Supplementation of cryodiluent media with seaweed or Nile tilapia skin sulfated polysaccharides for freezing of Colossoma macropomum (Characiformes: Serrasalmidae) semen

Pereira, Vanessa Alves; Alencar, Daniel Barroso de; Araújo, Ianna Wivianne Fernandes de; Rodrigues, José Ariévilo Gurgel; Lopes, Júlia Trugilio; Nunes, Larissa Teixeira; Ferreira, Yasmim Maia; Lobato, Jéssica Sales; Montenegro, Assis Rúbens; Salmito‐Vanderley, Carminda Sandra Brito

doi:10.1016/j.aquaculture.2020.735553

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…In the present study, it was observed no relation of membrane integrity with the increase of GAGs concentration, since the mean of sperm with an intact membrane of the control was 78.0 ± 1.06%, while the one of the highest GAGs concentration (5.0 mg mL -1 ) was 69.33 ± 1.69% (P > 0.05; Table 2). Pereira et al (2020) observed good results of sperm membrane integrity when adding GAGs from the skin of O. niloticus to the sperm freezing medium of tambaqui (C. macropomum). In addition, unpublished studies suggest that these GAGs also promote a protective effect on the plasma membrane of P. brevis sperm submitted to vitrification.…”

Section: Donnellymentioning

confidence: 92%

“…The fish configuration with 50 frames per second was used. Analyzes were performed in triplicate and approximately 15" after sperm activation (Pereira et al, 2020). Milt samples with motility above 80% were used to form pools (n = 6), and each pool contained milt from four animals.…”

Section: Analysis Of Fresh Milt and Pool Formationmentioning

confidence: 99%

“…Thus, adding substances with antioxidant action to the freezing medium is important, since the sperm plasma membrane is susceptible to lipid peroxidation during the cryopreservation process. Pereira et al (2020) reported that GAGs from O. niloticus skin protect the plasma membrane of C. macropomum sperm. The same was observed in the present study, as the GAGs did not impair the membrane integrity of P. brevis in any tested concentration.…”

Section: Donnellymentioning

confidence: 99%

“…Studies report that dermatan sulfate can act as an antithrombotic, anticoagulant, and anti-inflammatory agent (Valcarcel et al, 2017); however, there is no investigation on its possible antioxidant action. The application of GAGs in the sperm cryopreservation of fish is very recent, as the study by Pereira et al (2020), who described promising results for the sperm conservation of tambaqui (Colossoma macropomum).…”

Section: Introductionmentioning

confidence: 99%

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Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico

Nascimento

Pereira

Almeida‐Monteiro

et al. 2021

Semina: Ciênc. Agrár.

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The study aimed to evaluate the in vitro antioxidant action of glycosaminoglycans (GAGs) from the skin of Oreochromis niloticus, and to determine their ideal concentration to supplement the sperm freezing medium of Prochilodus brevis. In experiment 1, the in vitro antioxidant properties of GAGs were verified through the analysis of DPPH, chelating ferrous ability, and total antioxidant capacity. In experiment 2, milt pools were formed, which were frozen in solution supplemented or not with different GAGs concentrations: 0 (control), 0.5, 1.0, 1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, or 5.0 mg mL-1 (total of 10 treatments). The samples were evaluated for membrane integrity, DNA integrity, sperm morphology, and sperm kinetics. The results of experiment 1 showed that the GAGs exhibited, with the increase of the concentration, significant antioxidant action, for all the evaluated tests, mainly in the chelating ferrous ability. In experiment 2, it was observed that the increase of GAGs concentration decreased kinetic parameters (P < 0.05), however, the control and 0.5 mg mL-1 GAGs concentration showed similar results. For the other parameters (membrane integrity, DNA integrity, and sperm morphology), there was no decrease in results with the increase of GAGs concentration. In conclusion, GAGs extracted from O. niloticus skin have antioxidant action, and the concentration of 0.5 mg mL-1 was the most adequate to supplement the P. brevis sperm-freezing medium.

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Section: Donnellymentioning

confidence: 92%

Section: Analysis Of Fresh Milt and Pool Formationmentioning

confidence: 99%

Section: Donnellymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico

Nascimento

Pereira

Almeida‐Monteiro

et al. 2021

Semina: Ciênc. Agrár.

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“…Colossoma macropomum (Curvier, 1816), popularly known as tambaqui, is a rheophilic fish of the order Characiformes that is native to the Amazon Basin. In addition to having meat that is appreciated in the market and possessing high commercial value, the species displays adaptability to the captive environment, good feed acceptance, and hardiness (Pereira et al, 2020;Garcez et al, 2021). In 2021, tambaqui led the production of native fish in Brazil, representing 31.2% of national production (Brazilian Association of Pisciculture [PeixeBr], 2022).…”

Section: Introductionmentioning

confidence: 99%

Suplementação do meio criodiluidor com polissacarídeos sulfatados de macroalgas verdes na congelação do sêmen de Colossoma macropomum

et al. 2023

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O estudo teve como objetivo avaliar a adição de polissacarídeos sulfatados (PS) extraídos de duas espécies de macroalgas verdes, Ulva lactuca e Caulerpa racemosa, no meio criodiluidor do sêmen de Colossoma macropomum. Para isso, foram avaliadas quatro concentrações de PS (1,0; 2,0; 3,0 ou 4,0 mg mL-1), de cada macroalga. A coleta de sêmen foi realizada durante o mês de setembro, em Fortaleza, Ceará, Brasil. As amostras de sêmen fresco foram analisadas quanto aos parâmetros de motilidade total dos espermatozoides, velocidade curvilinear (VCL), velocidade em linha reta (VSL), velocidade média do trajeto (VAP), morfologia espermática, integridade de membrana e integridade de DNA. Em seguida, foram criopreservadas em meio de congelação contendo dimetilsulfóxido (DMSO) 10% + glicose 5%, e suplementadas com as diferentes concentrações de PS, tendo ainda um tratamento não suplementado como controle. Após 15 dias, foram descongeladas em banho-maria a 45 ºC por oito segundos, e as mesmas análises do sêmen fresco foram realizadas. Através da análise estatística, os resultados mostraram que não houveram diferenças significativas (p > 0,05) entre as diferentes concentrações de PS testadas para nenhum dos parâmetros avaliados. Já em relação ao controle, não houve diferença nas concentrações (p > 0,05) para a motilidade total, no entanto, para VCL, VSL e VAP, as concentrações de 3,0 e 4,0 mg mL-1 de U. lactuca foram prejudiciais (p < 0,05). O mesmo foi observado em 4,0 mg mL-1 de C. racemosa para VSL e VAP. Para a morfologia, 1,0 e 4,0 mg mL-1 de C. racemosa reduziram os espermatozoides normais (p < 0,05), enquanto para as demais concentrações não houve diferença (p > 0,05). Para todas as concentrações de ambas as macroalgas, a integridade de membrana plasmática foi mantida (p > 0,05). Quanto à integridade do DNA, apenas 4,0 mg mL-1 de U. lactuca foi inferior ao controle (p < 0,05), enquanto as demais concentrações mantiveram o número de espermatozoides com DNA íntegro (p > 0,05). De acordo com os resultados obtidos, concentrações mais elevadas de PS são prejudiciais aos espermatozoides de tambaqui no meio de congelação, enquanto concentrações mais baixas mantiveram parâmetros espermáticos. Estudos posteriores são indicados para melhor avaliar o potencial antioxidante destes polímeros no meio criodiluidor do sêmen de C. macropomum, bem como de outras espécies de peixes.

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Threat and gain: The metabolites of the red algae genus Acanthophora

et al. 2022

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Supplementation of cryodiluent media with seaweed or Nile tilapia skin sulfated polysaccharides for freezing of Colossoma macropomum (Characiformes: Serrasalmidae) semen

Cited by 6 publications

References 40 publications

Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico

Use of glycosaminoglycans from Oreochromis niloticus skin as an antioxidant supplement for milt cryopreservation of Brazilian bocachico

Suplementação do meio criodiluidor com polissacarídeos sulfatados de macroalgas verdes na congelação do sêmen de Colossoma macropomum

Threat and gain: The metabolites of the red algae genus Acanthophora

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