Suppression of <i>Streptococcus sobrinus</i> 6715 (g) in plaques by <i>Streptococcus mutans</i> 32K (c)

Ikeda, Tadashi; Kurita, T; Hirasawa, Masatomo

doi:10.1111/j.1600-0714.1988.tb01318.x

Cited by 7 publications

(4 citation statements)

References 19 publications

(28 reference statements)

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“…The beginning and development of caries are determined by a host-parasite relationship, which can be altered by several environmental factors as demonstrated by in vitro models simulating the in vivo phenomenon in the mouth (9,20). The in vitro studies of the bacterial relationship based on the action of these microorganisms in the plaque formation on tooth surfaces and their potential to induce caries are in agreement with several results reported in the literature (3,(8)(9)(10)14,15).…”

Section: Introductionsupporting

confidence: 78%

“…The samples were donated by the Microbiology Laboratory of Chiba University -Japan. A 0.2 mL sample from each culture was added to a tube containing 5 mL of BHI medium supplemented with 10% fermentable carbohydrate, either sucrose, glucose, fructose or glucose (5%) + fructose (5%), and incubated at 37ºC for 24 h in a 10% CO2 atmosphere (10). After 3, 6, 12 and 24 h, 0.1 mL of the mixture was submitted to decimal dilution from 10 -1 to 10 -4 in sterile saline (0.9% NaCl), and plated in BHI-agar medium with rifampicin (0.1 mg/mL) or streptomycin (1.0 mg/mL).…”

Section: Suppression Of Bacterial Plaque Formationmentioning

confidence: 99%

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In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus

Pereira¹,

Gonçalves²,

Höfling³

2006

Braz. J. Microbiol.

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The in vitro study of the interactions between S. mutans and S. sobrinus is important to determine the role of these microorganisms in the formation of biofilms on dental structures and their potential to induce carious lesions. The objective of this research was to study the suppression of bacterial plaque formation and its recolonization by rifampycin-resistant S.mutans and streptomycin-resistant S. sobrinus. To study the competitive relationship between these species, previously standardized strains were incubated in media containing different fermentable carbohydrates. At determined time intervals, samples were collected from mixed cultures of S. mutans and S. sobrinus, diluted and plated on BHI-agar containing rifampycin or streptomycin to determine the number of viable cells of each species by counting colonyforming units. In order to study the bacterial colonization process and in vitro recolonization of bacterial plaque, three experiments were performed: I -co-cultivation of S. mutans and S. sobrinus; II -inoculation of bacterial plaque pre formed by S. sobrinus with S. mutans; and III -bacterial plaque pre formed by S. mutans dispersed and plated on BHI-agar containing streptomycin or rifampicin to determine the number of viable cells for each species. The results indicated a predominance of S. mutans in relation to S. sobrinus, demonstrating the capacity of S. mutans to inhibit plaque formation by S. sobrinus and recolonize the surfaces.

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Section: Introductionsupporting

confidence: 78%

Section: Suppression Of Bacterial Plaque Formationmentioning

confidence: 99%

In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus

Pereira¹,

Gonçalves²,

Höfling³

2006

Braz. J. Microbiol.

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“…The preferential location of mutans streptococci below the contact area might be relat ed to the stagnation in this location (as in occlusal fis sures), the ability of such species to avoid antibacterial factors at this site or, more likely, to this site being less accessible to the buffering and substrate clearance effect of saliva. In such an environment, the pH may be lower for longer periods, and such conditions would favour the growth of mutans streptococci at the expense of less acidogenic and aciduric species Newman et al, 1976;Bradshaw et al, 1989], S. mutans is also more bacteriocinogenic than S. sobrinus [Lindquist and Emilson, 1991b], and growth of the latter species can be suppressed by bacteriocin production by S. mutans [Ikeda et al, 1988]. Alternatively, differences in the pat tern of colonisation by S. mutans and S. sobrinus might be related to the fact that they possess different abilities to metabolise locally available indigenous nutrients [Homer and Beighton, 1991].…”

Section: Discussionmentioning

confidence: 99%

Distribution of Streptococcus mutans and Streptococcus sobrinus at Sub-Sites in Human Approximal Dental Plaque

Ahmady

Marsh²,

Newman³

et al. 1993

Caries Res

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The distribution and prevalence of Streptococcus mutans and Streptococcus sobrinus were determined at three sub-sites in human approximal plaque: away from (A) to the side of (S) and below (B) the contact area. Small plaque samples were taken from all three sub-sites on clinically sound approximal surfaces of a single premolar from each of 21 schoolchildren. S. mutans was detected significantly more often and in higher proportions than S. sobrinus from sub-sites A (p = 0.019), S (p = 0.034) and B (p = 0.004). S. mutans was detected in highest proportions from the B site compared to the A site (p = 0.025); there were no significant differences in the isolation frequency or prevalence of S. sobrinus from any of the sub-sites. S. mutans and S. sobrinus were never isolated together from the A sub-sites and were recovered together most commonly from the B sub-sites (p < 0.01). It is concluded that S. mutans and S. sobrinus preferentially colonise the most caries-prone site apical to the contact area.

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“…The term BLIS has been adopted when referring to bacteriocin-like inhibitory effects before complete characterisation of the inhibitory molecule(s) [5]. The reported incidence of BLIS production among mutans streptococci has ranged from 11% to 100% [6][7][8][9][10][11][12][13][14], the differences in detection frequencies probably reflecting differences in the test conditions and in the BLIS indicator strains used.…”

Section: Introductionmentioning

confidence: 99%

Different bacteriocin activities of Streptococcus mutans reflect distinct phylogenetic lineages

Balakrishnan

Simmonds

Kilian

et al. 2002

Journal of Medical Microbiology

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Bacteriocins produced by mutans streptococci are known as mutacins. In this study 16 broadly active mutacin-producing Streptococcus mutans strains from New Zealand, North America and Europe were classified into four groups (A-D) on the basis of differences in their activity in deferred antagonism tests against either the homologous producer strain (to test for presence of self-immunity) or indicator strains Staphylococcus aureus 46 and Enterococcus faecium TE1. Two of the strains included in the study (UA140 and UA96) were representatives of the group I and II mutacin producer strains previously described by Caufield and co-workers. One of the New Zealand isolates of group A (S. mutans strain N) appeared to produce inhibitory activity similar to that of the group I prototype strain UA140. Four other New Zealand isolates of group B (S. mutans strains M19, M34, B34 and D14) had mutacin II-like activity. The group B mutacin producers differed from the group A mutacin producers in their additional activity against Staph. aureus 46. Seven S. mutans strains (M46, B46, B57, M12, M28, B28 and 13M) were distinguished from the group A and group B mutacin producers in that they inhibited E. faecium TE1. These were called group C mutacin producers. Strains H7 and H23 resembled the group C strains in their action on both indicator strains TE1 and 46. However, these two strains failed to exhibit immunity to their own inhibitory products in the deferred antagonism test and were separately classified as group D mutacin producers. Phylogenetic analysis of the strains by several genotypic and phenotypic characteristics revealed that the mutacin groups were associated with distinct evolutionary lineages of S. mutans.

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Suppression of Streptococcus sobrinus 6715 (g) in plaques by Streptococcus mutans 32K (c)

Cited by 7 publications

References 19 publications

In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus

In vitro bacterial plaque suppression and recolonization by S. mutans and S. sobrinus

Distribution of Streptococcus mutans and Streptococcus sobrinus at Sub-Sites in Human Approximal Dental Plaque

Different bacteriocin activities of Streptococcus mutans reflect distinct phylogenetic lineages

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