Superparamagnetic iron oxide nanoparticles functionalized by peptide nucleic acids

Galli, Marco; Guerrini, Andrea; Cauteruccio, Silvia; Thakare, P.; Dova, D.; Orsini, F.; Arosio, Paolo; Carrara, C.; Sangregorio, Claudio; Lascialfari, A.; Maggioni, Daniela; Licandro, Emanuela

doi:10.1039/c7ra00519a

Cited by 47 publications

(35 citation statements)

References 66 publications

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“…The entrance of nanomaterials into cell membranes is inevitable for applications in drug delivery, magnetic hyperthermia, transfection and MRI imaging of cells. [1][2][3][4] The interaction between nanoparticles and cell membranes is impacted by the surface modication, charge, size and shape of the nanoparticles. [5][6][7] Phospholipids are important components of mammalian cell membranes.…”

Section: Introductionmentioning

confidence: 99%

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Zhang

Huang

et al. 2017

RSC Adv.

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Section: Introductionmentioning

confidence: 99%

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Zhang

Huang

et al. 2017

RSC Adv.

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“…Indeed, the two sharp and strong bands at 1488 cm -1 and 950 cm -1 , characteristic of TMAOH (CH3 asymmetric deformation mode and of the C-N asymmetric stretching [57], Figure S6 middle) were no more visible in the SPION@ISA23-ND spectrum ( Figure S6 bottom). The latter spectrum showed both the typical bands of polyamidoamine and the intense vibrational band due to iron oxide core, centred at 573 cm -1 (Fe-O-Fe stretching modes of the spinel structure of magnetite) [58].…”

Section: Two-step Ligand Exchangementioning

confidence: 99%

“…A PAA bearing a thiol group in 10% of its repeating units (ISA23SH10% in Scheme S4 top) was first reacted with nitrodopamine, following the same procedure described above for ISA23, resulting in functionalization of ~10 % of the free carboxyl groups (Scheme S4). In a second step, Michael addition of the ISA23SH thiol groups with the ethylmaleimido group of the functionalized-RhodB[58] produced ISA23SH-ND-RhodB polymer (Scheme S4 bottom), whose UV-vis spectrum is reported inFigure S8b. At 530 and 562 nm the typical peaks due to RhodB fragment were observed, while the bands due to the nitrodopamine pendent lie between 340 and 460 nm.…”

mentioning

confidence: 99%

A new catechol-functionalized polyamidoamine as an effective SPION stabilizer

Galli

Rossotti

Arosio

et al. 2019

Colloids and Surfaces B: Biointerfaces

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Highlights    Catechol-functionalization of a polyamidoamine for tight linkage to SPION surface size 100±28 nm). SPION@ISA23-ND were re-suspended after lyophilization reverting to their pristine dimensions. The SPION@ISA23-ND adsorption of BSA in water, considered as the first stage of phagocytosis, was very low, suggesting that ISA23 could impart stealthiness to SPION@ISA23-ND. 1 H-NMR relaxivity measurements showed an r2 value of 158 s-1 mmol-1 L (vs 100 s-1 mmol-1 L for Endorem) at relevant clinical fields for magnetic resonance imaging (from 0.2 to 1.5 T). SPION@ISA23-ND was tested on HeLa cells and their internalization was visualized by reflectance microscopy. Finally, with the aim of prepare a new dual magneto-optical system, a synthetic procedure to decorate SPION@ISA23-ND with a fluorescent dye was devised, even though the emission intensity of the resultant conjugate was lower than expected, possibly due to luminescence quenching caused by the closeness of emitting moieties to the SPION surface.

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“…MIONPs make it possible to efficiently deliver drugs to the brain tumour cells. MIONPs in the gene delivery system loads the therapeutic genes for escorting them to the diseased sites to facilitate their shuttling across the target cell membranes and to ultimately release them intracellularly to accomplish their functions [142,145,146].…”

Section: Cancer Treatmentmentioning

confidence: 99%

Multifunctional magnetic iron oxide nanoparticles: diverse synthetic approaches, surface modifications, cytotoxicity towards biomedical and industrial applications

Natarajan

Harini

Gajula³

et al. 2019

BMC Mat

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Magnetic iron oxide nanoparticles (MIONPs) play a major role in the emerging fields of nanotechnology to facilitate rapid advancements in biomedical and industrial platforms. The superparamagnetic properties of MIONPs and their environment friendly synthetic methods with well-defined particle size have become indispensable to obtain their full potential in a variety of applications ranging from cellular to diverse areas of biomedical science. Thus, the broadened scope and need for MIONPs in their demanding fields of applications required to be highlighted for a comprehensive understanding of their state-of-the-art. Many synthetic methods, however, do not entirely abolish their undesired cytotoxic effects caused by free radical production and high iron dosage. In addition, the agglomeration of MIONPs has also been a major problem. To alleviate these issues, suitable surface modification strategies adaptive to MIONPs has been suggested not only for the effective cytotoxicity control but also to minimize their agglomeration. The surface modification using inorganic and organic polymeric materials would represent an efficient strategy to utilize the diagnostic and therapeutic potentials of MIONPs in various human diseases including cancer. This review article elaborates the structural and magnetic properties of MIONPs, specifically magnetite, maghemite and hematite, followed by the important synthetic methods that can be exploited for biomedical approaches. The in vivo cytotoxic effects and the possible surface modifications employed to eliminate the cytotoxicity thereby enhancing the nanoparticle efficacy are also critically discussed. The roles and applications of surface modified MIONPs in medical and industrial platforms have been described for the benefits of global well-being.

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Superparamagnetic iron oxide nanoparticles functionalized by peptide nucleic acids

Abstract: Hydrophilic SPION were decorated with PNA decamers by SH/maleimide clickreaction as potential MRI and hyperthermia agents, and PNA carriers.

Cited by 47 publications

References 66 publications

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

A new catechol-functionalized polyamidoamine as an effective SPION stabilizer

Multifunctional magnetic iron oxide nanoparticles: diverse synthetic approaches, surface modifications, cytotoxicity towards biomedical and industrial applications

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