Surface Antigens on Eosinophils in Bronchial Asthma and Eosinophilic Pneumonia

Sakamoto, Shoichi; Oki, Kazuhiko; Takahashi, Hisaho; Arakawa, Yukako; Sugita, Hironobu; Kawabata, Y; Takeuchi, Kazuhiko; Tomichi, Nobukazu

doi:10.1159/000237189

Cited by 7 publications

(10 citation statements)

References 4 publications

(4 reference statements)

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“…Eosinophils in the sputum of asthmatics have been shown to express HLA-DR (Hansel et al 1991). Airway, but not blood, eosinophils in chronic eosinophilic pneumonia also have been demonstrated to express HLA-DR (Beninati et al 1993, Okubo et al 1995, Sakamoto et al 1995b). In patients with asthma, even blood eosinophils have been found to express greater HLA-DR than eosinophils from normals (Sakamoto et al 1995a).…”

Section: Eosinophils As Potential Antigen-present-ing Cellsmentioning

confidence: 99%

“…Airway, but not blood, eosinophils in chronic eosinophilic pneumonia also have been demonstrated to express HLA-DR (Beninati et al 1993, Okubo et al 1995, Sakamoto et al 1995b). In patients with asthma, even blood eosinophils have been found to express greater HLA-DR than eosinophils from normals (Sakamoto et al 1995a). Moreover, comparisons of blood and bronchoalveolar lavage eosinophils obtained 48 hr after segmental antigen (Sedgwick et al 1992) or 4-6 hr after inhalational (Mengelers et al 1994) challenges in allergic subjects have demonstrated that HLA-DR was expressed on airway eosinophils.…”

Section: Eosinophils As Potential Antigen-present-ing Cellsmentioning

confidence: 99%

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Human eosinophil-lymphocyte interactions

Weller

Lim

1997

Mem. Inst. Oswaldo Cruz

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Section: Eosinophils As Potential Antigen-present-ing Cellsmentioning

confidence: 99%

Section: Eosinophils As Potential Antigen-present-ing Cellsmentioning

confidence: 99%

Human eosinophil-lymphocyte interactions

Weller

Lim

1997

Mem. Inst. Oswaldo Cruz

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“…Based on the pattern of forward and side scatter, a granulocyte gate was set. Neutrophils were identified as CD9-negative granulocytes (Saito et al, 1986;Sakamoto et al, 1995). Data on CD9-positive eosinophils are not presented due to the low number of cells.…”

Section: Methodsmentioning

confidence: 99%

Effects of in vivo administration of G‐CSF on neutrophil and eosinophil adhesion

et al. 1997

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The effect in vivo of G‐CSF on neutrophil and eosinophil adhesion was studied after subcutaneous administration to six healthy individuals of human recombinant glycosylated G‐CSF (lenograstim) (3 μg/kg) for 6 consecutive days. Basal adhesion and adhesion to E‐selectin, VCAM‐1 and ICAM‐1 of neutrophil and eosinophil granulocytes were measured selectively. During G‐CSF administration neutrophil basal adhesion increased from 7.4 ± 3.9% (mean ± SD) to 55.8 ± 12.9% and 23.2 ± 4.4%, 4 and 7 d, respectively, after start of the administration. At the same time points eosinophil basal adhesion increased from 7.1 ± 2.4% to 37.7 ± 6.1% and 13.1 ± 5.3%, respectively. When adhesion was measured in the presence of Mn2+, which increases the functional activity of integrins, an even higher increase of neutrophil and eosinophil basal adhesion was noted 4 and 7 d, respectively, after start of G‐CSF administration. In parallel with the enhanced basal adhesion neutrophil adhesion to E‐selectin and ICAM‐1 and eosinophil adhesion to E‐selectin, VCAM‐1 and ICAM‐1 were significantly (P < 0.05) increased after 4 d of G‐CSF administration as was neutrophil cell surface expression of CD11b and CD18. In vitro G‐CSF induced minimal changes of granulocyte basal adhesion and inhibition of the adhesion to E‐selectin. 10 ng/ml TNFα significantly increased neutrophil and eosinophil basal adhesion and adhesion to VCAM‐1 and ICAM‐1. In summary, administration of G‐CSF to healthy subjects induced enhanced adhesion of neutrophil and eosinophil granulocytes, probably mediated by an increase of the functional capacity of β1‐ and β2‐integrins. The induction of increased levels of TNFα might be one mechanism behind the in vivo effect of G‐CSF administration.

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“…After lysis o f red cells, peripheral blood leukocytes were washed thoroughly with phosphatebuffered saline and analyzed by flow cytometry' with FACScan (Bccton Dickinson) according to a new method described previously [3]. Briefly, eosinophils were identified by positivity for CD9 (Nichirei, Tokyo) in the granulocyte fraction gated by autofluorescence (eosino phils were more than 99% in purity), and were analyzed for expres sion of HLA-DR, CD4, C D llb , CD44 and CD45RO (Nichirei, Tokyo).…”

Section: Aterials and M Ethodsmentioning

confidence: 99%

“…Recent studies have suggested that blood eosinophils from normal control subjects and from patients with eosinophilia vary with respect to their chemotactic re sponses [1], cytotoxic ability [2] and surface antigens [3]. This heterogeneity of eosinophils may be associated with differences in inflammatory environment or in cytokine expression patterns.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Surface Antigens on Eosinophils from Patients with Eosinophilia

Sakamoto¹,

Oki

Takahashi

et al. 1996

Int Arch Allergy Immunol

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Recent studies have suggested that there may be heterogeneity among human eosinophils. To study this further, surface antigens on blood eosinophils from patients with eosinophilia (23 bronchial asthma, 6 eosinophilic pneumonia, 1 Kimura’s disease and 1 adult T-cell leukemia) and from 8 control subjects were examined using a new direct method for fluorescence detection of eosinophils. HLA-DR+ and CD4+ eosinophil counts were higher in patients with bronchial asthma and adult T-cell leukemia (ATL) than in patients from other groups and in control subjects. CD1 lb+ eosinophil counts in Kimura’s disease and ATL were smaller than those in the other groups. CD45RO+ eosinophil counts in bronchial asthma and eosinophilic pneumonia were significantly higher (p < 0.05) compared with Kimura’s disease, ATL and control subjects. CD44+ eosinophil counts in eosinophilic pneumonia were significantly higher (p < 0.05) compared with the other groups and control subjects. These results suggest the existence of functional heterogeneity in the different eosinophilic diseases, with eosinophils in bronchial asthma and eosinophilic pneumonia being more highly activated in migration, activation and immunoregulation. On the other hand, eosinophils in Kimura’s disease and ATL might be functionally down-regulated. This heterogeneity of eosinophils may reflect differences in the pathogenesis of various eosinophilic diseases.

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Surface Antigens on Eosinophils in Bronchial Asthma and Eosinophilic Pneumonia

Cited by 7 publications

References 4 publications

Human eosinophil-lymphocyte interactions

Human eosinophil-lymphocyte interactions

Effects of in vivo administration of G‐CSF on neutrophil and eosinophil adhesion

Comparison of Surface Antigens on Eosinophils from Patients with Eosinophilia

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