Suppressive effect of modified Simiaowan on experimental gouty arthritis: An in vivo and in vitro study

Shi, Le; Xu, Lina; Yang, Yanhua; Song, Hengwen; Pan, Hongying; Yin, Lian

doi:10.1016/j.jep.2013.10.023

Cited by 28 publications

(23 citation statements)

References 25 publications

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“…Besides, those genes such as IL-8, TNF-α, IL-1β, OAT1, GLUI9 (Rott and Agudelo, 2003;Lioté, 2003;Tausche et al, 2004), that may induce the occurrence of gout, could also be targeted by various ingredients in MSW. Experimental results show that MSW could play an important role in terms of anti-inflammation by inhibiting expression of ICAM-1, preventing neutrophil infiltration and apoptosis of HUVECs (Shi et al, 2013) and significantly reducing serum UA levels, together with decreasing liver XOD activity and IL-1β, IL-6 levels (Zeng et al, 2014). Thus, we consider that MSW may be effective in treating gouty diseases by cooperation of multiple targets and multiple pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Basic prescription (SM), which is described in a famous TCM monograph Danxi Xinfa (comprehensive medical book), has been used for hundreds of years in Chinese medicine for treating gouty arthritis with eliminating dampness, clearing heat and promoting blood circulation (Hua et al, 2012;Shi et al, 2013). Therefore, the network (Fig.…”

Section: Explore the Rationality Of Herb Combinations Of Mswmentioning

confidence: 99%

“…Recent researches have demonstrated that MSW could inhibit the occurrence of acute gout by suppressing the expression of intercellular cell adhesion molecule (ICAM-1), protecting HUVECs by reducing cell apoptosis, increasing cell viability in MSU-induced HUVECs, and weakening adhesion between neutrophil and endothelial cells (Shi et al, 2013). Moreover, MSW could significantly reduce UA levels in serum and urine in hyperuricemic model, together with decreasing liver XOD activity and IL-1β, IL-6 levels (Zeng et al, 2014) and has a better anti-gouty inflammation effect compared with indomethacin in the clinical study.…”

Section: Introductionmentioning

confidence: 99%

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A network pharmacology approach to determine active ingredients and rationality of herb combinations of Modified-Simiaowan for treatment of gout

Zhao

Yang³

et al. 2015

Journal of Ethnopharmacology

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Section: Discussionmentioning

confidence: 99%

Section: Explore the Rationality Of Herb Combinations Of Mswmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A network pharmacology approach to determine active ingredients and rationality of herb combinations of Modified-Simiaowan for treatment of gout

Zhao

Yang³

et al. 2015

Journal of Ethnopharmacology

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“…In traditional Chinese medicine, gout falls into the category of Bi syndrome caused by wind, cold, and dampness which was described in the Yellow Emperor's Classic of Internal Medicine. Previous evidence from clinical practice and experimental studies has confirmed that MSD has the potential to treat hyperuricemia and gouty arthritis [7, 8, 10, 29]. However, previous studies focused on urate lowing therapy.…”

Section: Discussionmentioning

confidence: 99%

Effects of Modified Simiao Decoction on IL‐1β and TNFα Secretion in Monocytic THP‐1 Cells with Monosodium Urate Crystals‐Induced Inflammation

Liu

Chen

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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Simiao pill, a Chinese herbal formula containing four herbs, has been used in the treatment of gouty arthritis for many years. The aim of this study was to explore the effects of modified Simiao decoction (MSD) on IL-1β and TNFα secretion in monocytic THP-1 cells with monosodium urate (MSU) crystals-induced inflammation. The MSU crystals-induced inflammation model in THP-1 cells was successfully established by the stimulation of phorbol 12-myristate 13-acetate (PMA) and MSU crystals. Then, the MSD-derived serum or control serum extracted from rat was administered to different treatment groups. The morphology of MSU crystals and THP-1 cells was observed. IL-1β and TNFα protein expression in supernatant of THP-1 cells were determined by ELISA. Our data demonstrated that MSU crystals induced time-dependent increase of IL-1β and TNFα. Moreover, MSD significantly decreased IL-1β release in THP-1 cells with MSU crystals-induced inflammation. These results suggest that MSD is promising in the treatment of MSU crystals-induced inflammation in THP-1 cells. MSD may act as an anti-IL-1 agent in treating gout. The underlying mechanism may be related to NALP3 inflammasome which needs to be validated in future studies.

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“…e original prescription will adjust some drugs [13] to achieve a better therapeutic effect. Due to Si Miao Wan not meeting actual clinical needs, many people have modified the Si Miao Wan based on the theory of TCM [14,15].…”

Section: Introductionmentioning

confidence: 99%

Effects and Mechanisms of Dendrobium officinalis Six Nostrum for Treatment of Hyperuricemia with Hyperlipidemia

Guo

Chen

Lei

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Objectives. Hyperuricemia (HUA) is a disease caused by increased production of uric acid (UA) or reduced excretion of UA in the body. Results of an epidemiological survey show that 60% of patients with HUA have hyperlipidemia (HPA). Dendrobium officinalis (DOF) six nostrum (DOS) is based on the theory of traditional Chinese medicine for the transformation of the traditional Chinese nostrum Si Miao Wan. In this article, we aim to discuss the efficacy and mechanism of DOS in reducing UA and regulating lipid metabolism. The rat model of HUA with HPA was induced by potassium oxonate (PO) combined with high-fat sorghum feed. We monitored the serum UA and blood lipids. Liver xanthine oxidase (XOD), adenosine deaminase (ADA), lipoprotein lipase (LPL), and fatty acid-binding protein (FABP1) activities were measured by enzyme-linked immunosorbent assay (ELISA) after the last administration of DOS. We performed a histopathological examination of rat kidney and intestine. Immunohistochemistry (IHC) was used to detect the expression of renal inflammatory proteins NLRP3 / Caspase-1 and intestinal inflammatory proteins TLR4 / NLRP3. We used western blot for measurement of liver hypoxanthine-guanine phosphoribosyl transferase (HPRT1) protein expression and renal PDZ domain protein kidney 1 (PDZK1) protein expression. DOS administration significantly reduced serum UA, total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-c) level, and improved liver steatosis in the model rat. At the same time, DOS treatment effectively inhibited liver XOD and ADA, increased the level of liver HPRT1, and reduced the production of UA. Additional studies had shown that DOS can restore normal UA excretion function in the intestine and kidney and regulated liver lipids metabolism. IHC and histopathological sections showed that DOS reduced the level of kidney, intestinal inflammatory body (NLRP3, Caspase-1, and TLR4), improved inflammation of the kidney and intestinal tract in rats. DOS is a promising drug that can effectively reduce serum UA and lipid level in the model rat. The mechanism of action may be related to inhibition of UA production, promotion of UA excretion, regulation of lipids metabolism, and anti-inflammatory response.

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Suppressive effect of modified Simiaowan on experimental gouty arthritis: An in vivo and in vitro study

Cited by 28 publications

References 25 publications

A network pharmacology approach to determine active ingredients and rationality of herb combinations of Modified-Simiaowan for treatment of gout

A network pharmacology approach to determine active ingredients and rationality of herb combinations of Modified-Simiaowan for treatment of gout

Effects of Modified Simiao Decoction on IL‐1β and TNFα Secretion in Monocytic THP‐1 Cells with Monosodium Urate Crystals‐Induced Inflammation

Effects and Mechanisms of Dendrobium officinalis Six Nostrum for Treatment of Hyperuricemia with Hyperlipidemia

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