Supported membranes on soft polymer cushions: fabrication, characterization and applications

Sackmann, E.; Tanaka, Motomu

doi:10.1016/s0167-7799(99)01412-2

Cited by 394 publications

(352 citation statements)

References 27 publications

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“…[1][2][3][4][5] Phosphocholine (PC) lipids deposited on silica (SiO2) is the most commonly used system since PC liposomes readily fuse with silica under physiological conditions. [6][7][8][9][10][11] In addition, PC liposomes are zwitterionic and highly biocompatible.…”

Section: Introductionmentioning

confidence: 99%

A Stable Lipid/TiO₂ Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO₂

Wang

Liu

2015

J. Am. Chem. Soc.

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Zwitterionic phosphocholine (PC) lipids are highly biocompatible, representing a major component of the cell membrane. A simple mixing of PC liposomes and silica (SiO2) surface results in liposome fusion with the surface and formation of supported lipid bilayers. However, the stability of this bilayer is relatively low since adsorption is based mainly on weak van der Waals force. PC lipids strongly adsorb by TiO2 via chemical bonding between the lipid phosphate. The lack of fusion on TiO2 is attributable to the steric effect from the choline group in PC. In this study, inverse-phosphocholine lipids (CP) are used, directly exposing the phosphate.Using a calcein leakage assay and cryo-TEM, fusion of CP liposome with TiO2 is demonstrated.The stability of this supported bilayer is significantly higher than that of the PC/SiO2 system as indicated by washing the membrane under harsh conditions. Adsorption of CP liposomes by TiO2 is inhibited at high pH. Interestingly, the CP liposome cannot fuse with silica surface due to a strong charge repulsion. This study demonstrates an interesting interplay between soft matter surface and metal oxides. By tuning the lipid structure, it is possible to rationally control the interaction force. This study provides an alternative system for forming stable supported bilayers on TiO2, and represents the first example of interfacing inverse lipids with inorganic surfaces.

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Section: Introductionmentioning

confidence: 99%

A Stable Lipid/TiO₂ Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO₂

Wang

Liu

2015

J. Am. Chem. Soc.

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“…For instance, the Langmuir-Blodgett deposition allows the deposition of asymmetric bilayers, 22 dip-pen technology facilitates the fabrication of biomolecular arrays, 8 spin coating enables the quick generation of highly oriented, homogeneous bilayer stacks with defined thickness, 20 and evaporation induced self-assembly permits the creation of patterned nanocomposites of dissimilar materials. 21 In some applications, exposure of the sample to air should be avoided, and solution-based deposition of supported bilayers is preferred; 22 such procedures would also be suitable for a wider class of surfaces, including hydrophobic surfaces 23 or, in principle, also solid particles such as silica or glass beads. 24,25 Tiberg et al, 26 for example, reported a simple method of this kind for preparing model lipid bilayers by coadsorption with a nonionic surfactant.…”

mentioning

confidence: 99%

Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

Hohner¹,

David²,

Rädler³

2010

Biointerphases

114

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“…The patterning strategies include microfluidics 83,84 , photolithography 81,85 , electron beam lithography, microcontact printing 86 , nanoimprinting 82 , dip-pen nanolithography 87 and block-copolymer micelle nanolithography 88 . Some of these patterning strategies can be combined with supported fluid lipid bilayers on the same surface, providing both mobile and immobile stimuli 17,57,75,81,[89][90][91] . The cell biological application of these techniques was originally pioneered for the study of cell adhesion and focal adhesion sites 80,[92][93][94] .…”

Section: Direct Spatial Pattern On Solid Surfacesmentioning

confidence: 99%

Spatial organization and signal transduction at intercellular junctions

Manz

Groves

2010

Nat Rev Mol Cell Biol

117

113

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The coordinated organization of cell membrane receptors into diverse micrometre-scale spatial patterns is emerging as an important theme of intercellular signalling, as exemplified by immunological synapses. Key characteristics of these patterns are that they transcend direct protein-protein interactions, emerge transiently and modulate signal transduction. Such cooperativity over multiple length scales presents new and intriguing challenges for the study and ultimate understanding of cellular signalling. As a result, new experimental strategies have emerged to manipulate the spatial organization of molecules inside living cells. The resulting spatial mutations yield insights into the interweaving of the spatial, mechanical and chemical aspects of intercellular signalling.Cell-to-cell communication is mediated by various methods. Endocrine signals are secreted and reach distant target cells, paracrine signals are secreted and reach targets in the vicinity, and autocrine signals are secreted and received by the same cell. By contrast, in juxtacrine signalling, surfaces of interacting cells come into direct contact and receptor-ligand recognition at this interface triggers intracellular signalling. Cell-cell interactions involve multiple adhesion and signalling molecules, the collective behaviour of which regulates signal transduction 1 . Also intrinsic to juxtacrine signalling configurations are large physical constraints on molecular movement and assembly. Genetic and biochemical approaches have been invaluable in identifying the molecular components of signal transduction pathways in juxtacrine signalling and in characterizing the biochemical interactions among them. Despite this wealth of information, in many cases it remains impossible to describe the behaviour of a signalling system in terms of the individual molecular properties of its components. Protein-protein inter actions and the formation of molecular clusters are widely implicated in signal transduction and contribute to a first level of cooperativity [2][3][4] . Recently, the coordinated organization of cell membrane receptors into micrometre-scale patterns has emerged as a broadly important theme of intercellular signalling 1,[5][6][7][8][9] .A paradigm for the interplay of spatial patterns and signal transduction is the junction between T cells and their target cells, termed the immunological synapse [8][9][10][11][12][13] . Spatial HHMI Author Manuscript HHMI Author Manuscript HHMI Author Manuscriptpatterns of proteins at the cell-cell interface develop as hundreds of receptors recognize their cognate ligands on the apposed cell membrane. Multiple signalling and adhesion molecules also become organized into distinctive spatial patterns at the interface between the two cells 8,9,13,14 (FIG. 1). The patterns create long-range interactions and seem to have specific purposes in signal transduction 8,9 . They host the local enrichment or depletion of key signalling components, which can bias biochemical cascades towards different functional o...

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Supported membranes on soft polymer cushions: fabrication, characterization and applications

Cited by 394 publications

References 27 publications

A Stable Lipid/TiO₂ Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO₂

A Stable Lipid/TiO₂ Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO₂

Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

Spatial organization and signal transduction at intercellular junctions

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Supported membranes on soft polymer cushions: fabrication, characterization and applications

Cited by 394 publications

References 27 publications

A Stable Lipid/TiO2 Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO2

A Stable Lipid/TiO2 Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO2

Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

Spatial organization and signal transduction at intercellular junctions

Contact Info

Product

Resources

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A Stable Lipid/TiO₂ Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO₂

A Stable Lipid/TiO₂ Interface with Headgroup-Inversed Phosphocholine and a Comparison with SiO₂