Suppressive Effect of Modified Arabinoxylan from Rice Bran (MGN-3) onD-Galactosamine-Induced IL-18 Expression and Hepatitis in Rats

Abstract

We investigated in this study the effect of modified arabinoxylan from rice bran (MGN-3) and its fractions on D-galactosamine (D-GalN)-induced IL-18 expression and hepatitis in rats. Male Wistar rats were pretreated with MGN-3 or fractions of the MGN-3 hydrolysate, or with saline 1 h before administering D-GalN (400 mg/kg B.W.). The serum transaminase activities, IL-18 mRNA expression level in the liver and IL-18 concentration in the serum were determined 24 h after injecting D-GalN. Both the oral and intraperitoneal administration of MGN-3 (20 mg/kg B.W.) alleviated D-GalN-induced hepatic injury under these experimental conditions. The low-molecular-weight fraction (LMW) of MGN-3 showed the strongest protective effect on D-GalN-induced liver injury, its main sugar component being glucose. Moreover, the D-GalN-induced IL-18 expression was significantly reduced by treating with MGN-3 and LMW. The results suggest that MGN-3 and LMW could provide significant protection against D-GalN liver injury, and that IL-18 might be involved in their protective influence.

Protective effect of low molecular fraction of MGN-3, a modified arabinoxylan from rice bran, on acute liver injury by inhibition of NF-κB and JNK/MAPK expression

“…MGN-3 was hydrolyzed with HCl at 100°C, and then the hydrolysate treated with cation or anion exchange resin was fractionated by molecular weight (high molecular weight fraction (≥2,000,000 Da), medium molecular weight fraction (2,000,000-400 Da), and low molecular weight fraction (LMW; ≤400 Da)). We concluded that LMW has a stronger hepato-protective effect than MGN-3 [12]. The molecular weight of LMW was measured by ESIMS, and an intense peak at m/z 409 was observed [12].…”

“…We concluded that LMW has a stronger hepato-protective effect than MGN-3 [12]. The molecular weight of LMW was measured by ESIMS, and an intense peak at m/z 409 was observed [12]. LMW is a mixture of monosaccharide and oligosaccharides, constituted by glucose as the main component (glucose, 22.8%; mannose, 1.5%; galactose, 0.5%; arabinose, 0.3%; protein, 2.85%) [12].…”

“…LMW was prepared from MGN-3 as described previously in Zheng et al [12]. MGN-3 was hydrolyzed with 1 N HCl, at 100°C for 1 h, and then the hydrolysate treated with cation or anion exchange resin was fractioned in low molecular weight, riboflavin (M.W.…”

“…The molecular weight of LMW was measured by ESIMS, and an intense peak at m/z 409 was observed [12]. LMW is a mixture of monosaccharide and oligosaccharides, constituted by glucose as the main component (glucose, 22.8%; mannose, 1.5%; galactose, 0.5%; arabinose, 0.3%; protein, 2.85%) [12]. In our previous study, the results indicate that neutral oligosaccharides and monosaccharides in the LMW seem to be candidates for the effective ingredients for treating GalN-induced liver injury.…”

“…In mammals, 12 members of the TLR family have been identified [19]. TLR4 is a transmembrane protein, mainly existing in macrophages, which recognize LPS or LPS-CD14 complexes, and mediates macrophage activation and pro-inflammatory cytokine release [12,20]. CD14, a key gene of the innate immune system, functions as a receptor for LPS, a constitutive element of the bacterial cell wall.…”