Superoxide production in aprotic interior of chloroplast thylakoids

Takahashi, Masaaki; Asada, Kozi

doi:10.1016/0003-9861(88)90080-x

Cited by 141 publications

(96 citation statements)

References 32 publications

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“…6). First, the production of O 2 2 and OH$ at a secondary electron acceptor in PSI induces PSI photoinhibition (Takahashi and Asada 1988;Sonoike et al, 1995). PSI has four electron acceptors (A 0 as a primary acceptor, and A 1 , F X , and F A /F B as secondary acceptors).…”

Section: Discussionmentioning

confidence: 99%

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Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition

et al. 2016

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Photosystem I (PSI) photoinhibition suppresses plant photosynthesis and growth. However, the mechanism underlying PSI photoinhibition has not been fully clarified. In this study, in order to investigate the mechanism of PSI photoinhibition in higher plants, we applied repetitive short-pulse (rSP) illumination, which causes PSI-specific photoinhibition in chloroplasts isolated from spinach leaves. We found that rSP treatment caused PSI photoinhibition, but not PSII photoinhibition in isolated chloroplasts in the presence of O 2 . However, chloroplastic superoxide dismutase and ascorbate peroxidase activities failed to protect PSI from its photoinhibition. Importantly, PSI photoinhibition was largely alleviated in the presence of methyl viologen, which stimulates the production of reactive oxygen species (ROS) at the stromal region by accepting electrons from PSI, even under the conditions where CuZn-superoxide dismutase and ascorbate peroxidase activities were inactivated by KCN. These results suggest that the ROS production site, but not the ROS production rate, is critical for PSI photoinhibition. Furthermore, we found that not only superoxide (O 2 2 ) but also singlet oxygen ( 1 O 2 ) is involved in PSI photoinhibition induced by rSP treatment. From these results, we suggest that PSI photoinhibition is caused by both O 2 2 and 1 O 2 produced within the thylakoid membranes when electron carriers in PSI become highly reduced. Here, we show, to our knowledge, new insight into the PSI photoinhibition in higher plants.

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Section: Discussionmentioning

confidence: 99%

“…S4). In fact, Takahashi and Asada (1988) reported that O 2 reduction detected by O 2 2 -dependent cytochrome c reduction was stimulated in thylakoid membranes that were disintegrated by detergent. This means that the O 2 reduction site does not exist on the thylakoid membrane surface.…”

Section: Discussionmentioning

confidence: 99%

Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition

et al. 2016

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“…see [6]). The data of the two early studies [7,18] revealed a delay in the appearance of O 2 ÅÀ outside thylakoids after its generation in the light. These data are in line with the above considerations.…”

Section: Discussionmentioning

confidence: 99%

Production of superoxide in chloroplast thylakoid membranes

et al. 2011

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a b s t r a c tAccumulation of nitroxide radicals, DCP Å or TMT Å , under illumination of a thylakoid suspension containing either hydrophilic, DCP-H, or lipophilic, TMT-H, cyclic hydroxylamines that have high rate constants of the reaction with superoxide radicals, was measured using ESR. A slower accumulation of TMT Å in contrast with DCP Å accumulation was explained by re-reduction of TMT Å by the carriers of the photosynthetic electron transport chain within the membrane. Superoxide dismutase suppressed TMT Å accumulation to a lesser extent than DCP Å accumulation. The data are interpreted as evidencing the production of intramembrane superoxide in thylakoids.

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“…7). Takahashi et al (1988) showed that in PSII, in the places of plastoquinone Q A and Q B activity, electron leak may occur, accompanied by a reduction of molecular oxygen to superoxide radical, further dismutated to H 2 O 2 . Another possible source of ROS generation during R1 could be PSI, where overloading of the electron transport chain (ETC) could cause a redirection of some electrons from ferredoxin to O 2 and reducing it via Mehler reaction to superoxide radical, which was then spontaneously dismutated to H 2 O 2 by Cu/Zn-SOD (Polle 1996;Edreva 2005).…”

Section: (D)mentioning

confidence: 99%

Rapid plant rehydration initiates permanent and adverse changes in the photosynthetic apparatus of triticale

Hura

Ostrowska

et al. 2015

Plant Soil

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Background and aims The reasons for partial recovery after a soil drought are not fully understood and have not been studied so far. This study investigated the physiological and biochemical responses of triticale cultivars with differential recovery ability after soil water deficit. Methods Activity of the photosynthetic apparatus under soil drought followed by rehydration was estimated. Plant antioxidant potential was determined based on the measurement of catalase and peroxidase activity. The levels of hydrogen peroxide and superoxide radical were assessed. Results Under rehydration, the not fully-recovered cultivar experienced further significant increase in the content of H 2 O 2 and inhibited activity of the photosynthetic apparatus, as compared to the drought period. On 42 nd day of the rehydration, the not fully-recovered cultivar showed also a reduced photosynthetic activity in the flag leaves, which resulted in a significant decrease in its grain yield. The first week of a rapid rehydration involved a decrease in total peroxidase and catalase activities. The increased content of H 2 O 2 was compensated only when leaf water content was gradually restored in the first week of the rehydration and no further decrease in the activity of the photosynthetic apparatus was noticed. Conclusions A destructive effect of the rapid rehydration was manifested in an intensification of the physiological processes associated with reactive oxygen species (ROS) overproduction. An important cause of hydrogen peroxide overproduction seems to be the electron leakage due to overloading of the electron transport chain (ETC) in the PSI and PSII.

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Superoxide production in aprotic interior of chloroplast thylakoids

Cited by 141 publications

References 32 publications

Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition

Superoxide and Singlet Oxygen Produced within the Thylakoid Membranes Both Cause Photosystem I Photoinhibition

Production of superoxide in chloroplast thylakoid membranes

Rapid plant rehydration initiates permanent and adverse changes in the photosynthetic apparatus of triticale

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