Evaluation of alginate hydrogel cytotoxicity on three-dimensional culture of type A spermatogonial stem cells

Jalayeri, Maryam; Pirnia, Afshin; Najafabad, Elaheh Poorazizi; Varzi, Ali Mohammad; Gholami, Mohammadreza

doi:10.1016/j.ijbiomac.2016.10.074

Cited by 26 publications

(23 citation statements)

References 17 publications

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“…Due to presence of SSCs on the basement membrane in a 3D microenvironment, the extracellular matrix may play an important role in the behavior of these cells. Hence, various 3D culture systems such as soft agar culture system (SACS), methylcellulose culture system (MCS), collagen gel matrix and alginate capsule have been developed for in vitro proliferation and differentiation of SSCs (Lee et al, 2006;Huleihel et al, 2015;Jalayeri et al, 2017;Navid et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

et al. 2019

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Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a promising technique for the proliferation and differentiation of SSCs in vitro. The goal of the present study was to investigate the effects of PCL/Gel nanofibrous scaffold on the propagation and differentiation of neonate mouse SSCs (mSSCs). mSSCs were enzymatically isolated, and the cells were purified by differential plating method and seeded on scaffold. After 2 weeks, viability, colony number and diameter, and expression of specific spermatogonial cell genes were investigated. After mSSCs propagation, the cells were cultivated in a differentiation medium on the scaffold for another 2 weeks, and differentiating cells were analyzed by real-time PCR. The number of mSSC colony (P<0.01) and expression levels of specific spermatogonial genes Plzf and Inga6 (P<0.01) and also differentiation genes c-Kit, Tp1 and Ptm1 (P<0.05) were higher in scaffold group compared with control during the culture period. We conclude that mSSCs can be expanded and can differentiate toward spermatid cells on PCL/Gel nanofibrous scaffold with improved developmental parameters.

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Section: Introductionmentioning

confidence: 99%

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

et al. 2019

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“…Encapsulation and local drug delivery systems have been the object of several reports concerning the testicle [ 129 , 141 , 153 , 154 , 155 ].…”

Section: Resultsmentioning

confidence: 99%

“…Alginate hydrogel displayed low cytotoxicity in 3D culture of mice prepubertal male germ cells [ 155 ]. Moreover, when used for encapsulation of bull germ cells during in vitro culture, it allowed differentiation up to the stage of haploid cells [ 129 ].…”

Section: Resultsmentioning

confidence: 99%

Tissue Engineering to Improve Immature Testicular Tissue and Cell Transplantation Outcomes: One Step Closer to Fertility Restoration for Prepubertal Boys Exposed to Gonadotoxic Treatments

Vento

Vermeulen

Michele

et al. 2018

IJMS

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Despite their important contribution to the cure of both oncological and benign diseases, gonadotoxic therapies present the risk of a severe impairment of fertility. Sperm cryopreservation is not an option to preserve prepubertal boys’ reproductive potential, as their seminiferous tubules only contain spermatogonial stem cells (as diploid precursors of spermatozoa). Cryobanking of human immature testicular tissue (ITT) prior to gonadotoxic therapies is an accepted practice. Evaluation of cryopreserved ITT using xenotransplantation in nude mice showed the survival of a limited proportion of spermatogonia and their ability to proliferate and initiate differentiation. However, complete spermatogenesis could not be achieved in the mouse model. Loss of germ cells after ITT grafting points to the need to optimize the transplantation technique. Tissue engineering, a new branch of science that aims at improving cellular environment using scaffolds and molecules administration, might be an approach for further progress. In this review, after summarizing the lessons learned from human prepubertal testicular germ cells or tissue xenotransplantation experiments, we will focus on the benefits that might be gathered using bioengineering techniques to enhance transplantation outcomes by optimizing early tissue graft revascularization, protecting cells from toxic insults linked to ischemic injury and exploring strategies to promote cellular differentiation.

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“…10 c-Kit 12 and synaptonemal complex protein 3 (SCP3) 13 are markers for differentiation of spermatogonia. 3 Somatic cells (Sertoli and Leydig) have cross-talks with SCs in their niche. 14 Approaches into the application of methods for tracing proliferative and survival traits in the germ cells along with enrichment of SCs are important for improving SC transplantation in infertile cases.…”

Section: Introductionmentioning

confidence: 99%

“…1 SCs are undifferentiated unipotent stem cells that placed near to the basement membrane of seminiferous tubules. 2 Due to occupying a very low number of cells (about 1%) with low mitotic divisions, 3 SCs are poorly distinguishable for morphological or biological features from other cells, and that knowledge about their stem cell property is too weak. 4 Lack of specific markers is another burden for detection of these cells.…”

Section: Introductionmentioning

confidence: 99%

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells

Toolee

Rastegar

Solhjoo

et al. 2018

J of Cellular Biochemistry

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Kisspeptin (Kp) expression in testis has caused most of the recent research surveying its functional role in this organ. This peptide influences spermatogenesis and sperm capacitation, so it is considered as a regulator of reproduction. Kp roles exert through hypothalamic/pituitary/gonadal axis. We aimed to evaluate direct roles for Kp on proliferation and differentiation of spermatogonial cells (SCs) when the cells are cocultured with somatic cells. Somatic cells and SCs were isolated from adult azoospermic and newborn mice and then enriched using a differential attachment technique. After the evaluation of identity and colonization for SCs, the cells were cocultured with somatic cells, and three doses of Kp (10−8‐10−6 M) was assessed on proliferation (through evaluation of MVH and ID4 markers) and differentiation (via evaluation of c‐Kit and SCP3, TP1, TP2, and, Prm1 markers) of the coculture system. Investigations were continued for four succeeding weeks. At the end of each level of testosterone in the culture media was also evaluated. We found positive influence from Kp on proliferative and differentiative markers in SCs cocultured with somatic cells. These effects were dose‐dependent. There was no effect for Kp on testosterone level. From our findings, we simply conclude that Kp as a neuropeptide for influencing central part of reproductive axis could also positively affect peripheral processes related to spermatogenesis without having an effect on steroidogenesis.

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Evaluation of alginate hydrogel cytotoxicity on three-dimensional culture of type A spermatogonial stem cells

Cited by 26 publications

References 17 publications

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

Proliferation and Differentiation of Mouse Spermatogonial Stem Cells on a Three-Dimensional Surface Composed of PCL/Gel Nanofibers

Tissue Engineering to Improve Immature Testicular Tissue and Cell Transplantation Outcomes: One Step Closer to Fertility Restoration for Prepubertal Boys Exposed to Gonadotoxic Treatments

Roles for Kisspeptin in proliferation and differentiation of spermatogonial cells isolated from mice offspring when the cells are cocultured with somatic cells

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