Comparison of Surface Antigens on Eosinophils from Patients with Eosinophilia

Sakamoto, Shoichi; Oki, Kazuhiko; Takahashi, Hisaho; Arakawa, Yukako; Sugita, Hironobu; Hirano, Haruto; Takeuchi, Kazuhiko; Tomichi, Nobukazu; Sakuma, Tsutomu; Futai, Kazusige; Kawabata, Yoshinori

doi:10.1159/000237410

Cited by 6 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is up-regulated to a greater degree (1.5-1.8-fold by CF or about threefold by intensity) on BAL [63,100,117,118,164,171] and sputum (up to 18-fold) [119,120] eosinophils. However, blood eosinophil a M level is not significantly different among patients with mild and severe asthma, and normal subjects [77,112,119,125,161]. a X is increased (1.8-fold by intensity) on BAL [118] and (about threefold) on sputum [120] eosinophils but was found not to be different between subjects with asthma or airway allergy and normal subjects [77].…”

Section: Integrinsmentioning

confidence: 84%

“…These include CD9, whose expression on blood eosinophils from subjects with allergic rhinitis and occasional asthma was found to be higher during a high pollen load season (1.2-fold by fluorescence intensity) [170] but was in another study not different between subjects with asthma or airway allergy and normal subjects [77]. CD45RO expression on blood eosinophils is higher in patients with mild-moderate asthma than in normals (about 65% positive cells versus 5%) [125]. CD48 on blood eosinophils is up-regulated by IL-3 (about twofold by intensity), but not by IL-5 or GM-CSF, and is higher in patients with asthma (twofold) than in normal subjects [199].…”

Section: Othersmentioning

confidence: 93%

“…The eosinophil surface phenotype, consisting of numerous cell surface proteins, including adhesion molecules and cytokine, chemoattractant, complement, Fc, and innate immune receptors, has been reviewed extensively [5,12,13,[55][56][57]. Induction or up-regulation, in some cases, down-regulation, of a number of eosinophil surface proteins, for example CD69 and a M integrin (CD11b), as well as activated conformations of Fc receptors (FccRII = CD32) and integrins (b 1 and b 2 ) potentially report cell activation or have been proposed to be biomarkers in asthma (Table 1) Up-regulated by IL-5, after segmental antigen challenge, in BAL, or in sputum, higher in well-controlled than poorly controlled asthma [103,106,110,124] CD45RO Up-regulated in mild-moderate asthma [125] CD48…”

Section: General Remarksmentioning

confidence: 99%

“…Further, it is modestly up‐regulated on blood eosinophils after segmental lung antigen challenge (less than twofold by CF) and up‐regulated on BAL (5–6‐fold) and sputum (about 1.4‐fold by intensity) eosinophils. No differences have been found between CD44 expression on blood eosinophils in patients with asthma as a group and normal subjects . However, interestingly, Sano and colleagues found that the level of blood eosinophil CD44 expression was higher in patients with well‐controlled than poorly controlled asthma (about 1.7‐fold) and suggested that this implies that the transmigration of activated, CD44‐high eosinophils from the circulation is facilitated .…”

Section: Eosinophil Surface Proteins Proposed To Report Cell Activationmentioning

confidence: 99%

See 3 more Smart Citations

Activation states of blood eosinophils in asthma

Johansson

2014

Clin Experimental Allergy

134

147

View full text Add to dashboard Cite

Asthma is characterized by airway inflammation rich in eosinophils. Airway eosinophilia is associated with exacerbations and has been suggested to play a role in airway remodeling. Recruitment of eosinophils from the circulation requires that blood eosinophils become activated, leading to their arrest on the endothelium and extravasation. Circulating eosinophils can be envisioned as potentially being in different activation states, including non-activated, pre-activated or “primed”, or fully activated. In addition, the circulation can potentially be deficient of pre-activated or activated eosinophils, because such cells have marginated on activated endothelium or extravasated into the tissue. A number of eosinophil-surface proteins, including CD69, L-selectin, intercellular adhesion molecule-1 (ICAM-1, CD54), CD44, P-selectin glycoprotein ligand-1 (PSGL-1, CD162), cytokine receptors, Fc receptors, integrins including αM integrin (CD11b), and activated conformations of Fc receptors and integrins have been proposed to report cell activation. Variation in eosinophil activation states may be associated with asthma activity. Eosinophil-surface proteins proposed to be activation markers, with a particular focus on integrins, and evidence for associations between activation states of blood eosinophils and features of asthma are reviewed here. Partial activation of β1 and β2 integrins on blood eosinophils, reported by monoclonal antibodies (mAb) N29 and KIM-127, is associated with impaired pulmonary function and airway eosinophilia, respectively, in non-severe asthma. The association with lung function does not occur in severe asthma, presumably due to greater eosinophil extravasation, specifically of activated or pre-activated cells, in severe disease.

show abstract

Section: Integrinsmentioning

confidence: 84%

Section: Othersmentioning

confidence: 93%

Section: General Remarksmentioning

confidence: 99%

Section: Eosinophil Surface Proteins Proposed To Report Cell Activationmentioning

confidence: 99%

See 2 more Smart Citations

Activation states of blood eosinophils in asthma

Johansson

2014

Clin Experimental Allergy

134

147

View full text Add to dashboard Cite

show abstract

“…In the present study, marked expression of activation and adhesion molecules (CD45RO, CD18, CD11b) was detected on eosinophils in both patients, at diagnosis. Increased expression of CD45RO, CD11b has also been demonstrated to be suggestive of eosinophil activation in patients with asthma and allergic conditions (Thorne et al 1990;Yachie et al 1993;Sakamoto et al 1996). It has been shown that CD11b and CD18 play a role in migration of activated eosinophils to the site of allergic in¯ammation and in eosinophil adhesion to endothelial cells (Walsh 1997a;Yachie et al 1993 3 ).…”

Section: Discussionmentioning

confidence: 99%

The analysis of eosinophil and lymphocyte phenotype following single dose of high-dose methylprednisolone in two siblings with marked hypereosinophilia

Uçkan

Hiçsönmez

Tunç

et al. 2001

Clinical &Amp; Laboratory Haematology

View full text Add to dashboard Cite

The effect of single high-dose methylprednisolone (HDMP) on eosinophil and lymphocyte phenotype in two siblings with marked hypereosinophilia was investigated. Since conventional dose steroids failed to produce a haematological and clinical response in case one, both children were given HDMP (20 mg/kg/day). Eosinophils and lymphocytes showed an activated state before treatment, characterized by marked expression of CD11b, CD18, CD45RO on eosinophils and increased HLA-DR, CD95, CD18, CD38 on lymphocytes. Twenty-four hours after administration of HDMP a dramatic reduction in peripheral blood eosinophil count was observed in both patients associated with phenotypic changes characterized by decreased expression of CD11b, CD18, CD13 and increased CD95 expression in one. Furthermore, HDMP treatment induced a drop in the expression of CD95 and CD18 on lymphocytes. These changes may suggest a suppressive role for HDMP on eosinophil and lymphocyte activation which may have contributed to the haematological and clinical response.

show abstract