Suppression subtractive hybridization PCR isolation of cDNAs from a Caribbean soft coral

Lopez, Jose V.; Ledger, A.; Santiago-Vázquez, Lory Z.; Pop, Mihai; Sommer, Dan; Ranzer, Llanie K.; Feldman, Robert A.; Kerr, Russell G.

doi:10.2225/vol14-issue1-fulltext-3

Cited by 1 publication

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Transcriptomic approaches have found that Acropora cervicornis affected by white band disease has higher expression of genes associated with immune function than healthy colonies (Libro et al 2013), and expression of the gene for an antimicrobial peptide was up-regulated on exposure to the coral pathogen Vibrio coralliilyticus in Pocillopora damicornis (Vidal Dupiol et al 2011). Transcriptomic studies of soft corals are rare (Lopez et al 2011, Woo et al 2012), but one study of G. ventalina identified differential expression of many genes in response to microbial pathogenesis (Burge et al 2013b). Likewise, many coral genes have demonstrated differential expression in response to exposure to lipo poly saccharide and other pathogen-associated molecular patterns, which are often used as proxies for pathogen challenge (Kvennefors et al 2010, Weiss et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of healthy and diseased hybrid soft corals Sinularia maxima × S. polydactyla

Gochfeld

Ankisetty²,

Slattery

2015

Dis. Aquat. Org.

View full text Add to dashboard Cite

Emerging diseases of marine invertebrates have been implicated as one of the major causes of the continuing decline in coral reefs worldwide. To date, most of the focus on marine diseases has been aimed at hard (scleractinian) corals, which are the main reef builders worldwide. However, soft (alcyonacean) corals are also essential components of tropical reefs, representing food, habitat and the 'glue' that consolidates reefs, and they are subject to the same stressors as hard corals. Sinularia maxima and S. polydactyla are the dominant soft corals on the shallow reefs of Guam, where they hybridize. In addition to both parent species, the hybrid soft coral population in Guam is particularly affected by Sinularia tissue loss disease. Using label-free shotgun proteomics, we identified differences in protein expression between healthy and diseased colonies of the hybrid S. maxima × S. polydactyla. This study provided qualitative and quantitative data on specific proteins that were differentially expressed under the stress of disease. In particular, metabolic proteins were down-regulated, whereas proteins related to stress and to symbiont photosynthesis were up-regulated in the diseased soft corals. These results indicate that soft corals are responding to pathogenesis at the level of the proteome, and that this label-free approach can be used to identify and quantify protein biomarkers of sub-lethal stress in studies of marine disease.

show abstract