Supplementation of suckling rats with cow's milk induces hyperphagia and higher visceral adiposity in females at adulthood, but not in males

Rodrigues, Vanessa Silva Tavares; Moura, Egberto Gaspar de; Bernardino, Dayse Nascimento; Carvalho, Janaíne Cavalcanti; Soares, P.N.; Peixoto, Thamara Cherem; Peixoto-Silva, Nayara; Oliveira, Elaine de; Lisboa, Patrícia Cristina

doi:10.1016/j.jnutbio.2017.12.001

Cited by 11 publications

(7 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nuclear magnetic resonance (NMR) for small living animals was performed for the evaluation of total fat mass at PND148 using whole‐body composition analyzer NMR equipment (Bruker's Minispec LF90 TD‐NMR), as previously reported (Rodrigues et al, 2018 ). A quality control check of internal voltages, temperature, magnets, and NMR parameters was performed using a standard provided by the manufacturer.…”

Section: Methodsmentioning

confidence: 99%

The model of litter size reduction induces long‐term disruption of the gut‐brain axis: An explanation for the hyperphagia of Wistar rats of both sexes

Rodrigues

Moura

Peixoto

et al. 2022

Physiological Reports

Self Cite

View full text Add to dashboard Cite

The gut microbiota affects the host's metabolic phenotype, impacting health and disease. The gut‐brain axis unites the intestine with the centers of hunger and satiety, affecting the eating behavior. Deregulation of this axis can lead to obesity onset. Litter size reduction is a well‐studied model for infant obesity because it causes overnutrition and programs for obesity. We hypothesize that animals raised in small litters (SL) have altered circuitry between the intestine and brain, causing hyperphagia. We investigated vagus nerve activity, the expression of c‐Fos, brain‐derived neurotrophic factor (BDNF), gastrointestinal (GI) hormone receptors, and content of bacterial phyla and short‐chain fatty acids (SCFAs) in the feces of adult male and female Wistar rats overfed during lactation. On the 3rd day after birth, litter size was reduced to 3 pups/litter (SL males or SL females) until weaning. Controls had normal litter size (10 pups/litter: 5 males and 5 females). The rats were killed at 5 months of age. The male and female offspring were analyzed separately. The SL group of both sexes showed higher food consumption and body adiposity than the respective controls. SL animals presented dysbiosis (increased Firmicutes , decreased Bacteroidetes ) and had increased vagus nerve activity. Only the SL males had decreased hypothalamic GLP‐1 receptor expression, while only the SL females had lower acetate and propionate in the feces and higher CCK receptor expression in the hypothalamus. Thus, overfeeding during lactation differentially changes the gut‐brain axis, contributing to hyperphagia of the offspring of both sexes.

show abstract

Section: Methodsmentioning

confidence: 99%

The model of litter size reduction induces long‐term disruption of the gut‐brain axis: An explanation for the hyperphagia of Wistar rats of both sexes

Rodrigues

Moura

Peixoto

et al. 2022

Physiological Reports

Self Cite

View full text Add to dashboard Cite

show abstract

“…For this, rats were scanned using the Whole Body Composition Analyzer NMR equipment (Bruker's Minispec LF90 TD-NMR, Rheinstetten, Germany), as previously reported. 47 A quality control check of internal voltages, temperature, magnets, and NMR parameters was performed using a standard provided by the manufacturer. Non-anesthetized animals were placed in a clear plastic cylinder and restrained by insertion of a tight-fitting plunger into the cylinder.…”

Section: Total Fat Mass Evaluationmentioning

confidence: 99%

Neonatal nicotine exposure changes insulin status in fat depots: sex-related differences

Rodrigues

Miranda

Soares

et al. 2021

J Dev Orig Health Dis

Self Cite

View full text Add to dashboard Cite

Nicotine is the main psychoactive substance present in cigarette smoke that is transferred to the baby by breast milk. In rats, maternal nicotine exposure during breastfeeding induces obesogenesis and hormone dysfunctions in adult male offspring. As glucocorticoid (GC), insulin, and vitamin D change both adipogenesis and lipogenesis processes, we assessed parameters related to metabolism and action of these hormones in visceral and subcutaneous adipose tissues (VAT and SAT) of adult male and female rats in a model of neonatal nicotine exposure. At postnatal (PN) day 2, dams were kept with six pups (three per sex) and divided into nicotine and control groups for implantation of osmotic minipumps that released 6 mg/kg nicotine or saline, respectively. At PN180, fat mass, hormone levels, and protein contents of biomarkers of the GC activation and receptor (11beta-hydroxysteroid dehydrogenase type 1 and glucocorticoid receptor alpha), insulin signaling pathway [insulin receptor beta (IRβ), phosphorylated insulin receptor substrate 1, insulin receptor substrate 1 (IRS1), phosphorylated serine/threonine kinase (pAKT), serine/threonine kinase, glucose transporter type 4 (GLUT4)], and vitamin D activation and receptor (1α-hydroxylase and vitamin D receptor) were evaluated. While nicotine-exposed males showed increased fat mass, hypercorticosteronemia, hyperinsulinemia, and higher 25-hydroxyvitamin D, these alterations were not observed in nicotine-exposed females. Nicotine-exposed males only showed lower IRS1 in VAT, while the females had hyperglycemia, higher pAKT in VAT, while lower IRβ, IRS1, and GLUT4 in SAT. Parameters related to metabolism and action of GC and vitamin D were unaltered in both sexes. We evidence that exposure exclusively to nicotine during breastfeeding affects the hormone status and fat depots of the adult progeny in a sex-dependent manner.

show abstract

“…Same-day-old pups were immediately mixed and redistributed into small and large litters for maternal rearing. It should be noted that while some studies count the day of birth as postnatal day (PND) 1 (80) , others mark the day of parturition as PND 0 (81) . Though the use of PND 0 predominates (see Table 1), whatever protocol is adopted should be indicated.…”

Section: Altered Litter Size Modelmentioning

confidence: 99%

Rodent models of metabolic disorders: considerations for use in studies of neonatal programming

et al. 2021

View full text Add to dashboard Cite

Epidemiologically, metabolic disorders have garnered much attention, perhaps due to the predominance of obesity. The early postnatal life represents a critical period for programming multifactorial metabolic disorders of adult life. Though, altricial rodents are prime subjects for investigating neonatal programming, there is still no sufficiently generalised literature on their usage and methodology. This review focuses on establishing five approach-based models of neonatal rodents adopted for studying metabolic phenotypes. Here, some modelled interventions that currently exist to avoid or prevent metabolic disorders are also highlighted. We also bring forth recommendations, guidelines, and considerations to aid research on neonatal programming. It is hoped that this provides a background to researchers focused on the aetiology, mechanisms, prevention and treatment of metabolic disorders.

show abstract

Supplementation of suckling rats with cow's milk induces hyperphagia and higher visceral adiposity in females at adulthood, but not in males

Cited by 11 publications

References 69 publications

The model of litter size reduction induces long‐term disruption of the gut‐brain axis: An explanation for the hyperphagia of Wistar rats of both sexes

The model of litter size reduction induces long‐term disruption of the gut‐brain axis: An explanation for the hyperphagia of Wistar rats of both sexes

Neonatal nicotine exposure changes insulin status in fat depots: sex-related differences

Rodent models of metabolic disorders: considerations for use in studies of neonatal programming

Contact Info

Product

Resources

About