Supplementation with Niacin during in vitro maturation improves the quality of porcine embryos

Almubarak, Areeg; Kim, Eunji; Yu, In Ho; Jeon, Yubyeol

doi:10.1016/j.theriogenology.2021.04.005

Cited by 19 publications

(24 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Consistent with this, previous studies have also shown an improvement in oocyte quality when mouse follicles cultured in vitro were supplemented with NA [ 29 ]. Bovine oocytes treated with 400 µM NA also exhibited significant improvements in meiotic progression and developmental competence following vitrification [ 30 ], and blastocyst formation was significantly improved when porcine oocytes isolated from large antral follicles were supplemented with 600 µM niacin during IVM [ 31 ]. There was no effect on aged, in vitro matured murine oocytes at low doses of NA [ 18 ], however, a significant increase in intrinsic oocyte NAD + levels in the oocytes from aged mice compared with control mice was observed [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

Supplementing media with NAD+ precursors enhances the in vitro maturation of porcine oocytes

et al. 2021

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Supplementing media with NAD+ precursors enhances the in vitro maturation of porcine oocytes

et al. 2021

View full text Add to dashboard Cite

“…The observed rescue in spindle length and width with the inclusion of NA may be attributed to the protective effects of NA under stress conditions through an increase in intracellular NAD + levels [ 49 , 50 ]. Previously, supplementation during IVM with NA was found to improve the developmental competence of bovine [ 51 ] and porcine oocytes [ 44 , 52 ]. Confirmation that NAPRT is expressed in mouse oocytes [ 24 ] provides further evidence that the Preiss-Handler pathway is important for NAD + biosynthesis during oocyte maturation.…”

Section: Discussionmentioning

confidence: 99%

Insights into the NAD+ biosynthesis pathways involved during meiotic maturation and spindle formation in porcine oocytes

et al. 2022

View full text Add to dashboard Cite

Treatments that elevate NAD + levels have been found to improve oocyte quality in mice, cattle, and pigs, suggesting that NAD + is vital during oocyte maturation. This study aimed to examine the influence of different NAD + biosynthetic pathways on oocyte quality by inhibiting key enzymes. Porcine oocytes from small antral follicles were matured for 44 h in a defined maturation system supplemented with 2-hydroxynicotinic acid [2-HNA, nicotinic acid phosphoribosyltransferase (NAPRT) inhibitor], FK866 [nicotinamide phosphoribosyltransferase (NAMPT) inhibitor], or gallotannin [nicotinamide mononucleotide adenylyltransferase (NMNAT) inhibitor] and their respective NAD + pathway modulators (nicotinic acid, nicotinamide, and nicotinamide mononucleotide, respectively). Cumulus expansion was assessed after 22 h of maturation. At 44 h, maturation rates were determined and mature oocytes were fixed and stained to assess spindle formation. Each enzyme inhibitor reduced oocyte maturation rate and adversely affected spindle formation, indicating that NAD + is required for meiotic spindle assembly. Furthermore, NAMPT and NMNAT inhibition reduced cumulus expansion, whereas NAPRT inhibition affected chromosomal segregation. Treating oocytes with gallotannin and nicotinamide mononucleotide together showed improvements in spindle width, while treating oocytes with 2-HNA and nicotinic acid combined showed an improvement in both spindle length and width. These results indicate that the salvage pathway plays a vital role in promoting oocyte meiotic progression, while the Preiss-Handler pathway is essential for spindle assembly.

show abstract

“…Şimdiye kadar yapılan çalışmalar, artan ROS ‹un üreme potansiyelini olumsuz yönde etkilediğini ve granüloza hücre ölümünü indüklediğini göstermektedir (30)(31). Granüloza hücre ölümü foliküler oositleri besleyici maddelerden, büyüme ve sağ kalım faktörlerinden ayırır ve östradiol biyosentezini azaltır (32)(33).…”

Section: Discussionunclassified

“…Bütün bunlara bağlı olarak ovaryen bozukluklar ve infertilite ortaya çıkar. Antioksidanların, diğer moleküllerin oksidasyonunu yavaşlatabildiği veya önleyebildiği ve hücresel hasara neden olabilecek serbest radikallerin üretimini azaltma potansiyeli taşıdığı düşünülmektedir (30)(31)(32)(33)(34).…”

Section: Discussionunclassified

İnferti̇l Hastalarin Granüloza Hücreleri̇nde Li̇qui̇ri̇ti̇geni̇n’i̇n Lösemi̇ İnhi̇bi̇tör Faktör Reseptörü Üzeri̇ne Etki̇leri̇

Usta¹,

Köktürk²,

Doğan³

et al. 2022

jarhs

View full text Add to dashboard Cite

Objectives: Granulosa cells have an important role in oocyte nutrition and maturation, oocyte-sperm interaction events such as capacitation, and acrosome reaction. Therefore, studies with granulosa cells are also important for the healthy development of the oocyte and fertilization. The aim of this study was to investigate the effects of liquiritigenin (LQ) in vitro on granulosa cells that were obtained from the oocyte-cumulus complex of patients who were treated in the in vitro fertilization (IVF) unit due to ovulatory factor (OF, infertile) and male factor (MF, healthy). Materials and Methods:This study included patients who were treated in the IVF unit of the Istanbul University Faculty of Medicine due to OF and MF. Granulosa cells obtained from 5 MF and 5 OF patients were divided into MF, MF-Dimethyl sulfoxide (DMSO), MF-LQ, OF, OF-DMSO and OF-LQ groups in culture medium. After the application, the cells were stained with the leukemia inhibitory factor receptor (LIFR) immunocytochemical staining and the staining intensity was examined with the Aperio ImageScope analysis software and cell morphology. Results:The LIFR immunocytochemistry staining intensity in the granulosa cells of the OF and OF-DMSO groups was significantly decreased when compared to the MF and MF-DMSO groups. The LIFR staining intensity in the granulosa cells of the MF-LQ and OF-LQ groups were determined to be significantly higher than the other groups. In the granulosa cells of OF and OF-DMSO groups, the findings reflected apoptotic cell morphology, but showed no or less apoptotic cell morphology in the MF-LQ and OF-LQ groups. Conclusions: In granulosa cells of LQ-groups, LIFR immunocytochemistry staining intensity algorithms were obtained as significantly higher than in the especially the OF and OF-DMSO groups, and showed the positive effects on apoptotic cell morphology. These results suggest that LQ may be used as an alternative medium agent in IVF units. Therefore, more comprehensive studies are needed for the clinical use of LQ.

show abstract

Supplementation with Niacin during in vitro maturation improves the quality of porcine embryos

Cited by 19 publications

References 52 publications

Supplementing media with NAD<sup>+</sup> precursors enhances the <i>in vitro</i> maturation of porcine oocytes

Supplementing media with NAD<sup>+</sup> precursors enhances the <i>in vitro</i> maturation of porcine oocytes

Insights into the NAD<sup>+</sup> biosynthesis pathways involved during meiotic maturation and spindle formation in porcine oocytes

İnferti̇l Hastalarin Granüloza Hücreleri̇nde Li̇qui̇ri̇ti̇geni̇n’i̇n Lösemi̇ İnhi̇bi̇tör Faktör Reseptörü Üzeri̇ne Etki̇leri̇

Contact Info

Product

Resources

About