Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples

Xu, Chunli; Liu, Shu; Liu, Zhiqiang; Song, Fengrui; Liu, Shuying

doi:10.1016/j.aca.2013.07.027

Cited by 45 publications

(33 citation statements)

References 32 publications

(39 reference statements)

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“…SOD activity was determined by the ability of colonic SOD to inhibit the auto-oxidation of pyrogallol, as previously described [49]. The change in absorbance at 420 nm was measured at 1 min intervals for 3 min.…”

Section: Methodsmentioning

confidence: 99%

Swimming attenuates inflammation, oxidative stress, and apoptosis in a rat model of dextran sulfate sodium-induced chronic colitis

et al. 2016

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Increasing evidence suggests that regular physical exercise suppresses chronic inflammation. However, the potential inhibitory effects of swimming on dextran sulfate sodium (DSS)-induced chronic colitis, and its underlying mechanisms, remain unclear. In this study, rats were orally administered DSS to induce chronic colitis, and subsequently treated with or without swimming exercise. A 7-week swimming program (1 or 1.5 hours per day, 5 days per week) ameliorated DSS-caused colon shortening, colon barrier disruption, spleen enlargement, serum LDH release, and reduction of body weight gain. Swimming for 1.5 hours per day afforded greater protection than 1 hour per day. Swimming ameliorated DSS-induced decrease in crypt depth, and increases in myeloperoxidase activity, infiltration of Ly6G+ neutrophils and TNF-a- and IFN-?-expressing CD3+ T cells, as well as fecal calprotectin and lactoferrin. Swimming inhibited pro-inflammatory cytokine and chemokine production and decreased the protein expression of phosphorylated nuclear factor-?B p65 and cyclooxygenase 2, whereas it elevated interleukin-10 levels. Swimming impeded the generation of reactive oxygen species, malondialdehyde, and nitric oxide; however, it boosted glutathione levels, total antioxidant capacity, and superoxide dismutase and glutathione peroxidase activities. Additionally, swimming decreased caspase-3 activity and expression of apoptosis-inducing factor, cytochrome c, Bax, and cleaved-caspase-3, but increased Bcl-2 levels. Overall, these results suggest that swimming exerts beneficial effects on DSS-induced chronic colitis by modulating inflammation, oxidative stress, and apoptosis.

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Section: Methodsmentioning

confidence: 99%

Swimming attenuates inflammation, oxidative stress, and apoptosis in a rat model of dextran sulfate sodium-induced chronic colitis

et al. 2016

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“…The superoxide radical scavenging activity was determined by the Autoxidation of Pyrogallol method (Xu et al, 2013). The hydroxyl radical scavenging activity (HRSA) and hydrogen peroxide scavenging activity (HPSA) of the samples was measured according to the method of Xie et al (2011).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Effect of different ripening stages on bioactive compounds and antioxidant capacity of wild Rosa laevigata Michx

Xie

Wang

et al. 2016

Food Sci. Technol

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Rosa Laevigata Michx., contents of bioactive components contribute to the health beneftis of consuming fresh, snacks and medicine, is widely used as the Chinese medicinal herbs. The effect of fruit ripening on the bioactive compounds and antioxidant capacity of Rosa laevigata Michx fruits was investigated. The results showed that, the bioactive compounds, including titratable acidity, reducing sugar, total ascorbic acid, total polyphenol and total flavonoids, significant changes occurred at different ripening stages. The contents of reducing sugar, total polyphenol and total flavonoids were directly proportional to the maturity. However, the content of titration acid and ascorbic acid is inversely proportional to the maturity. Antioxidant capacity and RDS inhibition are proportional to the maturity, and the antioxidant capacity of Rosa laevigata Michx significantly increase during maturity. The Rosa laevigata Michx fruits at fully ripe stage had higher healthcare and medicinal value.Keywords: Rosa laevigata Michx.; ripening stages; bioactive compounds; antioxidant capacity.Practical Application: Dptimizing harvesting and postharvest management of Rosa laevigata Michx.

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“…; it was demonstrated that WST-1 generated an approximately twofold greater increase in absorbance than ferricytochrome C at their respective wavelengths [96]. (Figure 5B), and luminol is oxidized using a peroxidase such as myeloperoxidase (MPO) or horseradish peroxidase (HRP) ( Figure 5C).…”

Section: •−mentioning

confidence: 99%

Involvement of Free Radicals in the Development and Progression of Alzheimer’s Disease

Rosales‐Hernández¹,

Rodríguez²,

Correa-Basurto³

2016

Free Radicals and Diseases

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Alzheimer's disease (AD) is a major dementia related to an overproduction of free radicals (FRs), which leads to the generation of oxidative stress in brain tissue. Amyloid beta-peptide of 42 amino acid residues (Aβ 1-42) is the main source of FRs in patients with AD. βA 1-42 results from hydrolysis of the amyloid precursor protein by β-secretase in a process known as the amyloidogenic pathway. During βA 1-42 aggregation, the peptide interacts with various transition metals to produce hydrogen peroxide (H 2 O 2) by the Fenton reaction, generating the hydroxyl radical (• OH), which damages lipids, proteins, and nucleic acids, thereby contributing to neurodegeneration. In addition, βA 1-42 is recognized by microglial receptors; it activates these cells, causing overproduction of superoxide anion (O 2 •−) by NADPH oxidase; O 2 •− is also converted into H 2 O 2 and finally to • OH in the Fenton reaction. Other factors that contribute to oxidative stress during microglial activation are the overproduction of nitric oxide and interleukins and the overexpression of some enzymes, including cyclooxygenase and inducible nitric oxide synthase, all of which contribute to FR production. Currently, various models in vitro and in vivo exist that permit quantification of O 2 •− and H 2 O 2 and determination of the effects of these reactive oxygen species.

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Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: A simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples

Cited by 45 publications

References 32 publications

Swimming attenuates inflammation, oxidative stress, and apoptosis in a rat model of dextran sulfate sodium-induced chronic colitis

Swimming attenuates inflammation, oxidative stress, and apoptosis in a rat model of dextran sulfate sodium-induced chronic colitis

Effect of different ripening stages on bioactive compounds and antioxidant capacity of wild Rosa laevigata Michx

Involvement of Free Radicals in the Development and Progression of Alzheimer’s Disease

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