“…Glutaraldehyde has been used for several decades as an effective crosslinking agent for many applications including sample fixation for cytochemistry and electron microscopy (Sabatini et al, 1963), immobilization of enzymes and whole cells (Quiocho and Richards, 1966;Schejter and Bareli, 1970;Walt and Agayn, 1994;Khalaf et al, 1996;Lalonde et al, 1997), and protein crystal stabilization for X-ray crystallography (Wang et al, 1998;Lusty, 1999;Hall et al, 2005;Heras and Martin, 2005;Iimura et al, 2005) as well as for biocatalysis (Margolin, 1996;Zelinski and Waldmann, 1997;Haring and Schreier, 1999;Leisola et al, 2001;Margolin and Navia, 2001;Iimura et al, 2005;Roy and Abraham, 2006). Inspite of its wide applicability and high efficiency as a crosslinking agent, the mechanism and chemistry involved in this process is not fully understood (Whipple and Ruta, 1974;Walt and Agayn, 1994).…”