Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay

Abstract

In: TALANTA, 134, 120-125, 2015. To refer to or to cite this work, please use the citation to the published version:Beloglazova N., Goryacheva O., Speranskaya E., Aubert T., Shmelin P. , Kurbangaleev V., Goryacheva I.Y., De Saeger S. (2015). Silica-coated liposomes loaded with quantum dots as labels for multiplex fluorescent immunoassay. TALANTA, 134,[120][121][122][123][124][125] AbstractThis manuscript describes synthesis and followed application of silica-coated liposomes loaded with quantum dots as a perspective label for immunoaasay. The hollow spherical structure of liposomes makes them an attractive package material for encapsulation of multiple water-insoluble quantum dots and amplifying the analytical signal. Silica coverage ensures the stability of the loaded liposomes against fusion and internal leakage during storage, transporting, application and also provides groups for bioconugation. For the first time these nanostructures were employed for the sensitive multiplex immunochemical determination of two analytes. As a model system mycotoxins zearalenone and aflatoxin B1 were detected in cereals. For simplification of multiassay results' evaluation the silanized liposomed loaded with QDs of different colors were used. The IC 50 values for the simultaneous determination of zearalenone and aflatoxin B1 were 16.2 and 18 µg kg -1 for zearalenone and 2.2 and 2.6 µg kg -1 for aflatoxin B1 in wheat and maize, respectively. As confirmatory method, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used.

Developments in mycotoxin analysis: an update for 2017-2018

“…The corresponding linear range in cereals was around 0.08-25 µg/kg. Beloglazova et al (2015) tested silicacoated liposomes loaded with QDs as labels for fluorescent immunoassays and developed an assay for concurrent ZEA and AFB 1 determination in cereals. The hollow spherical structure of liposomes allows encapsulation of multiple water-insoluble QDs, amplifying their signal.…”

High Throughput Detection Methods for Multiplex Mycotoxins

“…Chen et al applied antigen-modified magnetic nanoparticles as biosensor probes and antibody-functionalized improved upconversion nanoparticles as signal probes to develop an ultrasensitive fluorescence biosensor, which showed the LOD of 0.001 ng/mL and detection linear range of 0.001-0.1 ng/mL for AFB 1 and DON in peanut oils [102]. Saeger group used quantum dot (QD) nanolabels to develop the multiplex fluorescent immunosorbent simultaneous analysis method which has LODs of 3.2, 0.6, 0.2, 10 and 0.4 μg/kg for DON, ZEN, AFB 1 , T-2 and FB1 in maize [103]. Then they used QD @ SiO 2 and silica-coated liposomes loaded with QDs as labels to establish the multiplex fluorescent immunosorbent assay with the LODs of 6.1 and 5.3, 5.4, and 4.1, 2.6, and 1.9 μg/kg for DON, ZEN and AFB 1 [104], 16.2 and 18, 2.2 and 2.6 μg/kg for ZEN and AFB 1 in maize and wheat.…”

Determination of total aflatoxin using cysteamine-capped CdS quantum dots as a fluorescence probe

“…During recent years, several optical approaches based on GDs have been described for determination of different types of aflatoxins [40][41][42][43], specially aflatoxin B1 [44][45][46] ( Table 2). As these methods are based on monoclonal antibody of special type of aflatoxin and all types of aflatoxins can cause diseases [1], they cannot be used as a flexible method for quantification of total aflatoxin in solutions.…”