Supplementation with a complex of active nutrients improved dermal and epidermal characteristics in skin equivalents generated from fibroblasts from young or aged donors

Abstract

Cultured skin equivalent (SE, Mimeskin) was generated by co-culturing skin fibroblasts and keratinocytes on a collagen-glycosaminoglycan-chitosan dermal substrate. In order to examine donor age effect, fibroblasts from 19- (young) or 49- (aged) year-old females were used. Culture medium was supplemented with nutrients complex containing soy extract, tomato extract, grape seed extract, white tea extract, sodium ascorbate, tocopherol acetate, zinc gluconate and BioMarine complex. Epidermal and dermal structure and composition were examined after 42 and 60 days of culture. In untreated samples, SE generated from young fibroblasts was superior to SE from aged fibroblasts in all characteristics. Those include number and regularity of keratinocyte layers, number of keratinocytes expressing proliferation marker Ki67, content of collagen type I, fibrillin-1, elastin, and SE lifespan. Effects of nutritional supplementation were observed in SE from both young and aged fibroblasts, however, those effects were more pronounced in SE from aged fibroblasts. In epidermis, the treatment increased number of keratinocyte layers and delayed epidermal senescence. The number of cells expressing Ki67 was nine folds higher than those of controls, and was similar to that of young cell SE. In dermis, the treatment increased mRNA synthesis of collagen I, fibrillin-1 and elastin. In conclusion, skin cell donor age had major important effect on formation of reconstructed SE. Imperfections in epidermal and dermal structure and composition as well as life span in SE from aged cells can be improved by supplementation with active nutrients.

3D Cell Printing of Perfusable Vascularized Human Skin Equivalent Composed of Epidermis, Dermis, and Hypodermis for Better Structural Recapitulation of Native Skin

“…Another cause of this significant difference on the Ki67 might be due to the excessive expression of Ki67 in the suprabasal layers of the native epidermis. Despite the fact that the Ki67 is mostly expressed in the basal layer of epidermis, a large number of Ki67‐stained suprabasal layers were observed in this study. We suspect that the frozen skin samples embedded in OCT compound were not sectioned in a vertical plane.…”

Model of in vitro Healing to Test the Influence of Dedifferentiated <i>Crithmum maritimum</i> Cells on Dermal Repair and Epidermal Regeneration

“…In addition, the free amino acids that constitute the 'natural moisturizing factor' involved in keeping the skin hydrated have their origin in the cleavage of profilaggrin into filaggrin [19,20] . However, our model does not allow studying skin permeability as described by Henning et al [21] , Mitriaikina et al [22] and Nicoli et al [23] , but testing activity and the tolerance of actives [24,25] .…”

Adipose-derived Stem Cells Promote Skin Homeostasis and Prevent its Senescence in an In vitro Skin Model

“…Effectively, as multipotent stem cells, ASCs have the capacity to differentiate into fibroblasts [49]. Hence, in SE with 25% ASCs, the fibroblast-rich dermis provides a better quality DE, which in turn affects the quality of the epidermis, as previously shown [84,85]. Moreover, transdifferentiation of ASC into epithelial cells [50,[85][86][87][88][89][90] and migration toward the epidermis cannot be excluded.…”