Supplementation with β-carotene or vitamin E protects against increase in anaphylactic response in β-lactoglobulin-sensitized Balb/c mice: ex vivo study

Grar, H.; Dib, W.; Mecherfi, Kamel Eddine El; Negaoui, H.; Gourine, H.; Benattia, S. Bekkaddour; Amier, L.; Kaddouri, H.; Khéroua, O.; Saïdi, D.

doi:10.1007/s00217-015-2471-5

Cited by 7 publications

(2 citation statements)

References 27 publications

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“… 2011 ; Grar et al. 2015 ). Usually, IgE-dependent food allergy reactions affect one or more target organs such as skin, respiratory tract, gastrointestinal tract and cardiovascular system (Sicherer 2002 ).…”

Section: Discussionmentioning

confidence: 99%

Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy

Guendouz

Haddi

Grar

et al. 2017

Pharmaceutical Biology

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Context: Royal jelly (RJ) has long been used to promote human health.Objective: The current study investigated the preventive effects of RJ against the development of a systemic and intestinal immune response in mice allergic to cow’s milk proteins.Materials and methods: Balb/c mice treated orally for seven days with RJ at doses of 0.5, 1 and 1.5 g/kg were sensitized intraperitoneally with β-lactoglobulin (β-Lg). Serum IgG and IgE anti-β-Lg were determined by an enzyme-linked immunosorbent assay (ELISA). Plasma histamine levels, symptom scores and body temperature were determined after in vivo challenge to β-Lg. Jejunums were used for assessment of local anaphylactic responses by an ex vivo study in Ussing chambers and morphologic changes by histological analysis.Results: RJ significantly decreased serum IgG (31.15–43.78%) and IgE (64.28–66.6%) anti-β-Lg and effectively reduced plasma histamine level (66.62–67.36%) (p < 0.001) at all the doses tested. Additionally, no clinical symptoms or body temperature drops were observed in RJ-pretreated mice. Interestingly, RJ significantly reduced (p < 0.001) intestinal dysfunction by abolishing the secretory response (70.73–72.23%) induced by sensitization and prevented length aberrations of jejunal villi by 44.32–59.01% (p < 0.001).Discussion and conclusions: We speculate that using RJ may help prevent systemic and anaphylactic response in allergic mice. These effects may be related to its inhibitory effects on the degranulation of mast cells.

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Section: Discussionmentioning

confidence: 99%

Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy

Guendouz

Haddi

Grar

et al. 2017

Pharmaceutical Biology

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“…The villi length in 2% bLf-immunized mice was significantly higher than that observed in control group ( Figure 5) (p < .016), although the magnitude of the increase was less than that in 5% and 10% bLf-immunized mice (p < .0001). Recently, Grar et al (2015) reported Figure 3. Clinical scores for hypersensitivity reactions responses in control and bLf mice after intraperitoneal challenge with bLf (n = 6).…”

Section: Histological Studymentioning

confidence: 99%

Bovine lactoferrin allergenicity as studied in murine model of allergy

Negaoui

Mecherfi

Tadjer

et al. 2016

Food and Agricultural Immunology

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In our study, we tried to investigate the allergenic properties of bovine lactoferrin (bLf) at different doses. The lactoferrin (LF) allergenicity was explored using murine model of allergy through measuring anti-LF IgG, IgG1 and IgE antibodies (Abs) responses and by in vivo anaphylactic reactions in LF sensitized mice. A histological examination of intestinal lamina propria was performed to assess gut inflammation. Four groups of mice (Balb/ c) were sensitized intraperitoneally to bLf using different doses (1%, 2%, 5% and 10%). Histological study of intestinal lamina propria revealed a marked decrease in villus length in 5% and 10% sensitized mice groups. Also, high levels of anti-bLf IgG and IgE were observed. Concerning the in vivo reactions, all groups developed clinical symptoms of anaphylactic reactions at different stages. Our findings show that LF-sensitized mice at 5% and 10% developed important clinical symptoms after intraperitoneal challenge with LF.

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Prophylactic properties of Bacillus subtilis in a bovine β-lactoglobulin sensitized mice model

Dib

Grar

Gourine

et al. 2019

Eur Food Res Technol

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Supplementation with β-carotene or vitamin E protects against increase in anaphylactic response in β-lactoglobulin-sensitized Balb/c mice: ex vivo study

Cited by 7 publications

References 27 publications

Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy

Preventive effects of royal jelly against anaphylactic response in a murine model of cow’s milk allergy

Bovine lactoferrin allergenicity as studied in murine model of allergy

Prophylactic properties of Bacillus subtilis in a bovine β-lactoglobulin sensitized mice model

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