Hydroxytyrosol effectively improves the quality of pig sperm at 17 °C

Abstract

Background: Arti cial insemination technology is the production technology used in more than 99% of the world's pig farms. As the frozen sperm damage is very serious, the preservation of pig sperm is mainly carried out at 17°C. However, the accumulation of a large amount of reactive oxygen species (ROS) during the preservation process is the main reason for the deterioration of sperm quality. In our research, by adding different concentrations of hydroxytyrosol to the diluent during the storage of pig sperm at 17°C, we compared them with the traditional diluent to study the cumulative amount of ROS, the extent of membrane damage, the sperm acrosome integrity, the activity of antioxidant enzymes, and other indicators to evaluate the effect of hydroxytyrosol on the changes of active oxygen species and sperm quality during storage. After we determined the optimal amount of hydroxytyrosol in the diluent, proteomics was used to monitor difference in sperm protein expression between the control group and the addition of 120 μmol/L hydroxytyrosol group (optimum concentration). Ultimately, we selected the control group and the 120 μmol/L hydroxytyrosol group to test the effect of arti cial insemination.Results: The results of our research showed that during storage of pig sperm at 17 ℃, the sperm quality and antioxidant capacity of the hydroxytyrosol-treated group were signi cantly improved (P < 0.05).Proteomics sequencing analysis proved that the addition of hydroxytyrosol treatment group has potential value in improving sperm quality. Besides, the signi cant increase in sow pregnancy rate and piglet birth weight also con rmed that sperm quality during storage can be of vital importance in actual production (P < 0.05).Conclusions: Based on our results, we demonstrate that the addition of hydroxytyrosol to the diluent can improve the quality of pig sperm and the e ciency of arti cial insemination.

Discriminant Analysis and Data Mining CHAID Decision Tree as Tools to Evaluate the Buffering Effect of Hydroxytyrosol on Reactive Oxygen Species in Rooster Sperm Cryopreservation

“…In this same species, Arando et al [48] observed that supplementation of semen with HT improves LPO values of frozen-thawed samples as well as in the case of DHPG and the mixture of both. Similarly, Li et al [51] have reported that the addition of 120 µmol/L of HT during boar semen storage at 17 • C has a positive effect on semen quality.…”

Impact of the Antioxidant Hydroxytyrosol on the Quality of Post-Thawed Stallion Semen

“…Hydroxytyrosol showed antioxidant properties by scavenging ROS, reducing the accumulation of ROS, and promoting the expression of antioxidant enzymes via activating nuclear factor E2-related factor 2 (Nrf2) signaling [ 31 ]. Moreover, hydroxytyrosol enhanced the activity of antioxidant enzymes (CAT, T-AOC, SOD, and GSH-PX), which led to reduced lipid peroxidation (MDA) in pig semen stored at 17°C [ 32 ] and catalyzed the decomposition of hydrogen peroxide (H 2 O 2 ) in postweaning piglets [ 33 ]. Furthermore, Kedechi et al [ 34 ] reported that the addition of hydroxytyrosol reduced sperm DNA oxidation in human semen.…”

Supplementing Boar Diet with Nicotinamide Mononucleotide Improves Sperm Quality Probably through the Activation of the SIRT3 Signaling Pathway

“…However, according to previous studies, improving semen quality positively affects their in vitro and in vivo fertilization ability or reproductive performance. For example, the addition of hydroxytyrosol to a dilution of boar semen improved the artificial insemination efficiency by improving the sperm quality [57]. Moreover, the addition of tretinoin enhanced IVF parameters and spermoocyte binding by improving the boar sperm quality, increasing the cleavage and blastocyst rates [9].…”