Supported Bilayers On Silica

Interfacing Zwitterionic Liposomes with Inorganic Nanomaterials: Surface Forces, Membrane Integrity, and Applications

“…While most experiments were performed on planar silica surfaces, silica NPs were also studied. [17][18][19][20][21][22] These studies set a solid basis for exploring the interaction between PC liposomes with other types of inorganic NPs.…”

Lipid-based Biomimetics in Drug and Vaccine Delivery

“…Tris-hydroxymethylaminomethane (Tris) used as buffer increased affinity between PC and silica at pH  7.4 due to Tris adsorption on silica with an increase in the surface density of hydroxyls on the surface available to hydrogen bridging with phosphate phospholipid groups. Bilayer deposition, however, was unambiguosly confirmed by the three techniques only for the interaction DPPC vesicles/silica over 1 h at 65 o C and for the interaction DODAB vesicles/silica over the all range of experimental conditions tested (Rapuano & Carmona-Ribeiro, 2000). A simple spectrophotometric method for identifying entire bilayer deposition onto solid particles was developed from incorporation of the optical probe merocyanine 540 onto the outer bilayer vesicle surface.…”

“…Formation of ion pairs between the quaternary ammonium in the choline moiety of the phospholipid and the deprotonated silanol drove vesicle adhesion to the particle but vesicle rupture and bilayer deposition was determined by the cooperative occurrence of several hydrogen bridges between silanol and the phosphate moiety on the phospholipid (Rapuano & Carmona-Ribeiro, 1997). A low affinity between neutral phospholipids and the silica surface and a high affinity for the cationic amphiphile over a range of pH values was obtained (Rapuano & Carmona-Ribeiro, 2000). Tris-hydroxymethylaminomethane (Tris) used as buffer increased affinity between PC and silica at pH  7.4 due to Tris adsorption on silica with an increase in the surface density of hydroxyls on the surface available to hydrogen bridging with phosphate phospholipid groups.…”

“…From the comparison between langmuirian adsorption isotherms for PC, DPPC or DODAB deposition on silica particles (50 nm mean diameter), affinity constants for the surface increased in the following order PC<DPPC<DODAB (Rapuano and Carmona-Ribeiro, 1997;Rapuano and Carmona-Ribeiro, 2000). The molecular shape for PC in the bilayer is more cylindrical for PC than for DPPC, the geometric parameter for PC is larger than the geometric parameter for DPPC and it becomes difficult for the PC bilayer to bend and deposit on a curved silica particle but bending is easier for a DPPC bilayer.…”

Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

“…Synthesis of liposome-silica derivatives is a widely used method to stabilize the lipid bilayer [31][32][33][34].…”