Suppression of v- src Transformation by the drs Gene

Abstract

Previously, we isolated a novel gene, drs, which was downregulated by retroviral oncogenes such as v-src and v-K-ras, from a cDNA library of primary rat embryo fibroblasts. Experiments using a temperature-sensitive mutant of the v-src gene indicated that downregulation of drsmRNA was dependent on functional expression of v-Src. In addition, expression of drs mRNA was also reduced by serum stimulation of G0-arrested normal rat fibroblast cells. To clarify the function of the drs gene in cell transformation and proliferation, we introduced drs linked to a potent promoter into a normal rat cell line, F2408, and examined the effect of ectopic expression of exogenous drs on the transformation by the v-src gene and growth properties. Cells expressing exogenous drs gene showed significantly decreased efficiency of transformation by v-src irrespective of functional expression of v-Src kinase, while the growth rate and G1/S progression of the cells were not suppressed by expression of exogenous drs gene, indicating thatdrs has the ability to suppress v-srctransformation without disturbing cell proliferation.

Down‐regulation of drs mRNA in Colorectal Neoplasms

“…28) The superficial-type colorectal tumors are etiologically distinct from ordinary colorectal polypoid tumors and therefore may follow an alternative colorectal tumorigenesis pathway. 29) Inoue et al have previously reported that drs has the ability to suppress v-src and v-K-ras transformation without disturbing cell proliferation in the rat cell line F2408, 5) and Yamashita et al have reported that induction of the drs gene in colon cancer cell lines causes suppression of anchorage-independent growth of these cells. 9) To elucidate the role of the drs gene in distinct colorectal carcinogenesis, we examined expression of drs mRNA in normal mucosa, various types of adenomas and morphologically distinct adenocarcinomas by in situ hybridization.…”

“…[1][2][3] The drs gene was isolated as a suppressor gene for v-src transformation from a cDNA library of primary rat embryo fibroblasts. 4,5) The drs gene is also down-regulated by other retroviral oncogenes, such as v-fps, v-ras, v-mos, v-sis, and v-abl, but not by large T antigen of simian virus 40 (SV40) or by the E6 and E7 genes of human papillomavirus. The drs gene has an open reading frame encoding 464 amino acid residues.…”

Insights from nanomedicine into chloroquine efficacy against COVID-19

“…For example, the SARS-CoV virus identified in 2003 and the human coronavirus NL63 (HCoV-NL63) identified in 2004 bind to the angiotensin-converting enzyme 2 (ACE2) receptor, triggering endocytosisdriven cell entry 14,15 . Both clathrin-mediated and clathrin/caveolae-independent endocytosis mechanisms have been described for SARS-CoV entry in human cells 16,17 . SARS-CoV-2 might use similar ACE2mediated mechanisms of cell entry 18 .…”

Isolation of a novel mouse gene, mSVS‐1/SUSD2, reversing tumorigenic phenotypes of cancer cells in vitro

“…4b). Suppression of colony formation by Drs gene, one of the known tumor suppressor genes, (15) was even more pronounced than Fsvs-1. These results indicate that ectopic expression of SVS-1 suppresses growth of Ki3T3 cells.…”