Suppression of Rice Stripe Virus Replication in Laodelphax striatellus Using Vector Insect-Derived Double-Stranded RNAs

Abstract

RNA interference (RNAi) has attracted attention as a promising approach to control plant viruses in their insect vectors. In the present study, to suppress replication of the rice stripe virus (RSV) in its vector, Laodelphax striatellus, using RNAi, dsRNAs against L. striatellus genes that are strongly upregulated upon RSV infection were delivered through a rice leaf-mediated method. RNAi-based silencing of peroxiredoxin, cathepsin B, and cytochrome P450 resulted in significant down regulation of the NS3 gene of RSV, achieving a transcriptional reduction greater than 73.6% at a concentration of 100 ng/µl and, possibly compromising viral replication. L. striatellus genes might play crucial roles in the transmission of RSV; transcriptional silencing of these genes could suppress viral replication in L. striatellus. These results suggest effective RNAi-based approaches for controlling RSV and provide insight into RSV-L. striatellus interactions.

Insight into aphid mediated Potato Virus Y transmission: A molecular to bioinformatics prospective

“…RNAi is a molecular technique that targets gene expression in organisms by producing and delivering dsRNA ( Figure 5 ) (Aalto et al, 2007 ; Carthew and Sontheimer, 2009 ; Lam et al, 2015 ; Kanakala and Ghanim, 2016 ). The RNAi pathway is activated by the introduction of dsRNA (Fang et al, 2020 ). When dsRNA strands are recognized in a cell, they are cleaved into small interfering RNA (siRNA, 19–24 nucleotides) by the RNase III ribonuclease Dicer.…”

“…RNAi-based suppression of chaperone-usher pathway (Cup) genes has been used for pest management (Shang et al, 2020 ) as well as physiological research, such as metamorphosis (Jan et al, 2017 ). RNAi has also been used to limit plant viral transmission by suppressing virus replication in its vector (Fang et al, 2020 ). In RNAi research, dsRNA delivery techniques including microinjection, feeding, and soaking have all been utilized to assure dsRNA delivery to insects.…”

Interplay of Rice Stripe Virus and Rice Black Streaked Dwarf Virus during Their Acquisition and Accumulation in Insect Vector

“…Rice stripe virus (RSV, genus Tenuivirus, family Phenuiviridae) and rice black streaked dwarf virus (RBSDV, genus Fijivirus, family Reoviridae) cause serious yield losses of rice in East Asian countries [1][2][3][4][5][6][7]. RSV usually causes chlorotic or necrotic stripes on newly developed leaves, then premature wilting and stunting, sometimes resulting in total crop loss [8][9][10]. RBSDV causes stunted stems and dwarfism in seedlings [11,12], especially severe growth retardation and excessive tillering, and typical rice grains do not form [13].…”