Effect of cryopreservation on biological and immunological properties of stem cells from apical papilla

Abstract

Stem cells from apical papilla (SCAP) are a novel population of multipotent stem cells that, although similar to dental pulp stem cells, are a discrete source of dental stem cells. SCAP have potential roles in root development, apexogenesis, pulp/dentin regeneration, and bioroot engineering. However, procedures to store and preserve SCAP for future clinical applications have not been explored. In this study, we compared human freshly isolated SCAP (fSCAP) with cryopreserved SCAP (cSCAP) in terms of cell viability, colony-forming efficiency, cell proliferation rate, multilineage differentiation potential, profiles of mesenchymal stem cell (MSC) markers, karyotype analysis, and immunological assays. cSCAP showed a similar viable cell ratio, colony-forming efficiency, cell proliferation rate, multilineage differentiation potential, MSC surface markers, apoptotic rate, and G-banded karyotype when compared to fSCAP. There was no significant difference between fSCAP and cSCAP with regard to immune properties. In addition, cSCAP of miniature pig possessed the similar proliferation rate, differentiation potential, and immunomodulatory function as seen in fSCAP. This study demonstrates that cryopreservation does not affect the biological and immunological properties of SCAP, supporting the feasibility of SCAP cryopreservation in nitrogen.

LBH589 Promotes Osteogenic and Dentinogenic Differentiation of Stem Cells from the Apical Papilla by Inhibiting Histone Deacetylation

“…STRO-1, CD90 (Thy-1), and CD146 were expressed in SCAPs according to flow cytometric analysis, and SCAPs showed colony-forming properties and osteoblast and adipose differentiation potential, as reported previously (data not shown) 3) .…”

“…Recently, mesenchymal stem cells were isolated from dental and craniofacial tissues [1][2][3] . These post-natal stem cells shared similar biological characteristics with bone marrow stem cells and were shown to play a significant role in tissue regeneration and cell therapy [4][5] .…”

Multipotent Dental Stem Cells: An Alternative Adult Derived Stem Cell Source for Regenerative Medicine

“…The potential use of dental stem cells has become even more viable for tissue regeneration and other therapies with the recent advances in cryopreservation. These advances allow proliferation and long term storage of these cells for future cell therapy treatments while maintaining their differentiation potential (Ding et al, 2010;Papaccio et al, 2006;Seo et al, 2005;Woods et al, 2009;Zhang et al, 2006).…”

Importância Dos Tecidos Dentais E Periodontais Como Fontes De Células-Tronco

“…Representa um tecido localizado no exterior do forame apical e existe enquanto o ápice dentário ainda encontrase aberto durante o processo de desenvolvimento radicular até a completa erupção do dente na cavidade oral (DING et al, 2010).…”

“…O tecido apical é fracamente ligado ao ápice da raiz em desenvolvimento e pode ser facilmente retirado com o auxílio de uma pinça ou cureta (DING et al, 2010). No entanto, como se trata de um tecido embrionário, que se torna também polpa dental, suas células-tronco podem ser isoladas apenas em um determinado período do desenvolvimento dentário, enquanto a raiz ainda está aberta .…”