Suppressive Effect of 4-Hydroxy-2-(4-Hydroxyphenethyl) Isoindoline-1,3-Dione on Ovalbumin-Induced Allergic Asthma

Abstract

4-Hydroxy-2-(4-hydroxyphenethyl)isoindoline-1,3-dione (PD1) is a synthetic phthalimide derivative of a marine compound. PD1 has peroxisome proliferator-activated receptor (PPAR) γ agonistic and anti-inflammatory effects. This study aimed to investigate the effect of PD1 on allergic asthma using rat basophilic leukemia (RBL)-2H3 mast cells and an ovalbumin (OVA)-induced asthma mouse model. In vitro, PD1 suppressed β-hexosaminidase activity in RBL-2H3 cells. In the OVA-induced allergic asthma mouse model, increased inflammatory cells and elevated Th2 and Th1 cytokine levels were observed in bronchoalveolar lavage fluid (BALF) and lung tissue. PD1 administration decreased the numbers of inflammatory cells, especially eosinophils, and reduced the mRNA and protein levels of the Th2 cytokines including interleukin (IL)-4 and IL-13, in BALF and lung tissue. The severity of inflammation and mucin secretion in the lungs of PD1-treated mice was also less. These findings indicate that PD1 could be a potential compound for anti-allergic therapy.

Peroxisome Proliferator-Activated Receptors as a Therapeutic Target in Asthma

“…Another PPAR agonist, namely, 4-hydroxy-12-(4-hydroxyphenethyl) isoindoline-1,3-dione, is a promising agent in asthma treatment. The experiment has revealed a decrease in the severity of inflammation and mucin secretion in the lungs of mice treated with this agonist [154].…”

Suppressive Effect of Carnosol on Ovalbumin-Induced Allergic Asthma

“…Rat RBL-2H3 cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured at 37°C in a 5% CO 2 -humidified incubator as described previously ( Huang et al ., 2018 ). Degranulation from RBL-2H3 cells was evaluated by measuring β-hexosaminidase release ( Lee et al ., 2018 ).…”

4-CMTB Ameliorates Ovalbumin-Induced Allergic Asthma through FFA2 Activation in Mice

“…Rat RBL-2H3 basophilic leukemia cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). RBL-2H3 cells were cultured at 37°C in a 5% CO2-humidified incubator, and maintained in 10% (v/v) heat-inactivated fetal bovine serum containing high-glucose Dulbecco's modified Eagle medium (DMEM) with 2 mM glutamine, 100 U/mL penicillin, 1 mM sodium pyruvate, and 50 µg/mL streptomycin (Huang et al, 2018).…”

“…Asthma is a chronic inflammatory airways disease, affecting several million people worldwide (Huang et al, 2018). Couphing, wheezing, chest tightness, variable airway obstruction, and airway hyper-responsiveness are main symptoms (Wardlaw et al, 2002).…”