Eosinophil surface antigen expression and cytokine production vary in different ocular allergic diseases☆☆☆★★★

Hingorani, Melanie; Calder, Virginia L.; Jolly, Gilles; Buckley, Roger; Lightman, Susan

doi:10.1016/s0091-6749(98)70023-5

Cited by 83 publications

(61 citation statements)

References 60 publications

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“…Paraffin-embedded tissues were sectioned and processed for staining for galectin-9 using polyclonal antibody to galectin-9. An indirect streptavidin-biotin-alkaline phosphatase complex technique was used [16]. The sections were incubated for 60 min with 10% normal goat serum (Dako Ltd., High Wycombe, UK) to prevent nonspecific binding of antibody.…”

Section: Methodsmentioning

confidence: 99%

Association of Galectin-9 with Eosinophil Apoptosis

Saita

Goto

Yamamoto

et al. 2002

Int Arch Allergy Immunol

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Background: There is no information whether galectin-9 (a novel eosinophil chemoattractant) was associated with pathogenesis of eosinophilic disorders. Methods: We assessed the expression of galectin-9 with imunostaining and in situ hybridization both in the lesion of angiolymphoid hyperplasia with eosinophilia, and peripheral blood eosinophils of eosinophilic patients (E-Eos) in comparison with those of normal volunteers (N-Eos). Regulation of expression of galectin-9 on eosinophils and the effect of galectin-9 on apoptosis of eosinophil were also evaluated. Results: Many eosinophils infiltrating the site were positive for galectin-9. Surface and intracellular immunoreactive galectin-9 was more evident in E-Eos than N-Eos. When eosinophils were cultured with IL-5 in vitro, the surface galectin-9 expression of E-Eos was significantly downregulated, although that of N-Eos was not affected. Treatment of eosinophils with dexamethasone or anti-Fas antibody significantly upregulated the surface galectin-9 expression of E-Eos. In contrast, dexamethasone partially downregulated the surface galectin-9 of N-Eos, although anti-Fas antibody failed to affect on the surface galectin-9 expression. We also found that recombinant galectin-9 significantly suppressed apoptosis of E-Eos (p = 0.0431), whereas it apparently enhanced apoptosis of N-Eos (p = 0.0173). Furthermore, dexamethasone-induced apoptosis of N-Eos was significantly suppressed by galectin-9 (p = 0.0431), whereas galectin-9 failed to induce significant change in dexamethasone-induced apoptosis of E-Eos. In contrast, apoptosis induced by anti-Fas antibody in both N-Eos (p = 0.0431) and E-Eos (p = 0.0431) was enhanced by galectin-9. Conclusions: These findings suggested that galectin-9 was produced by eosinophils, and galectin-9 showed heterogeneous effects and kinetics to eosinophils, and this factor might be one of crucial factors in eosinophilic inflammation.

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Section: Methodsmentioning

confidence: 99%

Association of Galectin-9 with Eosinophil Apoptosis

Saita

Goto

Yamamoto

et al. 2002

Int Arch Allergy Immunol

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“…Recent studies have shown a prevalent local Th2 response in VKC, 5,6 with increased numbers of mast cells and eosinophils in the conjunctiva. 4,7 Eotaxin-1 and eotaxin-2, two eosinophil chemotactic and activating chemokines, seem to be implicated in the pathogenesis. 8 Increased levels of activated blood eosinophils and serum eosinophil cationic protein have been detected, indicating a systemic involvement.…”

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confidence: 99%

Long Eyelashes in a Case Series of 93 Children With Vernal Keratoconjunctivitis

et al. 2005

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ABSTRACT. Objective. Vernal keratoconjunctivitis (VKC) is a chronic conjunctivitis that affects children mainly in temperate areas, with exacerbations in spring and summer. Eyelashes provide natural protection for the eyes from sunshine, wind, and foreign bodies. These factors induce a worsening of signs and symptoms in VKC, whereas mechanical protection of the eyes produces relief. The aim of this study was to evaluate eyelash length in a large series of VKC patients and in ageand gender-matched healthy subjects.Methods. Upper eyelash length was measured in 93 VKC patients (mean age: 8.7 years; range: 4 -18 years) with a digital caliper. History, clinical form, type of presentation, degree of severity, and drug therapy were evaluated. Skin tests and serum-specific immunoglobulin E for common allergens, serum-total immunoglobulin E, peripheral blood eosinophil counts, and serum eosinophil cationic protein were determined. Two age-and gender-matched control subjects for each VKC patient underwent the same eyelash measurement.Results. The upper eyelashes were significantly longer in VKC patients than in control subjects (mean ؎ SD: 8.9 ؎ 1.17 mm vs 7.9 ؎ 1.07 mm). In healthy subjects, a negative correlation was found between eyelash length and age. With multivariate analysis, unlike control subjects, the eyelash length in VKC patients did not correlate with age, was positively correlated with the degree of severity of VKC, and was negatively correlated with the use of topical cyclosporine.Conclusions. Patients with VKC had longer eyelashes than healthy matched subjects. ABBREVIATIONS. VKC, vernal keratoconjunctivitis; bFGF, basic fibroblast growth factor; s-P, substance P; IgE, immunoglobulin E; s-ECP, serum eosinophil cationic protein; PBEC, peripheral blood eosinophil count.

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“…Th2 predominance is a widely accepted fact in the development of allergic diseases [1,2,[14][15][16] . The paradigm of Th1-Th2 is not characterized by separated pathways but by complex overlapping interractions between them [14] .…”

Section: Discussionmentioning

confidence: 99%

Inducing Ocular Allergy with β-Lactoglobulin: A New Experimental Model

Sari

Dinç

Yılmaz³

et al. 2010

Ophthalmic Res

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Purpose: To develop a new experimental ocular allergy animal model induced by β-lactoglobulin (BLG), a major cow’s milk allergen, and to discuss the clinical, histopathological and immunohistochemical findings. Methods: Forty Balb/c mice were randomized and separated into groups of 10. Groups were determined according to the different concentrations of BLG drops used. Study groups were immunized with 2.5, 5 or 10 mg/ml topical BLG (groups 2, 3 and 4, respectively) following intraperitoneal injection for the systemic immunization. The control group (group 1) was immunized with aluminium hydroxide (alum) alone within the same intervals. After ocular challenge, all the animals were evaluated clinically, histopathologically (mast cell and eosinophil infiltration) and immunhistochemically in terms of both T helper type 1- (IFNγ, TNFα) and T helper type 2- (IL-3, IL-4, IL-5, IL-10) specific cytokines. Results: Both clinical and immunohistochemical findings showed that an allergic conjunctivitis was induced in all study groups, with an optimized dosage of topical 5 mg/ml BLG. The conjunctivitis was associated with both Th1 and Th2 response, with a slight predominance of Th1 reaction. Conclusion: We describe a new murine model of acute allergic conjunctivitis induced by BLG. We believe that this new preliminary model has the immune parameters of the late phase of acute allergic conjunctivitis and it provides an alternative means for studying the pathogenesis and future treatments of ocular allergy. Our results should be enhanced with more detailed cellular and humoral parameters.

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Eosinophil surface antigen expression and cytokine production vary in different ocular allergic diseases☆☆☆★★★

Cited by 83 publications

References 60 publications

Association of Galectin-9 with Eosinophil Apoptosis

Association of Galectin-9 with Eosinophil Apoptosis

Long Eyelashes in a Case Series of 93 Children With Vernal Keratoconjunctivitis

Inducing Ocular Allergy with β-Lactoglobulin: A New Experimental Model

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