Suppression of genotoxicity of carcinogens by (−)-epigallocatechin gallate

Hayatsu, Hikoya; Inada, Naomi; Kakutani, Toshifumi; Arimoto, Sakae; Negishi, Tomoe; Mori, Kazuko; Okuda, Takuo; Sakata, Isao

doi:10.1016/0091-7435(92)90044-i

Cited by 86 publications

(30 citation statements)

References 12 publications

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“…4C. Several studies reported that the chemopreventive agents such as EGCG, N-(4-hydroxyphenyl)retinamide, and curcumin are not genotoxic (33,34) and selectively inhibit cancer cells, but not normal cell lines (35)(36)(37). For example, EGCG inhibited the growth of A-375 and Hs-294T melanoma cells, but did not affect the growth of normal human epidermal melanocytes (37).…”

Section: Discussionmentioning

confidence: 99%

New Role of (−)-Epicatechin in Enhancing the Induction of Growth Inhibition and Apoptosis in Human Lung Cancer Cells by Curcumin

Saha

Kuzuhara

Echigo

et al. 2010

Cancer Prevention Research

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Curcumin, a phenolic compound isolated from the plant Curcuma longa (Linn), is ingested every day in the Indian subcontinent and is well reported to possess cancer-preventive activity. To achieve effective cancer prevention with curcumin, we need to find a new method to enhance the effects of curcumin in the diet. Based on our evidence that (−)-epicatechin (EC), an inert catechin, enhances the cancer-preventive activity of green tea catechins, we studied the enhancing effects of EC on inductions of growth inhibition and apoptosis in human lung cancer cell lines PC-9 and A549 with curcumin. The combination of curcumin with EC significantly increased the inhibition of cell growth compared with curcumin or EC alone. The combination similarly increased both apoptosis and expression of GADD153 and GADD45 genes, associated with their enhanced protein production. Knockdown of GADD153 or GADD45 by small interfering RNA abrogated the apoptosis induction and growth inhibition induced by the combination, indicating the crucial role of their upregulation. Treatments of PC-9 cells with c-Jun-NH 2 -kinase inhibitor SP600125, with p38 mitogen-activated protein kinase inhibitor SB202190 and with PD98059 (extracellular signalregulated kinase 1/2 inhibitor) all increased the upregulation of GADD153 and GADD45 genes by the combination. Because EC was previously shown to enhance the incorporation of EGCG into PC-9 cells, we think that EC has similar effects on curcumin. This report is the first report on the enhancing effects of EC on curcumin, and the data suggest that EC plays a significant role in the enhancement of the cancerpreventive activity of curcumin in the diet. Cancer Prev Res; 3(8); 953-62. ©2010 AACR.

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Section: Discussionmentioning

confidence: 99%

New Role of (−)-Epicatechin in Enhancing the Induction of Growth Inhibition and Apoptosis in Human Lung Cancer Cells by Curcumin

Saha

Kuzuhara

Echigo

et al. 2010

Cancer Prevention Research

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“…EGCE was effective in reducing the mutagenecity of Trp-p-2(NHOH) in mouse FM3A cells in culture. EGCE was also effective in inhibiting DNA single strand breaks in vitro caused by Glu-p-1(NHOH) [160].…”

Section: B In Vitro Studiesmentioning

confidence: 98%

“…A study performed by [148] reported that EGCG suppressed the direct-acting mutagenicity of 3-hydroxyamino-1-methyl-5H-pyrido-(4,3-b) indole (Trp-p-2(NHOH)) and 2-hydroxyamino-6-methyldipyrido(1,2-a:3,2-d) imidazole (Glu-p-1(NHOH)) in the Ames salmonella test. furthermore, they added that EGCE has also a suppressive effect in the in vivo Drosophila mutation assays, i.e., the wing spot test, and the DNA repair test, on several carcinogens.…”

Section: A In Vivo Studiesmentioning

confidence: 99%

Cancer Chemoprevention by Dietary Polyphenols

Aly¹,

Mahmoud²

2013

Carcinogenesis

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“…The heat sterilization conditions for different beverages are determined based on certain characteristics of the beverage, including pH and packaging; thus, the relative epimerization of catechins is greater in certain beverages. Regarding the genotoxic aspects of tea catechins, many studies have demons-trated that tea catechins could suppress the genotoxic activity of various carcinogens with both in vitro and in vivo systems [16][17][18][19][20][21][22][23]. As to the genotoxic profile of tea catechins when tested alone, Chang et al [24] have shown that there is minimal genotoxic concern with a decaffeinated green tea catechin mixture (Polyphenon E) that contains about 50% epigallocatechin gallate and 30% other catechins.…”

Section: Introductionmentioning

confidence: 99%

Protective Effect of Green Tea Against Dimethylnitrosamine Induced Genotoxicity in Mice Bone Marrow Cells~!2010-01-11~!2010-03-29~!2010-08-27~!

Al-Fify¹,

Aly²

2010

TOCJ

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Cancer is a major cause of death in the world; thus, the prevention of this disease would have a significant impact on public health. Chemoprevention is defined as natural and synthetic pharmacological (chemical) agent to disrupt the process of carcinogenesis. Green tea is a potent chemopreventive agent in many test systems and has been shown to inhibit tumor promotion and induce apoptosis. It is one of the most frequently consumed beverages in the world. Green tea polyphenolics have demonstrated antimutagenic, anticarcinogenic, antioxidant and antipromotional effects.The aim of this work is to study the protective effect of green tea extract against genotoxic damage of dimethylnitrosamine (DMN) in mice bone marrow cells.Our results demonstrated that the administration of green tea extract 24 hr before the DMN injection significantly suppressed DMN-induced chromosomal aberrations and sister chromatid exchanges. The suppression was observed 18 hr, 24 hr and 48 hr after the DMN treatment but no suppressive effect was observed at the early period (6 hr and 12 hr) after the DMN treatment. Furthermore, the suppression was observed in all doses of DMN (4, 5 and 6 mg/kg) investigated. Mice were given green tea 2 hr before the DMN injection displayed no suppressive effect.Mice were given 2% green tea extract as the sole source of drinking water for four days before sacrifice displayed significantly suppressed DMN-induced chromosomal aberrations and sister chromatid exchanges.We conclude that green tea presents significant antigenotoxic concern under the anticipated conditions of use. These results are consistent with other antigenotoxicity studies of green tea.

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Suppression of genotoxicity of carcinogens by (−)-epigallocatechin gallate

Cited by 86 publications

References 12 publications

New Role of (−)-Epicatechin in Enhancing the Induction of Growth Inhibition and Apoptosis in Human Lung Cancer Cells by Curcumin

New Role of (−)-Epicatechin in Enhancing the Induction of Growth Inhibition and Apoptosis in Human Lung Cancer Cells by Curcumin

Cancer Chemoprevention by Dietary Polyphenols

Protective Effect of Green Tea Against Dimethylnitrosamine Induced Genotoxicity in Mice Bone Marrow Cells~!2010-01-11~!2010-03-29~!2010-08-27~!

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