Supplementation Vitrified-thawed Media with Melatonin Do Not Protecting Immature Mouse Testicular Tissue from Vitrified-thawed Induced Injury

This study aimed to assess the morphological changes in neonate vitrified testicular grafts after host treatment with melatonin. Neonate vitrified testes, candidates for transplantation to treated and nontreated groups receiving melatonin, were thawed in media containing or not containing 100 μm melatonin. Following transplantation, melatonin (20 mg kg(-1) per day) and saline were given to the treated and nontreated groups for up to 9 weeks. The testicular status was assayed by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick-end labelling TUNEL, semi-thin section and ultra-structure studies. Chi-squared test was used to compare categorical variables between the groups. Overall, the degenerative and apoptosis changes in the vitrified testis parenchyma were not significant. However, atrophic seminiferous cords and jumbled appearance of the interstitial space were more often observed in the nontreated group than in the treated ones. Semi-thin sections showed that the germinal epithelium was taken in a normal arrangement on the testicular grafts of both groups. Nevertheless, ultrastructural analysis revealed that the characteristics of interstitial space cells, basement membrane BM and epithelial cells of seminiferous tubules in the treated group were better than those in the nontreated group. The study revealed a beneficial effect of melatonin on vitrified neonatal testes and after that, on restoring testicular grafts.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Supplementation Vitrified-thawed Media with Melatonin Do Not Protecting Immature Mouse Testicular Tissue from Vitrified-thawed Induced Injury

Cited by 7 publications

References 41 publications

Does prepubertal testicular tissue vitrification influence spermatogonial stem cells (SSCs) viability?

Does prepubertal testicular tissue vitrification influence spermatogonial stem cells (SSCs) viability?

Evaluation of alginate hydrogel cytotoxicity on three-dimensional culture of type A spermatogonial stem cells

Potential use of melatonin supplementation to protect vitrified testicular grafts from hypoxic-ischaemic damage

Contact Info

Product

Resources

About