Safety of placental, umbilical cord and fetal membrane explants after cryopreservation

Abstract

There have been studied morphological safety and functional state of the explants of human placenta, umbilical cord and fetal membranes by vital staining techniques using the MTT and resazurin reduction tests, level of glucose in incubation medium, activity of lactate dehydrogenase and alkaline phosphatase before and after cryopreservation. It has been found that proposed program of cryopreservation allows keeping a high level of viability of the explants of placenta, umbilical cord and fetal membranes, the most informative methods of assessing the safety of these biological objects before and after cryopreservation are method of vital staining, determination of glucose content in incubation medium, MTT-test and resazurin reduction test.

Placenta and Placental Derivatives in Regenerative Therapies: Experimental Studies, History, and Prospects

“…Active work with biological material requires appropriate biobanks and biobanking technologies. Long-term storage of placental components may be aimed at clinical application and scientific research, as well as development and testing of the novel drugs [ 3 , 120 – 123 ].…”

“…Methods of chemical preservation, high-temperature sterilization, hypothermic storage, low-temperature storage, and cryosublimation have been used for the storage of placental components. Selection of the storage method depends on the type of material and the purpose of its further application [ 5 , 120 , 122 , 124 ].…”

“…The most conventional method for storage of biological objects, which provides high levels of preservation for a long period of time, is cryopreservation [ 124 ]. Cord blood serum, placental extracts, cell suspensions, chorionic and amniotic membranes, and placental tissue are all suitable for cryopreservation procedures [ 120 , 123 ]. Possibility to isolate MSCs with a stable genome from cryopreserved placental tissues, similar to the population of cells isolated from the native (fresh) tissues, significantly extends the scopes of cryopreservation of the placental material [ 128 ].…”

“…Possibility to isolate MSCs with a stable genome from cryopreserved placental tissues, similar to the population of cells isolated from the native (fresh) tissues, significantly extends the scopes of cryopreservation of the placental material [ 128 ]. The placenta is a unique object for low-temperature biobanking and autobanking [ 6 , 120 , 122 ]. In most countries, the application of placenta does not face ethical issues and women positively evaluate this possibility, otherwise considering the material as a “waste.” Donation of the placenta is physiologically indifferent for the donor.…”