Supramolecular Fluorescent Probes for the Detection of Reactive Oxygen Species Discovered via High-Throughput Screening

Abstract

Supramolecular fluorescent probes for the detection of reactive oxygen species (ROSs) are designed based on a pro-guest strategy. Nine commercially available fluorescent dyes, six host molecules, and a pro-guest are used to rapidly generate a library of 54 potential supramolecular probes. These potential supramolecular probes are screened in a high-throughput fashion using a plate reader to discover seven “hits” or workable probes. The mechanism is confirmed to be ROS-induced conversion from a low-binding-affinity pro-guest to a high-binding-affinity guest and the competitive displacement of the encapsulated fluorescent dye. The response to H2O2 of four supramolecular probes is found to be concentration-dependent and may be used for quantitative analysis of H2O2. The supramolecular probe is selectively responsive toward other oxidative agents, such as NaClO and Na2SO3. The cell study shows that supramolecular probes are capable of detecting H2O2 in human cancer cells (MCF-7 or HeLa).

Oxidative Stress Parameters as Biomarkers of Cardiovascular Disease towards the Development and Progression

“…The detection of reactive oxygen species (ROS) has attracted interest in academic, medical, and industrial settings, particularly in elucidating its pathological mechanism and diagnosis [ 67 ]. To date, there are no universal methods for detecting ROS directly and consistently; the electron spin resonance (ESR) [ 68 , 69 ], mass spectrometry (MS) [ 70 ], spectrophotometry [ 71 , 72 ], high-performance liquid chromatography (HPLC) [ 73 ], fluorescence spectroscopy [ 74 ], and electrochemical techniques have been elucidated as an effective technique. Recent advancements in screening technologies enabled the development and enhancements of these technologies.…”

Recent Progress in Sensor Arrays: From Construction Principles of Sensing Elements to Applications

“…A novel electrochemical sensor array was constructed by modifying a 64-channel ITO electrode array with horseradish peroxidase and an electron transfer mediator, which has been applied to monitor the H 2 O 2 release from a rat hippocampal slice cultured at multiple positions . To achieve high-throughput screening of ROSs, a library of 54 potential supramolecular probes was fabricated using nine commercially available fluorescent dyes, six host molecules, and a pro-guest . ROSs induced the conversion from a low-binding affinity pro-guest to a high-binding-affinity guest and the competitive displacement of the encapsulated fluorescent dye.…”

“…134 To achieve highthroughput screening of ROSs, a library of 54 potential supramolecular probes was fabricated using nine commercially available fluorescent dyes, six host molecules, and a proguest. 135 ROSs induced the conversion from a low-binding affinity pro-guest to a high-binding-affinity guest and the competitive displacement of the encapsulated fluorescent dye. In addition, the supramolecular probes exhibited excellent selectivity to ROSs, and no response was observed for other common cations, anions, and biological molecules.…”

Acyclic cucurbit[n]uril-based nanosponges significantly enhance the photodynamic therapeutic efficacy of temoporfin in vitro and in vivo

“…Supramolecular interaction is a powerful tool to construct self-assembly systems. [30][31][32] Supramolecular nanoscale DDSs based on host-guest interaction are of great importance for the delivery of PDT PSs and other pharmaceuticals. [33][34][35][36] These supramolecular nanoscale DDSs have the advantage of facile preparation and simple loading due to the non-covalent interactions.…”