Superresolution Microscopy with Quantum Emitters

Schwartz, Osip; Levitt, Jonathan M.; Tenne, Ron; Itzhakov, Stella; Deutsch, Zvicka; Oron, Dan

doi:10.1021/nl402552m

Cited by 143 publications

(144 citation statements)

References 37 publications

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“…Yet, intrinsic properties of the fluorophores can equally be utilized for superresolution microscopy. For these intensity correlation microscopy (ICM) techniques, statistically blinking fluorophores [79] or quantum emitters that exhibit antibunching [80] can be used to enhance the resolution. Especially the first approach, known as super-resolution optical fluctuation imaging (SOFI) [79], is widely used in microscopy.…”

Section: And Structured Illuminationmentioning

confidence: 99%

Partial coherence in modern optics: Emil Wolf's legacy in the 21st century

Agarwal

Classen

2020

Progress in Optics

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We highlight the impact of Emil Wolf's work on coherence and polarization on an ever increasing amount of applications in the 21 st century. We present a brief review of how partial coherence at the level of increasing order of coherence functions is leading to evolution in the better methods for microscopy, imaging, optical coherence tomography; speckle imaging; propagation through random media. This evolution in our capabilities is expected to have wide ramifications in Science and Engineering.

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Section: And Structured Illuminationmentioning

confidence: 99%

Partial coherence in modern optics: Emil Wolf's legacy in the 21st century

Agarwal

Classen

2020

Progress in Optics

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“…Sub-Poissonian anti-bunched emission from quantum dots allows superresolved images of a static sample. 68 The missing three-photon coincidence signal enables a spatial resolution that is two thirds of the diffraction limit of a tracking apparatus with an EMCCD camera. These results indicate the possibility of improved temporal bandwidth in comparison to photoactivated localization microscopy and comparable spatial resolution by using even higher order missing coincidences.…”

Section: Tracking Hardwarementioning

confidence: 99%

“…However, this technique requires further suppression of sensor noise to achieve such improvements. 68 The computational expense of localization algorithms for the above measurement 67 and other kinds of non-classical photon statistics 69 might receive further attention with ongoing advances in the signal-to-noise ratios of light sensors.…”

Section: Tracking Hardwarementioning

confidence: 99%

Optical tracking of nanoscale particles in microscale environments

Mathai

Liddle

Stavis

2016

Applied Physics Reviews

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The trajectories of nanoscale particles through microscale environments record useful information about both the particles and the environments. Optical microscopes provide efficient access to this information through measurements of light in the far field from nanoparticles. Such measurements necessarily involve trade-offs in tracking capabilities. This article presents a measurement framework, based on information theory, that facilitates a more systematic understanding of such trade-offs to rationally design tracking systems for diverse applications. This framework includes the degrees of freedom of optical microscopes, which determine the limitations of tracking measurements in theory. In the laboratory, tracking systems are assemblies of sources and sensors, optics and stages, and nanoparticle emitters. The combined characteristics of such systems determine the limitations of tracking measurements in practice. This article reviews this tracking hardware with a focus on the essential functions of nanoparticles as optical emitters and microenvironmental probes. Within these theoretical and practical limitations, experimentalists have implemented a variety of tracking systems with different capabilities. This article reviews a selection of apparatuses and techniques for tracking multiple and single particles by tuning illumination and detection, and by using feedback and confinement to improve the measurements. Prior information is also useful in many tracking systems and measurements, which apply across a broad spectrum of science and technology. In the context of the framework and review of apparatuses and techniques, this article reviews a selection of applications, with particle diffusion serving as a prelude to tracking measurements in biological, fluid, and material systems, fabrication and assembly processes, and engineered devices. In so doing, this review identifies trends and gaps in particle tracking that might influence future research.

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“…An alternative approach, compatible with imaging techniques, uses the diffraction limit of the imaging system to split the optical signal between several detectors in an array, and correlates their output [16,27,46,51] ( Figure 1(b)). While commercial low light cameras such as intensified cameras and electron multiplying charge coupled devices (EMCCD) are natural candidates to perform such tasks, they operate only at relatively low ∼ kHz frame rates.…”

Section: Introductionmentioning

confidence: 99%

“…your signal level is at most a single reading (e.g. a simultaneous photon pair) per frame per diffraction limited spot [46,52,56]. As a result, an imaging detector with a ∼ MHz readout rate is extremely beneficial to acquire the quantum contrast within reasonable exposure times.…”

Section: Introductionmentioning

confidence: 99%

Quantum correlation measurement with single photon avalanche diode arrays

Lubin¹,

Tenne²,

Antolović³

et al. 2019

Opt. Express

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Temporal photon correlation measurement, instrumental to probing the quantum properties of light, typically requires multiple single photon detectors. Progress in single photon avalanche diode (SPAD) array technology highlights their potential as high performance detector arrays for quantum imaging and photon number resolving (PNR) experiments. Here, we demonstrate this potential by incorporating a novel on-chip SPAD array with 55% peak photon detection probability, low dark count rate and crosstalk probability of 0.14% per detection, in a confocal microscope. This enables reliable measurements of second and third order photon correlations from a single quantum dot emitter. Our analysis overcomes the inter-detector optical crosstalk background even though it is over an order of magnitude larger than our faint signal. To showcase the vast application space of such an approach, we implement a recently introduced super-resolution imaging method, quantum image scanning microscopy (Q-ISM).

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Superresolution Microscopy with Quantum Emitters

Cited by 143 publications

References 37 publications

Partial coherence in modern optics: Emil Wolf's legacy in the 21st century

Partial coherence in modern optics: Emil Wolf's legacy in the 21st century

Optical tracking of nanoscale particles in microscale environments

Quantum correlation measurement with single photon avalanche diode arrays

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