Production of superoxide in chloroplast thylakoid membranes

Kozuleva, Marina; Klenina, I. B.; Proskuryakov, I. I.; Kirilyuk, Igor A.; Иванов, Б. Н.

doi:10.1016/j.febslet.2011.03.004

Cited by 41 publications

(13 citation statements)

References 20 publications

(29 reference statements)

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“…The main site of O 2 •− generation is PSI. Several experiments revealed that PhQ could be the major contributor to this process (Kruk et al, 2003;Kozuleva et al, 2011Kozuleva et al, , 2014 •− appearance within the thylakoid membrane (Kozuleva et al, 2011); however, a significant part of O 2 •− formed by PhQ •− still likely diffuses outside the membrane. Nevertheless, the increasing irradiance resulted in both a larger O 2…”

Section: Discussionmentioning

confidence: 99%

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Minimizing an Electron Flow to Molecular Oxygen in Photosynthetic Electron Transfer Chain: An Evolutionary View

et al. 2020

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Recruitment of H 2 O as the final donor of electrons for light-governed reactions in photosynthesis has been an utmost breakthrough, bursting the evolution of life and leading to the accumulation of O 2 molecules in the atmosphere. O 2 molecule has a great potential to accept electrons from the components of the photosynthetic electron transfer chain (PETC) (so-called the Mehler reaction). Here we overview the Mehler reaction mechanisms, specifying the changes in the structure of the PETC of oxygenic phototrophs that probably had occurred as the result of evolutionary pressure to minimize the electron flow to O 2 . These changes are warranted by the fact that the efficient electron flow to O 2 would decrease the quantum yield of photosynthesis. Moreover, the reduction of O 2 leads to the formation of reactive oxygen species (ROS), namely, the superoxide anion radical and hydrogen peroxide, which cause oxidative stress to plant cells if they are accumulated at a significant amount. From another side, hydrogen peroxide acts as a signaling molecule. We particularly zoom in into the role of photosystem I (PSI) and the plastoquinone (PQ) pool in the Mehler reaction.

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Section: Discussionmentioning

confidence: 99%

“…•− , which can be detected outside the membrane when diffused there or can be detected within the thylakoid membranes (Kozuleva et al, 2011). The value of E m (O 2/ O 2 •− ) in water is −160 mV, while in hydrophobic zones of proteins and membranes it is more negative, approximately −550 mV (Wardman, 1990).…”

Section: Introductionmentioning

confidence: 99%

Minimizing an Electron Flow to Molecular Oxygen in Photosynthetic Electron Transfer Chain: An Evolutionary View

et al. 2020

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“…It was suggested that O2 •− production within the thylakoid membranes most likely occurs via autooxidation of the membrane-bound primary electron acceptors in PSI, possibly 4Fe-4S clusters (FX, FA, and FB) [152]. The Em of FA/FA − and FB/FB − and [145,[268][269][270][271]; (C) possible means of ROS formation in PSI [152,266,[272][273][274][275]. PC is plastocyanin; P700 is a dimer of Chl a molecules, the primary electron donor; A 0A and A 0B are Chl a molecules located in branches A and B, respectively, both act as primary electron acceptors; A 1A and A 1B are phylloquinone molecules located in branch A and B, respectively, both acting as electron acceptors; F X , a 4Fe-4S cluster, a secondary electron acceptor; F A and F B , 4Fe-4S clusters, terminal electron acceptors.…”

Section: Formation Of Reduced Forms Of Oxygenmentioning

confidence: 99%

Oxygen and ROS in Photosynthesis

Khorobrykh

Havurinne

Mattila

et al. 2020

Plants

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Oxygen is a natural acceptor of electrons in the respiratory pathway of aerobic organisms and in many other biochemical reactions. Aerobic metabolism is always associated with the formation of reactive oxygen species (ROS). ROS may damage biomolecules but are also involved in regulatory functions of photosynthetic organisms. This review presents the main properties of ROS, the formation of ROS in the photosynthetic electron transport chain and in the stroma of chloroplasts, and ROS scavenging systems of thylakoid membrane and stroma. Effects of ROS on the photosynthetic apparatus and their roles in redox signaling are discussed.

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“…There, superoxide is rapidly dismutated to the more stable hydrogen peroxide by superoxide dismutase (SOD) [28]. If superoxide is produced within the thylakoid membrane [48] where SOD is absent, hydrogen peroxide can be produced by the reduction of superoxide by plastohydroquinone PQH 2 [47,49], and the same pathway also occurs within mitochondrial membrane [50]. …”

Section: Introductionmentioning

confidence: 99%

Production of superoxide from Photosystem II in a rice (Oryza sativaL.) mutant lacking PsbS

Zulfugarov

Tovuu

et al. 2014

BMC Plant Biol

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BackgroundPsbS is a 22-kDa Photosystem (PS) II protein involved in non-photochemical quenching (NPQ) of chlorophyll fluorescence. Rice (Oryza sativa L.) has two PsbS genes, PsbS1 and PsbS2. However, only inactivation of PsbS1, through a knockout (PsbS1-KO) or in RNAi transgenic plants, results in plants deficient in qE, the energy-dependent component of NPQ.ResultsIn studies presented here, under fluctuating high light, growth of young seedlings lacking PsbS is retarded, and PSII in detached leaves of the mutants is more sensitive to photoinhibitory illumination compared with the wild type. Using both histochemical and fluorescent probes, we determined the levels of reactive oxygen species, including singlet oxygen, superoxide, and hydrogen peroxide, in leaves and thylakoids. The PsbS-deficient plants generated more superoxide and hydrogen peroxide in their chloroplasts. PSII complexes isolated from them produced more superoxide compared with the wild type, and PSII-driven superoxide production was higher in the mutants. However, we could not observe such differences either in isolated PSI complexes or through PSI-driven electron transport. Time-course experiments using isolated thylakoids showed that superoxide production was the initial event, and that production of hydrogen peroxide proceeded from that.ConclusionThese results indicate that at least some of the photoprotection provided by PsbS and qE is mediated by preventing production of superoxide released from PSII under conditions of excess excitation energy.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0242-2) contains supplementary material, which is available to authorized users.

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Production of superoxide in chloroplast thylakoid membranes

Cited by 41 publications

References 20 publications

Minimizing an Electron Flow to Molecular Oxygen in Photosynthetic Electron Transfer Chain: An Evolutionary View

Minimizing an Electron Flow to Molecular Oxygen in Photosynthetic Electron Transfer Chain: An Evolutionary View

Oxygen and ROS in Photosynthesis

Production of superoxide from Photosystem II in a rice (Oryza sativaL.) mutant lacking PsbS

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