Bovine lactoferrin allergenicity as studied in murine model of allergy

Negaoui, H.; Mecherfi, Kamel Eddine El; Tadjer, Sid Ahmed; Grar, H.; Khéroua, O.; Saïdi, D.

doi:10.1080/09540105.2016.1160365

Cited by 13 publications

(11 citation statements)

References 28 publications

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“…It has been shown previously that individuals with CMA have high levels of IgG (Høst et al, 1992). A high level of IgG was also observed in mice immunized with bovine lactoferrin (Negaoui et al, 2016). Our experiment showed that both casein fractions induced a high level of serumspecific IgG and IgA and similar levels of fecal IgA.…”

Section: Humoral Response Against Casein Fractions (In Vivo Study)supporting

confidence: 79%

Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies

Fuc

Złotkowska

Stachurska

et al. 2018

Journal of Dairy Science

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The aim of this study was to evaluate the ex vivo and in vivo studies immune potential of α-and κ-casein. Ex vivo, naïve mouse splenocytes were stimulated with α-or κ-casein. After 120 h of culture, the proliferation index (PI), determined by 3-(4,5 dimethylthiazol-2yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and carboxyfluorescein diacetate N-succinimidyl ester (CFSE) staining, did not vary for either antigen, suggesting similar ex vivo immunogenic potential of both casein fractions. In vivo, BALB/ccmdb mice were sensitized with α-or κ-casein and then gavaged with primary antigen. Mice immunized with α-casein had higher levels of IgG (2 16.33) and IgA (2 10.22) in serum at the end of the experiment compared with mice immunized with κ-casein (2 15 and 2 9.3 for IgG and IgA, respectively). The use of α-casein for mouse immunization and ex vivo lymphocyte stimulation resulted in higher concentrations of secreted cytokines (IL-4, IL-10) compared with κ-casein stimulation. This is consistent with increasing regulatory T cell (Treg) lymphocyte populations, independent of the antigen used for stimulation. In summary, the immunogenic potential of α-and κ-casein was similar. Humoral and cellular immune responses confirmed their strong, independent potential to induce B and T cells. We propose that the lymphocyte proliferation index be used as an initial screening for protein immunogenicity.

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Section: Humoral Response Against Casein Fractions (In Vivo Study)supporting

confidence: 79%

Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies

Fuc

Złotkowska

Stachurska

et al. 2018

Journal of Dairy Science

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“…Concerning the in vivo reactions, all groups developed clinical symptoms of anaphylactic reactions at different stages. Their findings show that LF-sensitised mice at 5% and 10% developed important clinical symptoms after intraperitoneal challenge with LF (Negaoui et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Sensitisation to molecular components in patients with atopic dermatitis, relation to asthma bronchiale and allergic rhinitis

Čelakovská

Bukač

Vaňková

et al. 2020

Food and Agricultural Immunology

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Backround and aim: To evaluate the sensitisation to molecular components in atopic dermatitis patients with the use of Multiplex ISAC testing. Method: The complete dermatological and allergological examination including the examination of the sensitisation to molecular components with Multiplex ISAC testing was performed. Results and conclusion: Eighty one patients were examined -40 men, 41 women, average age 41.7 years. The positivity was recorded most frequently to molecular components of timothy, birch, house dust mite, animals dander, bermuda grass, peach, apple, hazel pollen, kiwi, peanut and hazelnut, Alternaria and Aspergillus. Molecular components such as Equ c 1 (Horse), Alt a 6 (Alternaria), Fel d 1, Fel d 4 (Cat) and Can f 1, Can f 5 (Dog) are recorded with the significant higher occurrence simultaneously in patients suffering from severe form of AD, asthma bronchiale and allergic rhinitis; these molecular components play the most important role in the atopic march.

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“…Together with lactoferrin, bovine serum albumin is characterised by a high number of disulphide bridges ( Table 1 ), making their tertiary structure highly stable even under denaturing conditions [ 64 ]. Lactoferrin is a protein not registered as an allergen in the AllergenNomenclature [ 56 ], however, human and animal experimental studies showed their ability to induce allergic reactions [ 65 , 66 , 67 ].…”

Section: Food Allergymentioning

confidence: 99%

Alternatives to Cow’s Milk-Based Infant Formulas in the Prevention and Management of Cow’s Milk Allergy

Maryniak

Sancho

Hansen

et al. 2022

Foods

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Cow’s milk-based infant formulas are the most common substitute to mother’s milk in infancy when breastfeeding is impossible or insufficient, as cow’s milk is a globally available source of mammalian proteins with high nutritional value. However, cow’s milk allergy (CMA) is the most prevalent type of food allergy among infants, affecting up to 3.8% of small children. Hypoallergenic infant formulas based on hydrolysed cow’s milk proteins are commercially available for the management of CMA. Yet, there is a growing demand for more options for infant feeding, both in general but especially for the prevention and management of CMA. Milk from other mammalian sources than the cow, such as goat, sheep, camel, donkey, and horse, has received some attention in the last decade due to the different protein composition profile and protein amino acid sequences, resulting in a potentially low cross-reactivity with cow’s milk proteins. Recently, proteins from plant sources, such as potato, lentil, chickpeas, quinoa, in addition to soy and rice, have gained increased interest due to their climate friendly and vegan status as well as potential lower allergenicity. In this review, we provide an overview of current and potential future infant formulas and their relevance in CMA prevention and management.

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Bovine lactoferrin allergenicity as studied in murine model of allergy

Cited by 13 publications

References 28 publications

Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies

Immunoreactive properties of α-casein and κ-casein: Ex vivo and in vivo studies

Sensitisation to molecular components in patients with atopic dermatitis, relation to asthma bronchiale and allergic rhinitis

Alternatives to Cow’s Milk-Based Infant Formulas in the Prevention and Management of Cow’s Milk Allergy

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