Suppression of the Allergic Contact Reaction in The Guinea Pig by Cyclosporin A

Abstract

In a study of the effects of cyclosporin A on the allergic contact reaction to oxazolone in the guinea pig, a pronounced ability to suppress redness and edema and the dermal inflammatory cell infiltrate was demonstrated. At the generally recommended (human) organ transplantation immunosuppression dosage (20 mg/kg), a single dose gave no certain effect, but when repeated daily for 3 days, some suppression of the reaction was seen. A single 80 mg/kg dose produced marked suppression of erythema and edema and all components of the dermal cellular infiltrate. This effect was still evident, though less marked, 3 days after administration. The 80 mg/kg dose had no effect on the toxic contact reaction to croton oil. Of the agents we have previously tested (cyclophosphamide, methotrexate and azathioprine) with this model, cyclosporin A has by far the most marked capacity to suppress the allergic (cell-mediated) contact reaction, via a mechanism which would appear to involve immunomodulation rather than mere nonspecific anti-inflammatory effects.

Evaluation of the skin sensitizing potential of biodegradable magnesium alloys

“…In the literature, histological criteria of qualitative and quantitative evaluation of the cell infiltrate15, 26, 27 as well as the number of basophile cells28 have been used to evaluate allergenic skin reactions. It is further reported that until 48 h after the beginning of the challenge, the number of macrophage and lymphocyte cells diminished, while the number of basophile cells increased 26, 28, 29. An elevated basophile cell count is considered a cutaneous basophile hypersensitivity and represents the weakest form of type IV allergies according to the classification of Coombs and Gell 30.…”

“…It is further reported that until 48 h after the beginning of the challenge, the number of macrophage and lymphocyte cells diminished, while the number of basophile cells increased. 26,28,29 An elevated basophile cell count is considered a cutaneous basophile hypersensitivity and represents the weakest form of type IV allergies according to the classification of Coombs and Gell. 30 In the positive control group of this study using the standard allergen HCA, we found a similar percentage of basophile cells in 400 leucocytes (13.2%) compared to results presented in another study reporting the cutaneous basophile hypersensitivity (12.5%).…”

Effects of Clonidine on the Dermal Inflammatory Cell Response of Experimental Toxic and Allergic Contact Reactions and Intradermal Hypersensitivity

“…This model, which has previ ously been used to assess immunomodulating and anti-inflammatory capabilities of therapeutic agents [5], was then adapted to the study of the intradermal 'wheal and flare' (reagin mediated, 'type I') hypersen sitivity reaction to ovalbumin [6]. A further aim was to correlate the observed features of the inflammatory and mast cell infiltrates with a hypothesis of the method of action of clonidine in hypersensitivity reactions.…”