Superparamagnetic nano-composite scaffolds for promoting bone cell proliferation and defect reparation without a magnetic field

Effect of poling state and morphology of piezoelectric poly(vinylidene fluoride) membranes for skeletal muscle tissue engineering

“…Thereafter, the samples were again washed with PBS and dehydrated using an ethanol gradient (30, 50, 60, 70, 80, 90 and 100 % ethanol in water). Afterwards, the samples were placed in 40 vacuum at room temperature for 4 h. The dried samples were then gold sputtered in vacuum and examined by SEM.…”

“…Surface charge has been recently described as an important 40 parameter on the cell attachment and proliferation 47 . Previous investigations have demonstrated the influence of the polarization of electroactive PVDF on biological cell response.…”

“…Tissue engineered skeletal 35 muscles have large potential in applications related to muscular dysfunctions, such as muscular dystrophy 14 and cardiac infarct 15 . In this field, the principles of cell biology, material science and biomedical engineering are being applied towards the creation of biological substitutes that will restore and maintain standard 40 function in diseased and injured tissues/organs 16 . Tissue engineering requires three main elements to create a functional substitute for organs: cells, culture modes and scaffolds 12 .…”

“…The main issues to be clarified are the effect of 35 the surface charge on cell adhesion and proliferation and to verify the effect of sample morphology on the attachment, spreading and myoblast morphology. In order to study the effect of surface charge, three kinds of -PVDF films were used: non-poled -cell culture on non charged surface; "poled +" -cell culture on the 40 positively charged side of the sample; and "poled -" -cell culture on the negatively charged side of the sample -; and for the study the effect of fiber orientation on the myoblast morphology, two types of electrospun PVDF fibers, randomly oriented and aligned, were used. 45 …”

Magnetoactive electrospun nanofibers in tissue engineering applications

“…These results demonstrate the high potential of magnetoactive polymer-based fibrous bundles as a scaffold promoting cell growth and as a cargo for the magnetic field-induced generation of highly oriented 3-D cell-dense tissues. The performance of magnetoresponsive fibrous mats on bone regeneration was also studied by K. Lai et al (2012) who have reported on the fabrication of magnetic biocompatible fibrous scaffolds consisting of poly(lactic-co-glycolic acid) (PLGA) and superparamagnetic Fe3O4 NPs with variable nanoparticle loading. The authors studied their effect on different bone cells (Rpse17/1.8 and MC3T3-E1) in the absence of an external magnetic field.…”