Suppression of Ultraviolet B‐mediated Matrix Metalloproteinase Generation by Sorbus commixta Twig Extract in Human Dermal Fibroblasts

Abstract

Sorbus commixta is a traditional oriental medicinal plant that grows in East Asian countries such as Korea, Japan and China. The twig of S. commixta has been considered valuable for centuries to treat diseases including asthma, cough and other bronchial disorders. However, the effect of S. commixta twig extract on human skin has not been investigated well. The present study aimed at assessing the antiphotoaging effect of S. commixta twig ethanol extract (STE) on ultraviolet B (UVB)-induced matrix metalloproteinase (MMP) levels and its underlying mechanism in human dermal fibroblasts. In this study, we found that STE (12.5-50 μg mL ) treatment significantly inhibited UVB-induced MMP-1, MMP-2 and MMP-3 expression, concomitant with a downregulation of intracellular ROS generation. These effects might be associated with a STE-induced inhibition of the mitogen-activated protein kinase (MAPK) pathway. Furthermore, STE also downregulated UVB-induced c-Fos expression in a concentration-dependent manner, but had no inhibitory effect on c-Jun phosphorylation. Taken together, these results indicate that STE may be an antiphotoaging agent and that its effect may occur via its inhibition of MMPs expression and MAPK pathway activation.

Inhibitory Effect of Lupeol on MMPs Expression using Aged Fibroblast through Repeated UVA Irradiation

“…We investigated whether the expression of MMPs is closely related to the expression of the MAPK pathway signaling in the UVA‐induced senescent model. Because of the expression of MAPKs was relatively rapid as previously described , we measured these proteins within 30 min. As a result, phosphorylation of JNK was not confirmed, but phosphorylation of p38 increased with time at 5, 15 and 30 min after the last UVA exposure.…”

“…Matrix metalloproteases (MMPs) expression, which accelerates skin aging due to external stress on the skin, is induced along the MAPKs pathway . Mitogen‐activated protein kinases (MAPKs) phosphorylate specific serine and threonine amino acids of target proteins to regulate gene expression or control various cellular activities such as cell growth, proliferation, differentiation and death . MAPKs have three subgroups: ERK which regulates cell division and growth; JNK which is a transcriptional regulator; and p38 which is activated by inflammatory cytokines and environmental stress is associated with immunity .…”

“…At 72 h, the culture medium was collected and the expression of MMPs was analyzed by Western blotting . In addition, NHDF‐neo cells extracts were prepared and Western blotting was performed as described previously .…”

Antimelanogenic and Antimigration Properties of the Ethyl Acetate Fraction of Calendula officinalis Flowers on Melanoma Cells

“…HPLC was performed as previously described . The working conditions were as follows: mobile phase: solvents A (2% acetic acid in H 2 O) and B (0.5% acetic acid in 50% acetonitrile aqueous solution), a gradient mode (0–10 min, 35–35% of B; 10–50 min, 35–80% of B; 50–60 min, 80–100% of B; 60–75 min, 100–100% of B); detection: 254–365 nm; and flow rate: 1.0 mL min −1 .…”

Protective effect of the Rubus chingii Hu. fruit extract on ultraviolet B-induced photoaging via suppression of mitogen-activated protein kinases (MAPKs) in vitro

“…The relative amounts of MAPKs in the cell were assessed by Western immunoblotting [20]. HFF-1 cells (5×10 5 cells/well) were seeded in 6-well plates and pretreated with different concentrations of E-EA for 24 h. The cells were exposed to 60 mJ/cm 2 UVB irradiation.…”