Superparamagnetic Fe3O4-PEG2K-FA@Ce6 Nanoprobes for in Vivo Dual-mode Imaging and Targeted Photodynamic Therapy

Yin, Ting; Huang, Peng; Gao, Guo; Shapter, Joseph G.; Shen, Yulan; Sun, Rongjin; Yue, Caixia; Zhang, Chunlei; Liu, Yanlei; Zhou, Shihua; Cui, Daxiang

doi:10.1038/srep36187

Cited by 35 publications

(22 citation statements)

References 34 publications

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“…1 a-b) , close to the calculated diameter of 7.57 nm from the strongest peak (311) observed in XRD (Figure 1 c). Figure 1 c also shows that the XRD pattern of the synthesized nanoparticles is in good agreement with that of the standard cubic phase structure 26 . And then, the value of T2 relaxation rate Fe 3 O 4 NPs was 264.6 mM -1 s -1 (Fig.…”

Section: Resultssupporting

confidence: 71%

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Zhou¹,

Yang²,

Zou³

et al. 2017

Theranostics

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Urethral strictures remain a reconstructive challenge, due to less than satisfactory outcomes and high incidence of stricture recurrence. An “ideal” urethral reconstruction should establish similar architecture and function as the original urethral wall. We fabricated a novel tissue-engineered bionic urethras using cell sheet technology and report their viability in a canine model. Small amounts of oral and adipose tissues were harvested, and adipose-derived stem cells, oral mucosal epithelial cells, and oral mucosal fibroblasts were isolated and used to prepare cell sheets. The cell sheets were hierarchically tubularized to form 3-layer tissue-engineered urethras and labeled by ultrasmall super-paramagnetic iron oxide (USPIO). The constructed tissue-engineered urethras were transplanted subcutaneously for 3 weeks to promote the revascularization and biomechanical strength of the implant. Then, 2 cm length of the tubularized penile urethra was replaced by tissue-engineered bionic urethra. At 3 months of urethral replacement, USPIO-labeled tissue-engineered bionic urethra can be effectively detected by MRI at the transplant site. Histologically, the retrieved bionic urethras still displayed 3 layers, including an epithelial layer, a fibrous layer, and a myoblast layer. Three weeks after subcutaneous transplantation, immunofluorescence analysis showed the density of blood vessels in bionic urethra was significantly increased following the initial establishment of the constructs and was further up-regulated at 3 months after urethral replacement and was close to normal level in urethral tissue. Our study is the first to experimentally demonstrate 3-layer tissue-engineered urethras can be established using cell sheet technology and can promote the regeneration of structural and functional urethras similar to normal urethra.

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Section: Resultssupporting

confidence: 71%

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Zhou¹,

Yang²,

Zou³

et al. 2017

Theranostics

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“…This evaluation was considered important as MR imaging is a powerful imaging tool that is widely used for non-invasive diagnoses owing to its spatial resolution and depth of tissue penetration [7]. Iron oxide nanoparticles have long been used as T 2 contrast agents [48,49]. To analyze in vitro MR imaging via FCF NPs, MRI contrasts of four cancer cell types were detected following incubation with concentrations of 0 to 220 µg/mL of FCF NPs for 2 h, using a 3.0 tesla MRI instrument.…”

Section: In Vitro Mr Imaging Of Fcf Nps In Various Cancer Cellsmentioning

confidence: 99%

Photo-Functionalized Magnetic Nanoparticles as a Nanocarrier of Photodynamic Anticancer Agent for Biomedical Theragnostics

Nam

Han

Lee³

et al. 2020

Cancers

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Various theragnostic agents have been devised and developed as cancer treatments; however, existing agents are often limited by their specific functions and complexities. Here, we report multifunctional magnetite (Fe3O4) nanoparticles functionalized with chlorin e6 (Ce6) and folic acid (FA) using a simple fabrication process to be used as theragnostic agents in photodynamic therapy (PDT). The effectiveness of cellular uptake of Fe3O4-Ce6-FA nanoparticles (FCF NPs) and its visualization as well as the photodynamic anticancer activities were evaluated. The mechanism of cancer cell death by the FCF NPs was also verified with qualitative and quantitative methods. Results indicate that FCF NPs have good penetration efficacy, resulting in excellent in vitro fluorescence and magnetic resonance imaging in cancer cells. FCF NPs exhibited promising anticancer activity in an irradiation time- and FCF NPs-dose-dependent manner in various cancer cell lines, leading to apoptotic cell death via morphological changes in cell membrane, nuclear, and DNA damage, and via overexpression of apoptosis-related genes, such as ZFP36L1, CYR61, GADD45G, caspases-2, -3, -9, 10, and -14. This study suggests that FCF NPs may be safely used in cancer therapy via PDT and could be a versatile therapeutic tool and biocompatible theragnostic agent, which may be used in diagnostic imaging.

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“…Attaching these small molecules to SPIO increases their blood circulation half-life, promotes their tumor retention through the EPR effect and enables real-time in vivo drug tracking with MRI [20]. Although SPIO nanoparticles are normally metabolized in the liver, they were also found to accumulate in other organs of the reticuloendothelial system (e.g., spleen) [21,22], where the drug conjugates can exert potential toxic effects especially after multiple dose administration. To minimize off-target toxicity of SPIO-conjugated cytotoxic drugs to normal organs, several design principles have been employed, including pH-, temperature- and enzyme-mediated drug release in tumors.…”

Section: Spio-delivered Chemotherapymentioning

confidence: 99%

Next-generation superparamagnetic iron oxide nanoparticles for cancer theranostics

Nejadnik

Daldrup‐Link

2017

Drug Discovery Today

117

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Superparamagnetic nanoparticles of iron oxides (SPIO) have been intensively studied for the development of contrast agents in magnetic resonance imaging (MRI). First generation SPIO had diagnostic capabilities only, while a new generation of SPIO have multifunctional characteristics for combined therapeutic and diagnostic applications. These theranostic nanoparticles hold great potential for image-guided cancer therapies. Especially, polymer-coated theranostic SPIO have enjoyed increasing attention due to good biocompatibility, biodegradability and versatile functionality endowed by polymeric matrices. This review provides an overview of recently developed polymer-coated multifunctional SPIO for cancer theranostics and discusses current challenges and future perspectives.

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Superparamagnetic Fe3O4-PEG2K-FA@Ce6 Nanoprobes for in Vivo Dual-mode Imaging and Targeted Photodynamic Therapy

Cited by 35 publications

References 34 publications

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Fabrication of Tissue-Engineered Bionic Urethra Using Cell Sheet Technology and Labeling By Ultrasmall Superparamagnetic Iron Oxide for Full-Thickness Urethral Reconstruction

Photo-Functionalized Magnetic Nanoparticles as a Nanocarrier of Photodynamic Anticancer Agent for Biomedical Theragnostics

Next-generation superparamagnetic iron oxide nanoparticles for cancer theranostics

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