Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

Hohner, Andreas; David, Maria Pamela C.; Rädler, Joachim O.

doi:10.1116/1.3319326

Cited by 81 publications

(117 citation statements)

References 44 publications

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“…The Pc@pNIPAM/lipid composite was fabricated via a facile solvent-exchange method [29]. An amount of 0.2 mg lipid (DOPC labeled by 1 mol% NBD-PE, green fluorescence) was first dissolved in chloroform (2.0 mg mL −1 ) and dried overnight under vacuum.…”

Section: Methodsmentioning

confidence: 99%

Encapsulation of Hydrophobic Phthalocyanine with Poly(N-isopropylacrylamide)/Lipid Composite Microspheres for Thermo-Responsive Release and Photodynamic Therapy

Liu

Zhang

et al. 2014

Materials

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Phthalocyanine (Pc) is a type of promising sensitizer molecules for photodynamic therapy (PDT), but its hydrophobicity substantially prevents its applications. In this study, we efficiently encapsulate Pc into poly(N-isopropylacrylamide) (pNIPAM) microgel particles, without or with lipid decoration (i.e., Pc@pNIPAM or Pc@pNIPAM/lipid), to improve its water solubility and prevent aggregation in aqueous medium. The incorporation of lipid molecules significantly enhances the Pc loading efficiency of pNIPAM. These Pc@pNIPAM and Pc@pNIPAM/lipid composite microspheres show thermo-triggered release of Pc and/or lipid due to the phase transition of pNIPAM. Furthermore, in the in vitro experiments, these composite particles work as drug carriers for the hydrophobic Pc to be internalized into HeLa cells. After internalization, the particles show efficient fluorescent imaging and PDT effect. Our work demonstrates promising candidates in promoting the use of hydrophobic drugs including photosensitizers in tumor therapies.

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Section: Methodsmentioning

confidence: 99%

Encapsulation of Hydrophobic Phthalocyanine with Poly(N-isopropylacrylamide)/Lipid Composite Microspheres for Thermo-Responsive Release and Photodynamic Therapy

Liu

Zhang

et al. 2014

Materials

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“…There are many techniques to create SLBs, including Langmuir-Blodgett/Schäfer deposition,[1] spin coating,[2] microcontact printing,[3] solvent-exchange deposition,[4] lipid-surfactant micelles,[5] evaporation induced assembly,[6] bubble collapse deposition,[7*] lipid dip-pen nanolithography,[8] and vesicle fusion. [9*] Langmuir-Blodgett/Schäfer (LB/LS) deposition and vesicle fusion are perhaps the most commonly used techniques to form SLBs.…”

Section: Introductionmentioning

confidence: 99%

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

Hardy

Nayak

Zauscher

2013

Current Opinion in Colloid & Interface Science

185

179

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Vesicle fusion has long provided an easy and reliable method to form supported lipid bilayers (SLBs) from simple, zwitterionic vesicles on siliceous substrates. However, for complex compositions, such as vesicles with high cholesterol content and multiple lipid types, the energy barrier for the vesicle-to-bilayer transition is increased or the required vesicle-vesicle and vesicle-substrate interactions are insufficient for vesicle fusion. Thus, for vesicle compositions that more accurately mimic native membranes, vesicle fusion often fails to form SLBs. In this paper, we review three approaches to overcome these barriers to form complex, biomimetic SLBs via vesicle fusion: (i) optimization of experimental conditions (e.g., temperature, buffer ionic strength, osmotic stress, cation valency, and buffer pH), (ii) α-helical (AH) peptide-induced vesicle fusion, and (iii) bilayer edge-induced vesicle fusion. AH peptide-induced vesicle fusion can form complex SLBs on multiple substrate types without the use of additional equipment. Bilayer edge-induced vesicle fusion uses microfluidics to form SLBs from vesicles with complex composition, including vesicles derived from native cell membranes. Collectively, this review introduces vesicle fusion techniques that can be generalized for many biomimetic vesicle compositions and many substrate types, and thus will aid efforts to reliably create complex SLB platforms on a range of substrates.

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“…The principle of the coating procedure is a controlled solvent-exchange deposition of the lipid onto the MOF surface. 20 For this purpose the lipid and the MOF nanoparticles are dispersed in an EtOH/H 2 O mixture, where the lipids exist as monomers. 21 When the water concentration is drastically increased, the lipids precipitate on the nanoparticle surface and form a lipid bilayer (Fig.…”

mentioning

confidence: 99%

MOF nanoparticles coated by lipid bilayers and their uptake by cancer cells

Wuttke¹,

Braig²,

et al. 2015

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We report the synthesis of MOF@lipid nanoparticles as a versatile and powerful novel class of nanocarriers based on metal-organic frameworks (MOFs). We show that the MOF@lipid system can effectively store dye molecules inside the porous scaffold of the MOF while the lipid bilayer prevents their premature release. Efficient uptake of the MOF@lipid nanoparticles by cancer cells makes these nanocarriers promising for drug delivery and diagnostic purposes.

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Controlled solvent-exchange deposition of phospholipid membranes onto solid surfaces

Cited by 81 publications

References 44 publications

Encapsulation of Hydrophobic Phthalocyanine with Poly(N-isopropylacrylamide)/Lipid Composite Microspheres for Thermo-Responsive Release and Photodynamic Therapy

Encapsulation of Hydrophobic Phthalocyanine with Poly(N-isopropylacrylamide)/Lipid Composite Microspheres for Thermo-Responsive Release and Photodynamic Therapy

Model cell membranes: Techniques to form complex biomimetic supported lipid bilayers via vesicle fusion

MOF nanoparticles coated by lipid bilayers and their uptake by cancer cells

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