RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants

Wu, Xiaoliang; Hou, Wen-Cui; Wang, Meimei; Zhu, Xitong; Li, Fang; Zhang, Jiedao; Li, Xinzheng; Guo, Xingqi

doi:10.5483/bmbrep.2008.41.5.376

Cited by 8 publications

(7 citation statements)

References 20 publications

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“…The marked decrease in siRNA level caused by low temperature is probably because of inhibition in enzymatic activity and/or gene expression of the protein(s) that plays important roles in siRNA generation and/or amplification. In transgenic tobacco, expression of a gene encoding RDR is down-regulated at low temperature (Wu et al 2008). Unlike TM, in the highly CD-tolerant cultivar TR, accumulation of CHS siRNAs is weakly inhibited by low temperature, and consequently CHS siRNAs are accumulated in the seed coat.…”

Section: Full and Partial Seed Coat Pigmentation In Yellow Soybeanmentioning

confidence: 99%

Suppressive mechanism of seed coat pigmentation in yellow soybean

et al. 2012

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In soybean seeds, numerous variations in colors and pigmentation patterns exist, most of which are observed in the seed coat. Patterns of seed coat pigmentation are determined by four alleles (I, ii, ik and i) of the classically defined I locus, which controls the spatial distribution of anthocyanins and proanthocyanidins in the seed coat. Most commercial soybean cultivars produce yellow seeds with yellow cotyledons and nonpigmented seed coats, which are important traits of high-quality seeds. Plants carrying the I or ii allele show complete inhibition of pigmentation in the seed coat or pigmentation only in the hilum, respectively, resulting in a yellow seed phenotype. Classical genetic analyses of the I locus were performed in the 1920s and 1930s but, until recently, the molecular mechanism by which the I locus regulated seed coat pigmentation remained unclear. In this review, we provide an overview of the molecular suppressive mechanism of seed coat pigmentation in yellow soybean, with the main focus on the effect of the I allele. In addition, we discuss seed coat pigmentation phenomena in yellow soybean and their relationship to inhibition of I allele action.

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Section: Full and Partial Seed Coat Pigmentation In Yellow Soybeanmentioning

confidence: 99%

Suppressive mechanism of seed coat pigmentation in yellow soybean

et al. 2012

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“…Overall, the experiments indicate that ihp‐PTGS is able to confer broad‐spectrum resistance/immunity to PPV even at low temperature. Conversely, RNA silencing‐mediated virus resistance driven by aberrant viral sense gene constructs (S‐PTGS) was lost when transgenic plants were challenged at 15 °C (Szittya et al ., ; Wu et al ., ). Our results are in accord with and extend the recent findings of Hu et al .…”

Section: Resistance Analysis Of H‐utr/p1 68 Transgenic Plants Challenmentioning

confidence: 97%

“…In particular, the RNA silencing driven by the expression of intron-hairpin (ihp) constructs (ihp-PTGS) has been shown to consistently confer virus resistance in most T0 plants (Di Nicola-Negri et al, 2005;Smith et al, 2000). Nevertheless, it has been reported that some abiotic and biotic conditions, such as low plant growth temperatures (Szittya et al, 2003;Wu et al, 2008) and mixed viral infections, can break RNA silencing-mediated virus resistance (Di Serio et al, 2002;Hassani-Mehraban et al, 2009;Jørgensen and Albrechtsen, 2007;Mitter et al, 2003;Savenkov and Valkonen, 2001;Simon-Mateo et al, 2003), thus representing a possible limitation for the full applicability of this biotechnological strategy.…”

mentioning

confidence: 99%

Robust RNA silencing‐mediated resistance to Plum pox virus under variable abiotic and biotic conditions

Nicola-Negri

Tavazza

Lucioli

et al. 2014

Molecular Plant Pathology

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Some abiotic and biotic conditions are known to have a negative impact on post-transcriptional gene silencing (PTGS), thus representing a potential concern for the production of stable engineered virus resistance traits. However, depending on the strategy followed to achieve PTGS of the transgene, different responses to external conditions can be expected. In the present study, we utilized the Nicotiana benthamiana–Plum pox virus (PPV) pathosystem to evaluate in detail the stability of intron-hairpin(ihp)-mediated virus resistance under conditions known to adversely affect PTGS. The ihp plants grown at low or high temperatures were fully resistant to multiple PPV challenges, different PPV inoculum concentrations and even to a PPV isolate differing from the ihp construct by more than 28% at the nucleotide level. In addition, infections of ihp plants with viruses belonging to Cucumovirus, Potyvirus or Tombusvirus, all known to affect PTGS at different steps, were not able to defeat PPV resistance. Low temperatures did not affect the accumulation of transgenic small interfering RNAs (siRNAs), whereas a clear increase in the amount of siRNAs was observed during infections sustained by Cucumber mosaic virus and Potato virus Y. Our results show that the above stress factors do not represent an important concern for the production,through ihp-PTGS technology, of transgenic plants having robust virus resistance traits.

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“…It is mediated by a large network of interacting effector molecules, and connected to several parallel regulatory and signaling pathways. It affects, both directly and indirectly, cellular processes varying from the cell cycle regulation and chromatin structure maintenance to transcriptional and posttranscriptional regulation, to stress and hormonal signaling, and to developmental differentiation [1-5]. Thus, RNA silencing plays a central role in manifestation of the genetic information of different eukaryotes.…”

Section: Introductionmentioning

confidence: 99%

Organ-specific alterations in tobacco transcriptome caused by the PVX-derived P25 silencing suppressor transgene

2013

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BackgroundRNA silencing affects a broad range of regulatory processes in all eukaryotes ranging from chromatin structure maintenance to transcriptional and translational regulation and longevity of the mRNAs. Particularly in plants, it functions as the major defense mechanism against viruses. To counter-act this defense, plant viruses produce suppressors of RNA silencing (Viral suppressors of RNA silencing, VSRSs), which are essential for viruses to invade their specific host plants. Interactions of these VSRSs with the hosts’ silencing pathways, and their direct and indirect interference with different cellular regulatory networks constitute one of the main lines of the molecular virus-host interactions. Here we have used a microarray approach to study the effects of the Potato virus X Potexvirus (PVX)-specific P25 VSRS protein on the transcript profile of tobacco plants, when expressed as a transgene in these plants.ResultsThe expression of the PVX-specific P25 silencing suppressor in transgenic tobacco plants caused significant up-regulation of 1350 transcripts, but down-regulation of only five transcripts in the leaves, and up- and down-regulation of 51 and 13 transcripts, respectively, in the flowers of these plants, as compared to the wild type control plants. Most of the changes occurred in the transcripts related to biotic and abiotic stresses, transcription regulation, signaling, metabolic pathways and cell wall modifications, and many of them appeared to be induced through up-regulation of the signaling pathways regulated by ethylene, jasmonic acid and salicylic acid. Correlations of these alterations with the protein profile and related biological functions were analyzed. Surprisingly, they did not cause significant alterations in the protein profile, and caused only very mild alteration in the phenotype of the P25-expressing transgenic plants.ConclusionExpression of the PVX-specific P25 VSRS protein causes major alterations in the transcriptome of the leaves of transgenic tobacco plants, but very little of any effects in the young flowers of the same plants. The fairly stable protein profile in the leaves and lack of any major changes in the plant phenotype indicate that the complicated interplay and interactions between different regulatory levels are able to maintain homeostasis in the plants.

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RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants

Cited by 8 publications

References 20 publications

Suppressive mechanism of seed coat pigmentation in yellow soybean

Suppressive mechanism of seed coat pigmentation in yellow soybean

Robust RNA silencing‐mediated resistance to Plum pox virus under variable abiotic and biotic conditions

Organ-specific alterations in tobacco transcriptome caused by the PVX-derived P25 silencing suppressor transgene

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