Suppression of gene silencing: a threat to virus-resistant transgenic plants?

Mitter, Neena; Sulistyowati, Emy; Graham, Michael W.; Dietzgen, Ralf G.

doi:10.1016/s1360-1385(01)01947-1

Cited by 28 publications

(17 citation statements)

References 9 publications

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“…It was concluded that RNA-silencing mediated resistance in transgenic plants against viruses may be suppressed by infection of the plants with heterologous viruses that encode suppressors of gene silencing (Savenkov & Valkonen, 2001). Not equally definite was the outcome from the studies of Mitter et al (2001;2003) which showed that in transgenic tobacco plants, infection with Cucumber mosaic virus (CMV, genus Cucumovirus) expressing the silencing suppressor 2b protein could transiently suppress the silencing mediated immunity to PVY but solely in new leaves that emerged after CMV inoculation and for a limited period of time. The experiments were carried out for six months and different time intervals were examined between the two virus inoculations.…”

Section: Heterologous Virusesmentioning

confidence: 99%

Stability of Transgenic Resistance Against Plant Viruses

Vassilakos¹

2012

Transgenic Plants - Advances and Limitations

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Section: Heterologous Virusesmentioning

confidence: 99%

Stability of Transgenic Resistance Against Plant Viruses

Vassilakos¹

2012

Transgenic Plants - Advances and Limitations

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“…The stability of RNA silencing-based resistance in transgenic plants is frequently challenged by the suppressor factors expressed by plant viruses (11). To investigate the stability of the transgene-mediated silencing and its consequent viral resistance, two unrelated viruses of PVX (potexvirus) and TMV (tobamovirus) were chosen to challenge M11 plants.…”

Section: Rna Silencing Was Differently Suppressed By Pvx and Tmv Infementioning

confidence: 99%

RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants

Hou²,

Wang³

et al. 2008

BMB Reports

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The discovery of RNA silencing inhibition by virus encoded suppressors or low temperature leads to concerns about the stability of transgenic resistance. RNA-dependent RNA polymerase (RdRp) has been previously characterized to be essential for transgene-mediated RNA silencing. Here we showed that low temperature led to the inhibition of RNA silencing, the loss of viral resistance and the reduced expression of host RdRp homolog (

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“…This strategy functions through RNA silencing direct targeting on the viral genome (Grumet et al, 1987;Baulcombe, 2004;Wang and Metzlaff, 2005;Wang et al, 2006). This type of viral resistance can be overcome in two scenarios: in mixed infections by a strong silencing suppressor from unrelated viruses (Mitter et al, 2001) and through random sequence mutation during virus replication that lacks a proofreading mechanism (Kang et al, 2005b). Thus, our study also provides an immediate interest for agricultural studies and could potentially serve as a feasible solution to viral diseases in crops.…”

Section: Discussionmentioning

confidence: 98%

A Host RNA Helicase-Like Protein, AtRH8, Interacts with the Potyviral Genome-Linked Protein, VPg, Associates with the Virus Accumulation Complex, and Is Essential for Infection

et al. 2009

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The viral genome-linked protein, VPg, of potyviruses is a multifunctional protein involved in viral genome translation and replication. Previous studies have shown that both eukaryotic translation initiation factor 4E (eIF4E) and eIF4G or their respective isoforms from the eIF4F complex, which modulates the initiation of protein translation, selectively interact with VPg and are required for potyvirus infection. Here, we report the identification of two DEAD-box RNA helicase-like proteins, PpDDXL and AtRH8 from peach (Prunus persica) and Arabidopsis (Arabidopsis thaliana), respectively, both interacting with VPg. We show that AtRH8 is dispensable for plant growth and development but necessary for potyvirus infection. In potyvirus-infected Nicotiana benthamiana leaf tissues, AtRH8 colocalizes with the chloroplast-bound virus accumulation vesicles, suggesting a possible role of AtRH8 in viral genome translation and replication. Deletion analyses of AtRH8 have identified the VPg-binding region. Comparison of this region and the corresponding region of PpDDXL suggests that they are highly conserved and share the same secondary structure. Moreover, overexpression of the VPg-binding region from either AtRH8 or PpDDXL suppresses potyvirus accumulation in infected N. benthamiana leaf tissues. Taken together, these data demonstrate that AtRH8, interacting with VPg, is a host factor required for the potyvirus infection process and that both AtRH8 and PpDDXL may be manipulated for the development of genetic resistance against potyvirus infections.

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Suppression of gene silencing: a threat to virus-resistant transgenic plants?

Cited by 28 publications

References 9 publications

Stability of Transgenic Resistance Against Plant Viruses

Stability of Transgenic Resistance Against Plant Viruses

RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants

A Host RNA Helicase-Like Protein, AtRH8, Interacts with the Potyviral Genome-Linked Protein, VPg, Associates with the Virus Accumulation Complex, and Is Essential for Infection

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