Supplementation of salvianic acid A to boar semen extender to improve seminal quality and antioxidant capacity

Tian, Xuekai; Liu, Dong; He, Yulin; Zhang, Wenyu; He, Hao; Rong-bin, DU; Pang, Wei; Yang, Gongshe; Yu, Taiyong

doi:10.1111/asj.13263

Cited by 11 publications

(12 citation statements)

References 26 publications

(32 reference statements)

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“…In addition, lipid peroxidation can also damage sperm. Experiments have proved that adding antioxidants to the semen extender of goats [ 37 ], cattle [ 38 ], pigs [ 39 ] and horses [ 40 ] can effectively improve sperm motility and eventually improve the effect of artificial insemination [ 41 ], which is of great significance for the development of animal husbandry.…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Wang

Zhang

Sohail

et al. 2022

Animals

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The purpose of this study was to investigate whether the addition of chlorogenic acid (CGA) to a sheep semen extender could improve the quality of chilled sheep sperm. Ejaculates (n = 80) were collected from five Hu rams with an artificial vagina. The ejaculates were mixed and divided into five equal parts, diluted with a CGA-free Tris–egg yolk extender (control), or supplemented with 0.2, 0.4, 0.8, and 1.2 mg/mL. The sperm kinematic parameters (viability, progressive motility), functional integrity of plasma membrane and acrosome, adenosine triphosphate (ATP) concentration and antioxidant parameters (Catalase (CAT), Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), ROS level and Malondialdehyde (MDA) content) were evaluated during storage of the semen. The results indicated that: PM, plasmatic membrane integrity and acrosomal integrity in 0.8 mg/mL CGA were higher (p < 0.05) from day 1 to 5. The ROS level in CGA groups was lower than the control (p < 0.05). CAT, SOD, ATP, and T-AOC were highest at 0.8 mg/mL concentration within 1 to 5 days. The above results indicated that the right concentration of CGA improved the quality of Hu ram sperm during chilling storage.

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Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Wang

Zhang

Sohail

et al. 2022

Animals

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“…Therefore, research on the effective composition of sperm preservation solution is very important for semen preservation. For example, adding some reagents or drugs, such as oligomeric proanthocyanidin [ 3 ], kojic acid [ 4 ], salvianolic acid A [ 5 ], to the sperm diluent will significantly affect sperm motility and in vitro preservation time.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature

Shen,

Fu,

Bai

et al. 2024

Anim Biosci

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Objective: The purpose of this study was to explore the effect of sodium salicylate (SS) on semen preservation and metabolic regulation in goats.Methods: Under the condition of low temperature, SS was added to goat semen diluent to detect goat sperm motility, plasma membrane, acrosome, antioxidant capacity, mitochondrial membrane potential (MMP) and metabonomics.Results: The results show that at the 8th day of low-temperature storage, the sperm motility of the 20 μM SS group was 66.64%, and the integrity rates of the plasma membrane and acrosome were both above 60%, significantly higher than those of the other groups. The activities of catalase and superoxide dismutase in the sperm of the 20 μM SS group were significantly higher than those of the control group, the contents of reactive oxygen species and malondialdehyde were significantly lower than those in the control group, the MMP was significantly higher than that in the control group, and the contents of Ca<sup>2+</sup> and total cholesterol were significantly higher than those in the control group. Through metabonomics analysis, there were significant metabolic differences between the control group and the 20 μM SS group. Twenty of the most significant metabolic markers were screened, mainly involving five metabolic pathways, of which nicotinic acid and nicotinamide metabolic pathways were the most significant.Conclusion: The results indicate that SS can effectively improve the low-temperature preservation quality of goat sperm.

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“…Hence, it is very important to study the pig sperm preserved at 17℃ in research and production. [14,29]. However, during the sperm preservation, sperm suffers from severe oxidative stress due to the accumulation of ROS which could decrease the sperm quality, compromise to the plasma membrane, the acrosome, the DNA integrity and the protein [12,24,28].…”

Section: Introductionmentioning

confidence: 99%

Hydroxytyrosol effectively improves the quality of pig sperm at 17 °C

Zhang

Tian

et al. 2022

Theriogenology

Self Cite

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Background: Arti cial insemination technology is the production technology used in more than 99% of the world's pig farms. As the frozen sperm damage is very serious, the preservation of pig sperm is mainly carried out at 17°C. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen species and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol group (optimum concentration). Ultimately, we selected the control group and the 120 μmol/L hydroxytyrosol group to test the effect of arti cial insemination.Results: The results of our research showed that during storage of pig sperm at 17 ℃, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated group were signi cantly improved (P < 0.05).Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm quality. Besides, the signi cant increase in sow pregnancy rate and piglet birth weight also con rmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrate that the addition of hydroxytyrosol to the diluent can improve the quality of pig sperm and the e ciency of arti cial insemination.

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Supplementation of salvianic acid A to boar semen extender to improve seminal quality and antioxidant capacity

Cited by 11 publications

References 26 publications

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Antioxidant activity and metabolic regulation of sodium salicylate on goat sperm at low temperature

Hydroxytyrosol effectively improves the quality of pig sperm at 17 °C

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