Topical Application of Cyclosporine on Guinea Pig Allergic Contact Dermatitis

Abstract

Topical cyclosporine applied to the test site substantially inhibited the elicitation reaction of contact sensitivity in the guinea pigs previously sensitized with 2,4-dinitrochlorobenzene (DNCB). This suppressive effect of the drug was short lived and reversible. Cyclosporine was not effective when given six hours or later after antigenic challenge to the test site. Cyclosporine had no effect on the toxic contact reaction in normal animals either to croton oil or to DNCB in high concentration. Cyclosporine applied topically to the challenge site also resulted in a reduction of retest and flare-up reactions of contact sensitivity to DNCB, but did not affect the production of generalized rash in the same animals. These results indicate that in the future local topical application of cyclosporine may make treatment of human cutaneous immune-mediated disorders a possibility without serious side effects.

Anti-inflammatory effect of topical nanocrystalline silver cream on allergic contact dermatitis in a guinea pig model

“…The acute hypersensitivity reaction was associated with a pronounced erythema and accompanying oedema of all the test sites and the reaction was fully developed 24 h after elicitation. This animal model has been used as a suitable model for ACD and appears to be an appropriate model to evaluate topical anti‐inflammatory compounds 16–21 …”

“…Contact dermatitis was induced, as described by several investigators 16–21 . Guinea pigs were shaved and four 2‐cm diameter areas were tattooed on their backs using a marker, two left and two right of the spinal midline.…”

“…Contact dermatitis was induced, as described by several investigators. [16][17][18][19][20][21] Guinea pigs were shaved and four 2-cm diameter areas were tattooed on their backs using a marker, two left and two right of the spinal midline. Animals were sensitized by applying 100 lL of 5% chloro-2, 4 dinitrochlorobenzene (DNCB, Acros Chemicals, Somerville, NJ, USA), dissolved in acetone to designated areas on the back.…”

Production of acute and chronic itch with histamine and contact sensitizers in the mouse and guinea pig

“…All animals had the area behind both ears shaved, and 24 h later guinea pigs were sensitized with 5% DNCB solution (DNCB dissolved in olive oil: ethanol mix 1:4) behind one ear whereas mice were sensitized with 1% DNCB solution to an area behind one ear (17).…”

Protective and curative effects of topically applied CX-659S, a novel diaminouracil derivative, on chronic picryl chloride-induced contact hypersensitivity responses

“…The contact hypersensitivity response (CHR) elicited by a single epicutaneous application of a contact sensitizing agent in a presensitized animal has been widely used as a test system to evaluate the effects of topical and systemic anti‐inflammatory compounds including corticosteroids and immunosuppressants 1–3 . These acute CHR models are simple, rapid and reproducible, and are thus suitable for preliminary screening purposes and for elucidating the mechanism of action of anti‐inflammatory compounds.…”