confidence: 99%

“…Spray drying often is used as an encapsulation technique by the food [16] and pharmaceutical industry [17]. Murakami et al made use of the technique to produce PEI-coated hydroxyapatite with various fractions of PEI [14]. The correlation between coating fraction and the retention time of protein or nucleotide in the chromatographic column was revealed.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Polyamine Microsphere and Its Adsorption for Protein

Wang

Liu

Nie

et al. 2012

IJMS

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A novel polyamine microsphere, prepared from the water-in-oil emulsion of polyethylenimine, was characterized. The investigation of scanning electron microscopy showed that the polyamine microsphere is a regular ball with a smooth surface. The diameter distribution of the microsphere is 0.37–4.29 μm. The isoelectric point of the microsphere is 10.6. The microsphere can adsorb proteins through the co-effect of electrostatic and hydrophobic interactions. Among the proteins tested, the highest value of adsorption of microsphere, 127.8 mg·g−1 microsphere, was obtained with lipase. In comparison with other proteins, the hydrophobic force is more important in promoting the adsorption of lipase. The microsphere can preferentially adsorb lipase from an even mixture of proteins. The optimum temperature and pH for the selective adsorption of lipase by the microsphere was 35 °C and pH 7.0.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of a Polyamine Microsphere and Its Adsorption for Protein

Wang

Liu

Nie

et al. 2012

IJMS

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“…2(a), the weight-loss stage at ~70 C would be attributed to the desorption of adsorbed water. 32 The two weak weight-loss peaks at 237 and 338 C might be attributed to the desorption of amine (-NH2) and imine (-NH-) groups on TEPA-NCMs, respectively. The weight-loss at 432 C could be attributed to the decomposition of polymer.…”

Section: Ir Spectra and Tg/dtg Studiesmentioning

confidence: 99%

Amino-functionalized Nano-size Composite Materials for Dispersive Solid-Phase Extraction of Phosphate in Water Samples

et al. 2012

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An efficient analytical method for the preconcentration and determination of phosphate in water samples at trace levels was proposed. The method was based on sample enrichment using dispersive solid-phase extraction (dSPE) with tetraethylenepentamine (TEPA)-functionalized nano-size composite materials (TEPA-NCMs) as sorbents, which were fully characterized. Various parameters affecting the extraction efficiency were systematically investigated. After extraction, the post-adsorbed TEPA-NCMs were eluted by a NaOH solution for desorption of the phosphate. The resulting eluate containing phosphate was determined by a spectrophotometric method. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.29 and 0.96 μg L -1 , respectively. The relative standard deviations (RSDs) were lower than 8.0% with average recoveries ranging from 91 to 118%. The present method was successfully applied to the determination of phosphate at trace levels in real water samples, and it was confirmed that the TEPA-NCMs are highly effective dSPE materials.

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“…8 are very interesting from the perspective of previous mixed-mode separations [24] 2.8-11.7 6 1.86-2.88 × 10 −2 NP Resin particle fabricated and PEI modified cryogel monoliths [25] 2.4-11.2 8 0.75-1.34 × 10 −2 NP PEI modified PMA-EDMA monoliths [22] 14.3 6 NP 800 PGMA-DVB-PEI600 Resin [28] 11-26 6 NP 400-800 NP: not provided. on native nylon 6 C-CP fibers [12,13,50].…”

Section: Rapid Separation Of Proteins As a Function Of Linear Velocitymentioning

confidence: 99%

“…An amine-rich polymer, polyethylenimine (PEI), has been widely used in the chemical modifications of chromatography stationary phases for anion exchange (AEX) separation due to its low-cost and easy availability. PEI has been adsorbed on inorganic supports, including silicas [20,21], zirconia [22,23], graphite [24], and hydroxyapatite [25], followed by cross-linking for ionexchange chromatography. On organic supports, PEI is usually reacted with surface anchor groups and covalently bound onto the surface.…”

Section: Introductionmentioning

confidence: 99%