SUPPLIVER: Bioartificial supply for liver failure

Figaro, S.; Pereira, Ulysse; Dumé, A-S.; Rada, H.; Capone, S.; Bengrine, Abderrahmane; Baze, Audrey; Rabenirina, E.; Semenzato, N.; Herpe, Y-E.; Faivre, J; Dufresne, M.; Richert, Lysiane; Duverlie, Gilles; Daujat-Chavanieu, Martine; Saliba, Faouzi; Pouchoulin, Dominique; Legallais, Cécile

doi:10.1016/j.irbm.2015.01.010

Cited by 14 publications

(25 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While this additional layer of alginate on the PLL coating appeared essential for implantation of the encapsulated cells, this is not the case for extracorporeal circulation devices such as BAL therapy. In fact, most BAL devices, including the one developed in our laboratory (Figaro et al, 2015), include a plasma filter to separate the plasma from the elements formed of the blood. As a result, the cells of the immune system are discarded upstream, before making contact with the therapeutic biomass, making activation of a specific immune response unlikely.…”

Section: Discussionmentioning

confidence: 99%

Preclinical characterization of alginate‐poly‐L‐lysine encapsulated HepaRG for extracorporeal liver supply

Pasqua

Pereira

Lartigue

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

We recently demonstrated that HepaRG cells encapsulated into 1.5% alginate beads are capable of self-assembling into spheroids. They adequately differentiate into hepatocyte-like cells, with hepatic features observed at Day 14 post-encapsulation required for external bioartificial liver applications. Preliminary investigations performed within a bioreactor under shear stress conditions and using a culture medium mimicking acute liver failure (ALF) highlighted the need to reinforce beads with a polymer coating. We demonstrated in a first step that a poly-L-lysine coating improved the mechanical stability, without altering the metabolic activities necessary for bioartificial liver applications (such as ammonia and lactate elimination). In a second step, we tested the optimized biomass in a newly designed perfused dynamic bioreactor, in the presence of the medium model for pathological plasma for 6 h. Performances of the biomass were enhanced as compared to the steady configuration, demonstrating its efficacy in decreasing the typical toxins of ALF. This type of bioreactor is easy to scale up as it relies on the number of micro-encapsulated cells, and could provide an adequate hepatic biomass for liver supply. Its design allows it to be integrated into a hybrid artificial/bioartificial liver setup for further clinical studies regarding its impact on ALF animal models.

show abstract

Section: Discussionmentioning

confidence: 99%

Preclinical characterization of alginate‐poly‐L‐lysine encapsulated HepaRG for extracorporeal liver supply

Pasqua

Pereira

Lartigue

et al. 2020

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…While liver tissue does show regenerative properties, severe and acute liver failure is often fatal before natural regeneration can occur . The main constraint of liver tissue engineering is to obtain a large amount of hepatocytes, in vitro, able to fulfill the various liver functions over a long period of time .…”

Section: Tissue‐specific Coculture Processesmentioning

confidence: 99%

Multilineage Constructs for Scaffold‐Based Tissue Engineering: A Review of Tissue‐Specific Challenges

Baudequin

Tabrizian

2017

Adv Healthcare Materials

View full text Add to dashboard Cite

There is a growing interest in the regeneration of tissue in interfacial regions, where biological, physical, and chemical attributes vary across tissue type. The simultaneous use of distinct cell lineages can help in developing in vitro structures, analogous to native composite tissues. This literature review gathers the recent reports that have investigated multiple cell types of various sources and lineages in a coculture system for tissue-engineered constructs. Such studies aim at mimicking the native organization of tissues and their interfaces, and/or to improve the development of complex tissue substitutes. This paper thus distinguishes itself from those focusing on technical aspects of coculturing for a single specific tissue. The first part of this review is dedicated to variables of cocultured tissue engineering such as scaffold, cells, and in vitro culture environment. Next, tissue-specific coculture methods and approaches are covered for the most studied tissues. Finally, cross-analysis is performed to highlight emerging trends in coculture principles and to discuss how tissue-specific challenges can inspire new approaches for regeneration of different interfaces to improve the outcomes of various tissue engineering strategies.

show abstract

“…As already stated, the cell environment must ensure both efficient cell survival and optimization of biological functions. Several teams have thus proposed encapsulating hepatic cells in alginate-based beads (14)(15)(16)(17)(18). Alginate, thanks to its porous structure, is an appropriate cell niche making possible exchanges of nutrients, oxygen and metabolites, while sieving immunoglobulins from the patient's plasma.…”

Section: Introductionmentioning

confidence: 99%

“…Our group developed a new perfusion circuit named Suppliver, adapted from a plasmapheresis set on a Prismaflex® (Gambro/Baxter) device, placing 4 fluidized bed bioreactors on a plasma extraction line in a closed secondary loop (14). Changes were made to the classic plasmapheresis circuit in order to meet the specifications of BAL therapy (Fig.…”

Section: Introductionmentioning

confidence: 99%

Optimizing the Fluidized Bed Bioreactor as an External Bioartificial Liver

et al. 2017

View full text Add to dashboard Cite

Increasing bead density clearly maintained the performances of the fluidized bed with plasma of different compositions, without any risk of release out of the bioreactor. A 1% (v/v)-concentration of microspheres in alginate solution did not result in any alteration of the mechanical or biological behavior. This concentration can thus be applied to the production of large-scale encapsulated biomass for further use of the Suppliver setup in human scale preclinical studies.

show abstract

SUPPLIVER: Bioartificial supply for liver failure

Cited by 14 publications

References 9 publications

Preclinical characterization of alginate‐poly‐L‐lysine encapsulated HepaRG for extracorporeal liver supply

Preclinical characterization of alginate‐poly‐L‐lysine encapsulated HepaRG for extracorporeal liver supply

Multilineage Constructs for Scaffold‐Based Tissue Engineering: A Review of Tissue‐Specific Challenges

Optimizing the Fluidized Bed Bioreactor as an External Bioartificial Liver

Contact Info

Product

Resources

About