Suppressors of RNA silencing encoded by geminiviruses and associated DNA satellites

Rishishwar, Rashmi; Dasgupta, Indranil

doi:10.1007/s13337-018-0418-8

Cited by 14 publications

(9 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge, independent studies have identified that V2, C1, C2, C4/AC4 and βC1 encoded by different geminiviruses can suppress PTGS and/or TGS [ 31 , 32 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. Besides, previous reports have shown that V2 proteins encoded by begomoviruses with or without betasatellite have diverse functions for infection.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus

Jiang

et al. 2021

IJMS

View full text Add to dashboard Cite

V2 proteins encoded by some whitefly-transmitted geminiviruses were reported to be functionally important proteins. However, the functions of the V2 protein of tobacco curly shoot virus (TbCSV), a monopartite begomovirus that causes leaf curl disease on tomato and tobacco in China, remains to be characterized. In our report, an Agrobacterium infiltration-mediated transient expression assay indicated that TbCSV V2 can suppress local and systemic RNA silencing and the deletion analyses demonstrated that the amino acid region 1–92 of V2, including the five predicted α-helices, are required for local RNA silencing suppression. Site-directed substitutions showed that the conserved basic and ring-structured amino acids in TbCSV V2 are critical for its suppressor activity. Potato virus X-mediated heteroexpression of TbCSV V2 in Nicotiana benthamiana induced hypersensitive response-like (HR-like) cell death and systemic necrosis in a manner independent of V2′s suppressor activity. Furthermore, TbCSV infectious clone mutant with untranslated V2 protein (TbCSV∆V2) could not induce visual symptoms, and coinfection with betasatellite (TbCSB) could obviously elevate the viral accumulation and symptom development. Interestingly, symptom recovery occurred at 15 days postinoculation (dpi) and onward in TbCSV∆V2/TbCSB-inoculated plants. The presented work contributes to understanding the RNA silencing suppression activity of TbCSV V2 and extends our knowledge of the multifunctional role of begomovirus-encoded V2 proteins during viral infections.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus

Jiang

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The arms race between silencing and silencing suppression results in resistance or susceptibility to the pathogen. Geminiviruses encode several proteins namely AC2/C2, AC4/C4, AV2/V2, βC1, and Rep that suppress RNA silencing by targeting various components of the post-transcriptional gene silencing (PTGS) machinery, transcriptional gene silencing (TGS) machinery, and cellular regulatory genes (reviewed in Hanley-Bowdoin et al, 2013;Kumar, 2019;Rishishwar and Dasgupta, 2019;Yang et al, 2019;Guerrero et al, 2020). Of all the known geminiviral suppressor proteins, AC2 is the most well-studied and is known to target multiple plant genes and proteins.…”

Section: Plant Defense and Viral Counter-defense Mechanismsmentioning

confidence: 99%

Targets and Mechanisms of Geminivirus Silencing Suppressor Protein AC2

Veluthambi

Sunitha

2021

Front. Microbiol.

View full text Add to dashboard Cite

Geminiviruses are plant DNA viruses that infect a wide range of plant species and cause significant losses to economically important food and fiber crops. The single-stranded geminiviral genome encodes a small number of proteins which act in an orchestrated manner to infect the host. The fewer proteins encoded by the virus are multifunctional, a mechanism uniquely evolved by the viruses to balance the genome-constraint. The host-mediated resistance against incoming virus includes post-transcriptional gene silencing, transcriptional gene silencing, and expression of defense responsive genes and other cellular regulatory genes. The pathogenicity property of a geminiviral protein is linked to its ability to suppress the host-mediated defense mechanism. This review discusses what is currently known about the targets and mechanism of the viral suppressor AC2/AL2/transcriptional activator protein (TrAP) and explore the biotechnological applications of AC2.

show abstract

“…RNA silencing is considered as a major line of defense against pathogens such as viruses. Plants also have the capability to produce siRNA in response to dsRNA (Rishishwar and Dasgupta, 2019). However, most of the viruses encode proteins against this plant defense response.…”

Section: Relationship Of Clcuv With Whiteflymentioning

confidence: 99%

“…SiRNAs are formed when endonuclease dicer enzymes act on long dsRNA and cleave them which later bind with their complementary sequence for transcriptional or post transcriptional gene silencing. Contrarily, microRNAs are transcribed by their own genes or from fold back structures with double stranded regions from introns (Rishishwar and Dasgupta, 2019). In plants, these primary microRNAs are processed in the nucleus by the dicer enzymes into pre or precursor miRNA which later form duplexes before being exported to the RISCs (Sattar et al, 2013).…”

Section: Relationship Of Clcuv With Whiteflymentioning

confidence: 99%

Present status of cotton leaf curl virus disease (CLCUVD): a major threat to cotton production

Hasan

Rasul

Malik

et al. 2019

Int. J. Cotton Res. Technol.

View full text Add to dashboard Cite

Cotton is a major cash crop cultivated throughout the world for fiber, feed, fuel and food. Globally, it ranked 1st for fiber and 2nd among the oilseed crops. It is cultivated in around 60 countries of the world with an approximate production of 119.8 million bales annually. During its life cycle, cotton is exposed to about 75 destructive diseases from sowing till harvesting. These stresses are responsible for about 50% yield losses annually with a worth of $ 50 billion in the world. Among them, Cotton leaf curl virus disease (CLCuVD) is one of the significant factors responsible for yield reduction worldwide. This disease is caused by cotton leaf cur virus which is mainly acquired and transmitted by the vector Whitefly (Bemisia tabaci). This virus belongs to the family Geminiviridae and genus Begomovirus. Geminiviruses consist of small circular, single stranded DNA encapsidated in a twinned icosahedral pattern. These are either monopartite or bipartite. Bipartite begomoviruses are composed of circular single stranded DNA-A and DNA-B, both these components are essential for the infection in New World while some bipartite begomoviruses are also present in Old World, but differences are still present. Monopartite begomoviruses are composed of circular single stranded DNA-A and satellite molecules (DNA α-satellite and β-satellite), these are essential components for the severity of disease in the Old World. Efforts are underway to develop new strategies to protect the cotton plants against CLCuVD and its viral causal agents. This review helps the reader to better understand the biology of Cotton leaf curl virus (CLCuV), its behavior/interaction with the host Whitefly as well as recent developments being made for the control of cotton leaf curl disease.

show abstract

Suppressors of RNA silencing encoded by geminiviruses and associated DNA satellites

Cited by 14 publications

References 80 publications

Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus

Characterization of Pathogenicity-Associated V2 Protein of Tobacco Curly Shoot Virus

Targets and Mechanisms of Geminivirus Silencing Suppressor Protein AC2

Present status of cotton leaf curl virus disease (CLCUVD): a major threat to cotton production

Contact Info

Product

Resources

About