Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Allen, Claire; Clare, G.; Stewart, Elizabeth A.; Branch, Matthew J.; McIntosh, Owen D.; Verma, Mahesh Kumar; Dua, Hs; Hopkinson, Andrew

doi:10.1111/j.1755-3768.2013.s002.x

Cited by 5 publications

(6 citation statements)

References 40 publications

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“…Okabe et al found that the structures of collagen fibers in the connective tissues were not destroyed by the hyperdry device and were more stable than cryopreserved AM [7]. Allen et al also showed that the biochemical composition of the dried AM, including the number of factors such as epidermal growth factor and TGF-β1, were similar to fresh AM [22]. Moreover, rabbit models have also shown that HD-AM can be at least as efficacious as cryopreserved AM when used as a substrate for ocular surface reconstruction [23].…”

Section: Discussionmentioning

confidence: 88%

Comparison of hyperdry amniotic membrane transplantation and conjunctival autografting for primary pterygium

Pan¹

2018

http://isrctn.com/

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Background: The purpose of this study was to evaluate the safety and effectiveness of the hyperdry amniotic membrane transplantation compared with conjunctival autografting for the treatment of primary pterygium. Methods: One hundred and forty-one eyes from 130 patients with primary pterygium were treated with excision followed by hyperdry amniotic membrane or conjunctival autografting after random selection. Seventy-nine eyes from 71 patients received hyperdry amniotic membrane transplantation (HD-AM group), and 62 eyes from 59 patients received conjunctival autografting (CG group). Patients were followed up at one week and one, three, six, and 12 months post-surgery. Recurrence rate, postoperative complications, and final follow-up patient visits were prospectively evaluated. Results: The mean follow-up duration was 12.56 ± 4.35 months in the HD-AM group and 12.85 ± 3.90 months in the CG group. Recurrences were detected in four eyes (5.06%) in the HD-AM group and 13 eyes (20.97%) in the CG group. A statistically significant difference in frequency of recurrence between the two groups (P = 0.003) was observed. The cumulative non-recurrence rates at six and 12 months in all patients stratified by age and sex were not significantly different (P = 0.642 and P = 0.451, respectively, by log-rank test). Graft retraction and necrosis were not detected in the two groups during the follow-up period. Conclusion: Hyperdry amniotic membrane transplantation was effective in preventing pterygium recurrence when compared with conjunctival autografting and can be considered a preferable and safe grafting procedure for primary pterygium. Trial registration: Current Controlled Trials ISRCTN16900270, Retrospectively registered (Date of registration: 3 May 2018).

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Section: Discussionmentioning

confidence: 88%

Comparison of hyperdry amniotic membrane transplantation and conjunctival autografting for primary pterygium

Pan¹

2018

http://isrctn.com/

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“…Described bio-objects can be used to produce stem cells [14,20], in an experiment, testing of pharmaceuticals [10], auto-banking [17], for laboratory studies [7,9]. Fetal membranes are used as scaffolds for constructions in tissue engineering [15,16], allografts in reconstructive surgery [11,18] and ophthalmology [13,6]. Several studies have considered the expediency of isolating cells not from the native, but from the frozen placental material [19], which greatly simplifies the technology of low-temperature banking, eliminating the need to isolate cells in a short time after the birth of the placenta.…”

Section: Institute For Problems Of Cryobiology and Cryomedicine Of Thmentioning

confidence: 99%

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Prokopіuk¹,

Prokopyuk²,

Мусатова³

et al. 2015

JCOT

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There have been studied morphological safety and functional state of the explants of human placenta, umbilical cord and fetal membranes by vital staining techniques using the MTT and resazurin reduction tests, level of glucose in incubation medium, activity of lactate dehydrogenase and alkaline phosphatase before and after cryopreservation. It has been found that proposed program of cryopreservation allows keeping a high level of viability of the explants of placenta, umbilical cord and fetal membranes, the most informative methods of assessing the safety of these biological objects before and after cryopreservation are method of vital staining, determination of glucose content in incubation medium, MTT-test and resazurin reduction test.

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“…7,8,9 The h AM has the additional advantage of abundant availability, and has been clinically used in other applications with success for over a century. 10-13 While a cell-mediated fusion of the layers has proven successful in preliminary studies, this study investigated the use of freeze-drying in conjunction with a fibrin sealant to bind adjacent layers. 6,14 Without the need for pre-population with autologous cells, the conduits would be readily available, easily transportable, and storable extended time periods.…”

Section: Introductionmentioning

confidence: 99%

Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model

Amensag

Goldberg

O’Malley

et al. 2017

Journal of Vascular Surgery

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Background Herein we describe a small-diameter vascular graft constructed from rolled human amniotic membrane (hAM), with in vitro evaluation and subsequent in vivo assessment of its mechanical and initial biological viability in the early post-implantation period. This approach for graft construction allows for customization of graft dimensions, with wide-ranging potential clinical applicability as a non-autologous, allogeneic, cell-free graft material. Methods and Results Acellular hAM were rolled into layered conduits (3.2-mm diameter) that were bound with fibrin and lyophilized. In vitro analysis Constructs were seeded with human smooth muscle cells (SMC) and cultured under controlled arterial hemodynamic conditions. SMC were shown to adhere to, proliferate within, and remodel the scaffold over a four-week culture period. At the end of the culture period, there was histologic and biomechanical evidence of graft wall layer coalescence. In vivo analysis The acellular hAM conduits were surgically implanted as arterial interposition grafts into the carotid arteries of immunocompetent rabbits. Grafts demonstrated patency over four weeks (n=3) with no hyperacute rejection or thrombotic occlusion. Explants displayed histologic evidence of active cellular remodeling, with endogenous cell repopulation of the graft wall concurrent with degradation of initial graft material. Cells were shown to align circumferentially to resemble a vascular medial layer. Conclusions The vascular grafts were shown to provide a supportive scaffold allowing for cellular infiltration and remodeling by host cell populations in vivo. Using this approach, “off-the-shelf” vascular grafts can be created with specified diameters and wall thicknesses to satisfy specific anatomical requirements in diverse patient populations.

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Augmented dried versus cryopreserved amniotic membrane as an ocular surface dressing

Cited by 5 publications

References 40 publications

Comparison of hyperdry amniotic membrane transplantation and conjunctival autografting for primary pterygium

Comparison of hyperdry amniotic membrane transplantation and conjunctival autografting for primary pterygium

Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Pilot assessment of a human extracellular matrix-based vascular graft in a rabbit model

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