Suppression of human peripheral blood lymphocyte proliferation by immortalized mesenchymal stem cells derived from bone marrow of Banna Minipig inbred-line

Liu, J.; Xiao, Lu; Wan, Lin; Li, Y.P.; Li, S.F.; Zeng, Li; Zeng, Yuhan; Li, Cheng; Lu, Yanrong; Cheng, Jun

doi:10.1016/j.transproceed.2004.11.090

Cited by 49 publications

(42 citation statements)

References 8 publications

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“…To verify this assumption, numerous studies which showed that MSCs directly and strongly suppressed T-cell proliferation in vitro have been carried out. 13,[17][18][19] Data reported in our paper corroborates these findings by confirming the inhibitory effect of mitogen-induced MSCs on T-cell proliferation. Moreover, under our experimental conditions, this effect was dramatic and dose dependent.…”

Section: Discussionsupporting

confidence: 90%

“…11 A similar dispute also exists with regard to the role of IL-10. 11,18,24 The reason for this divergence is probably due to different manipulations in the experiments performed by different researchers, such as the time of introducing the antibody and the presence or absence of cell-cell contact. For instance, in the present study, MSCs were treated with antibody prior to co-culture with LBs, while in other studies, antibody was added directly to the co-culture system of LBs and MSCs.…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation

Gao

Chen

Wei

et al. 2008

Cell Biochemistry & Function

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We investigated the mechanism underlying the inhibitory effect of rat mesenchymal stem cells (MSCs) on non-specific mitogen-stimulated lymphocytes (LCs) and lymphoblasts (LBs). We used MSCs of passages 2-8 prepared from Sprague-Dawley (SD) rats. LCs were isolated from the spleens of SD rats. Mixed LCs reactions of mitomycin C-treated MSCs with concanavalin A (ConA)-stimulated LCs or LBs were performed, and the proliferation inhibition effect was tested by MTS assay. The cytotoxicity of MSCs against naïve and ConA-stimulated LBs was detected, after co-culturing for 24 h, by lactate dehydrogenase release assay. The rate of apoptosis of ConA-stimulated LBs was measured by flow cytometry after incubation with MSCs for 9 h in the ratio 10:1. The MSCs were treated with Fas ligand (FasL), transforming growth factor (TGF)-beta, and interleukin (IL)-10 blocking antibodies and co-cultured with ConA-stimulated LBs to observe the apoptosis and growth inhibitory effect. The main outcomes were bone marrow-derived adherent CD29+, CD44+, CD45-, CD54+, CD95+, and SH-2+ MSCs. FasL, TGF-beta, and IL-10 production by MSCs were visualized by immunocytochemical analysis. MSCs exhibited a dose-dependent growth inhibitory effect on ConA-stimulated LCs and LBs. When treated with anti-FasL and anti-IL-10 blocking antibodies, the inhibitory effect of MSCs on LBs proliferation, and the effect of apoptosis induction on LBs decreased. Anti-TGF-beta blocking antibody treatment did not significantly influence MSCs. Therefore, the inhibitory effects of MSCs against activated LBs were significantly stronger than that against naïve LCs. FasL and IL-10, rather than TGF-beta, play important roles in the immunosuppressive effects of MSCs.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation

Gao

Chen

Wei

et al. 2008

Cell Biochemistry & Function

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“…The measurement of T-cell proliferation was also performed using MTT method. Stimulation index (SI) values were calculated by the following formula: SI ¼ proliferation of stimulated lymphocyte with or without SCAP/proliferation of unstimulated lymphocyte alone (Liu et al, 2004).…”

Section: Swine Modelmentioning

confidence: 99%

Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla

Ding

Wang

Liu

et al. 2010

Journal Cellular Physiology

107

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Stem cells from apical papilla (SCAP) are a novel population of multipotent stem cells that, although similar to dental pulp stem cells, are a discrete source of dental stem cells. SCAP have potential roles in root development, apexogenesis, pulp/dentin regeneration, and bioroot engineering. However, procedures to store and preserve SCAP for future clinical applications have not been explored. In this study, we compared human freshly isolated SCAP (fSCAP) with cryopreserved SCAP (cSCAP) in terms of cell viability, colony-forming efficiency, cell proliferation rate, multilineage differentiation potential, profiles of mesenchymal stem cell (MSC) markers, karyotype analysis, and immunological assays. cSCAP showed a similar viable cell ratio, colony-forming efficiency, cell proliferation rate, multilineage differentiation potential, MSC surface markers, apoptotic rate, and G-banded karyotype when compared to fSCAP. There was no significant difference between fSCAP and cSCAP with regard to immune properties. In addition, cSCAP of miniature pig possessed the similar proliferation rate, differentiation potential, and immunomodulatory function as seen in fSCAP. This study demonstrates that cryopreservation does not affect the biological and immunological properties of SCAP, supporting the feasibility of SCAP cryopreservation in nitrogen.

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“…Gene transfer of tolerogenic molecules such as Fas ligand (FasL) and haemoxygenase-1 (HO-1) may offer protection against rejection. These cytoprotective molecules have recently been implicated in MSC-mediated downregulation of T cell responses (Liu et al 2004), as well as in enhanced MSC survival following their transplantation in infarcted rat hearts resulting in improved cardiac function (Zhang et al 2005b). FasL is a type II cell surface protein which has multiple roles (Askenasy et al 2005).…”

Section: Strategies To Induce Tolerance To Overcome Rejection Of Allomentioning

confidence: 99%

“…Perhaps genetic modification of MSCs to express FasL may induce peripheral allograft tolerance by selectively deleting alloreactive T cells. Recent data from Liu et al (2004) lymphocyte proliferation by MSCs. The consequence of FasL expression on MSCs will therefore be an important immunomodulatory pathway to consider for producing an 'off-the-shelf' tissue engineered organ.…”

Section: Strategies To Induce Tolerance To Overcome Rejection Of Allomentioning

confidence: 99%

Immune response to stem cells and strategies to induce tolerance

Batten

Rosenthal

Yacoub

2007

Phil. Trans. R. Soc. B

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Although recent progress in cardiovascular tissue engineering has generated great expectations for the exploitation of stem cells to restore cardiac form and function, the prospects of a common massproduced cell resource for clinically viable engineered tissues and organs remain problematic. The refinement of stem cell culture protocols to increase induction of the cardiomyocyte phenotype and the assembly of transplantable vascularized tissue are areas of intense current research, but the problem of immune rejection of heterologous cell type poses perhaps the most significant hurdle to overcome. This article focuses on the potential advantages and problems encountered with various stem cell sources for reconstruction of the damaged or failing myocardium or heart valves and also discusses the need for integrating advances in developmental and stem cell biology, immunology and tissue engineering to achieve the full potential of cardiac tissue engineering. The ultimate goal is to produce 'off-the-shelf ' cells and tissues capable of inducing specific immune tolerance.

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Suppression of human peripheral blood lymphocyte proliferation by immortalized mesenchymal stem cells derived from bone marrow of Banna Minipig inbred-line

Cited by 49 publications

References 8 publications

Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation

Inhibitory effect of mesenchymal stem cells on lymphocyte proliferation

Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla

Immune response to stem cells and strategies to induce tolerance

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