Suppression of inflammatory and fibrotic responses in allergic inflammation by the amniotic membrane stromal matrix

Solomon, Abraham; Wajngarten, Maurı́cio; Alviano, Francesco; Anteby, Irene; Elchalal, Uriel; Pe’er, Jacob; Levi‐Schaffer, Francesca

doi:10.1111/j.1365-2222.2005.02285.x

Cited by 72 publications

(46 citation statements)

References 40 publications

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“…Interestingly, cells derived from either the epithelial or mesenchymal layers of human amniotic membrane-derived cells (HAMCs) impair lymphocyte activation, dendritic cell maturation, and inflammatory cytokine production in vitro (9)(10)(11)(12)(13). Moreover, treatment with total amniotic membrane, amniotic-derived cells, or their conditioned medium was found to be protective in different models of inflammatory fibrotic conditions (9,12,14,15). On these bases, the use of HAMCs in the treatment of autoimmune diseases becomes an attractive possibility still to be explored.…”

Section: Conclusion Hamcs Have Emerged As Attractive Candidates For mentioning

confidence: 99%

Therapeutic Effect of Human Amniotic Membrane–Derived Cells on Experimental Arthritis and Other Inflammatory Disorders

Parolini

Souza-Moreira

O’Valle

et al. 2014

Arthritis & Rheumatology

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Objective. Rheumatoid arthritis (RA) is an autoimmune disease caused by loss of immunologic self tolerance and characterized by chronic joint inflammation. Cells isolated from human amniotic membrane (HAMCs) were recently found to display immunosuppressive properties. The aim of this study was to characterize the effect of HAMCs on antigen-specific T cell responses in RA patients and to evaluate their therapeutic potential in a preclinical experimental model of RA.Methods. We investigated the effects of HAMCs on collagen-reactive T cell proliferation and cytokine production, on the production of mediators

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Section: Conclusion Hamcs Have Emerged As Attractive Candidates For mentioning

confidence: 99%

Therapeutic Effect of Human Amniotic Membrane–Derived Cells on Experimental Arthritis and Other Inflammatory Disorders

Parolini

Souza-Moreira

O’Valle

et al. 2014

Arthritis & Rheumatology

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“…In veterinary field, the amniotic membrane has been employed for ocular surface reconstruction in dog (Arcelli et al 2009) and horse (Ollivier et al 2006, Plummer et al 2009) due to its ability to promote re-epithelialization, to reduce fibrosis (Solomon et al 2005), and to modulate angiogenesis (Dua et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Corradetti¹,

Meucci²,

Bizzaro³

et al. 2013

Reproduction

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Amnion and amniotic fluid (AF) are noncontroversial and inexhaustible sources of mesenchymal stem cells (MSCs) that can be harvested noninvasively at low cost. As in humans, also in veterinary field, presumptive stem cells derived from these tissues reveal as promising candidates for disease treatment, specifically for their plasticity, their reduced immunogenicity, and high anti-inflammatory potential. The aim of this work is to obtain and characterize, for the first time in bovine species, presumptive MSCs from the epithelial portion of the amnion (AECs) and from the AF (AF-MSCs) to be used for clinical applications. AECs display a polygonal morphology, whereas AF-MSCs exhibit a fibroblastic-like morphology only starting from the second passage, being heterogeneous during the primary culture. For both lines, the proliferative ability has been found constant over the ten passages studied and AECs show a statistically lower (P!0.05) doubling time with respect to AF-MSCs. AECs express MSC-specific markers (ITGB1 (CD29), CD44, ALCAM (CD166), ENG (CD105), and NT5E (CD73)) from P1 to P3; in AF-MSCs, only ITGB1, CD44, and ALCAM mRNAs are detected; NT5E is expressed from P2 and ENG has not been found at any passage. AF-MSCs and AECs are positive for the pluripotent markers (POU5F1 (OCT4) and MYC (c-Myc)) and lack of the hematopoietic markers. When appropriately induced, both cell lines are capable of differentiating into ectodermal and mesodermal lineages. This study contributes to reinforce the emerging importance of these cells as ideal tools in veterinary medicine. A deeper evaluation of the immunological properties needs to be performed in order to better understand their role in cellular therapy.

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“…AM has been shown to act as a scaffold for cell growth [17], promote epithelial wound healing [18] and to exert anti-inflammatory [19], anti-angiogenic [20], anti-fibrotic [21] and anti-microbial effects [22]. These mechanisms are, in part, attributed to a wide range of biological factors present in AM, for example epidermal growth factor (EGF) and transforming growth factor (TGF)-b1 [23].…”

Section: Introductionmentioning

confidence: 99%

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Allen¹,

Clare²,

Stewart³

et al. 2013

Acta Ophthalmologica

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Purpose: Dried amniotic membrane (AM) can be a useful therapeutic adjunct in ophthalmic surgery and possesses logistical advantages over cryopreserved AM. Differences in preservation techniques can significantly influence the biochemical composition and physical properties of AM, potentially affecting clinical efficacy. This study was established to investigate the biochemical and structural effects of drying AM in the absence and presence of saccharide lyoprotectants and its biocompatibility compared to cryopreserved material.Methods: AM was cryopreserved or dried with and without pre-treatment with trehalose or raffinose and the antioxidant epigallocatechin (EGCG). Structural and visual comparisons were assessed using electron microscopy. Localisation, expression and release of AM biological factors were determined using immunoassays and immunofluorescence. The biocompatibility of the AM preparations co-cultured with corneal epithelial cell (CEC) or keratocyte monolayers were assessed using cell proliferation, cytotoxicity, apoptosis and migration assays.Results: Drying devitalised AM epithelium, but less than cryopreservation and cellular damage was reduced in dried AM pre-treated with trehalose or raffinose. Dried AM alone, and with trehalose or raffinose showed greater factor retention efficiencies and bioavailability compared to cryopreserved AM and demonstrated a more sustained biochemical factor time release in vitro. Cellular health assays showed that dried AM with trehalose or raffinose are compatible and superior substrates compared to cryopreserved AM for primary CEC expansion, with increased proliferation and reduced LDH and caspase-3 levels. This concept was supported by improved wound healing in an immortalised human CEC line (hiCEC) cocultured with dried and trehalose or raffinose membranes, compared to cryopreserved and fresh AM.Conclusions: Our modified preservation process and our resultant optimised dried AM has enhanced structural properties and biochemical stability and is a superior substrate to conventional cryopreserved AM. In addition this product is stable and easily transportable allowing it to be globally wide reaching for use in clinical and military sectors.

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Suppression of inflammatory and fibrotic responses in allergic inflammation by the amniotic membrane stromal matrix

Abstract: The AMM is capable of suppressing fibrotic responses in an in vitro system of effector stages of ocular allergic inflammation. These data may provide a basis for exploring matrix components in the AM for the treatment of allergic eye disease.

Cited by 72 publications

References 40 publications

Therapeutic Effect of Human Amniotic Membrane–Derived Cells on Experimental Arthritis and Other Inflammatory Disorders

Therapeutic Effect of Human Amniotic Membrane–Derived Cells on Experimental Arthritis and Other Inflammatory Disorders

Mesenchymal stem cells from amnion and amniotic fluid in the bovine

Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

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